scholarly journals Hypovirus-Responsive Transcription Factor Gene pro1 of the Chestnut Blight Fungus Cryphonectria parasitica Is Required for Female Fertility, Asexual Spore Development, and Stable Maintenance of Hypovirus Infection

2008 ◽  
Vol 8 (3) ◽  
pp. 262-270 ◽  
Author(s):  
Qihong Sun ◽  
Gil H. Choi ◽  
Donald L. Nuss
Keyword(s):  
Transcription Factor ◽  
Cryphonectria Parasitica ◽  
Female Fertility ◽  
Chestnut Blight ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Disruption Mutant ◽  
Chestnut Blight Fungus ◽  
Stable Maintenance

ABSTRACT We report characterization of the gene encoding putative transcription factor PRO1, identified in transcriptional profiling studies as being downregulated in the chestnut blight fungus Cryphonectria parasitica in response to infection by virulence-attenuating hypoviruses. Sequence analysis confirmed that pro1 encodes a Zn(II)2Cys6 binuclear cluster DNA binding protein with significant sequence similarity to the pro1 gene product that controls fruiting body development in Sordaria macrospora. Targeted disruption of the C. parasitica pro1 gene resulted in two phenotypic changes that also accompany hypovirus infection, a significant reduction in asexual sporulation that could be reversed by exposure to high light intensity, and loss of female fertility. The pro1 disruption mutant, however, retained full virulence. Although hypovirus CHV1-EP713 infection was established in the pro1 disruption mutant, infected colonies continually produced virus-free sectors, suggesting that PRO1 is required for stable maintenance of hypovirus infection. These results complement the recent characterization of the hypovirus-responsive homologue of the Saccharomyces cerevisiae Ste12 C2H2 zinc finger transcription factor gene, cpst12, which was shown to be required for C. parasitica female fertility and virulence.

scholarly journals Ste12 Transcription Factor Homologue CpST12 Is Down-Regulated by Hypovirus Infection and Required for Virulence and Female Fertility of the Chestnut Blight Fungus Cryphonectria parasitica

2006 ◽  
Vol 6 (2) ◽  
pp. 235-244 ◽  
Author(s):  
Fuyou Deng ◽  
Todd D. Allen ◽  
Donald L. Nuss
Keyword(s):  
Transcription Factor ◽  
Expressed Sequence Tag ◽  
Substantial Reduction ◽  
Cryphonectria Parasitica ◽  
Female Fertility ◽  
Chestnut Blight ◽  
Cellular Transcription Factor ◽  
Chestnut Blight Fungus ◽  
High Level

ABSTRACT A putative homologue of the Saccharomyces cerevisiae Ste12 transcription factor was identified in a series of expressed sequence tag-based microarray analyses as being down-regulated in strains of the chestnut blight fungus, Cryphonectria parasitica, infected by virulence-attenuating hypoviruses. Cloning of the corresponding gene, cpst12, confirmed a high level of similarity to Ste12 homologues of other filamentous fungi. Disruption of cpst12 resulted in no alterations in in vitro growth characteristics or colony morphology and an increase in the production of asexual spores, indicating that CpST12 is dispensable for vegetative growth and conidiation on artificial medium. However, the disruption mutants showed a very substantial reduction in virulence on chestnut tissue and a complete loss of female fertility, two symptoms normally conferred by hypovirus infection. Both virulence and female fertility were restored by complementation with the wild-type cpst12 gene. Analysis of transcriptional changes caused by cpst12 gene disruption with a custom C. parastica cDNA microaray chip identified 152 responsive genes. A significant number of these putative CpST12-regulated genes were also responsive to hypovirus infection. Thus, cpst12 encodes a cellular transcription factor, CpST12, that is down-regulated by hypovirus infection and required for female fertility, virulence and regulated expression of a subset of hypovirus responsive host genes.

scholarly journals Isolation and molecular characterization of a Lotus japonicus R2R3-MYB subgroup 7 transcription factor gene

2017 ◽  
Vol 34 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Satoru Kunihiro ◽  
Daigo Tanabe ◽  
Yuiko Niwa ◽  
Keisuke Kitamura ◽  
Jun Abe ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Molecular Characterization ◽  
Lotus Japonicus ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
R2r3 Myb

scholarly journals Characterization of intracellular free Ca2+ movements in neural progenitor cells derived from ES cells transfected with MASH1 transcription factor gene

Ensho Saisei ◽  
2005 ◽  
Vol 25 (5) ◽  
pp. 452-460 ◽  
Author(s):  
Michiko Ide ◽  
Yuji Ueda ◽  
Kenji Watanabe ◽  
Manae S. Kurokawa ◽  
Hideshi Yoshikawa ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Es Cells ◽  
Neural Progenitor ◽  
Transcription Factor Gene ◽  
Factor Gene

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

2008 ◽  
Vol 67 (1-2) ◽  
pp. 169-181 ◽  
Author(s):  
Honghong Hu ◽  
Jun You ◽  
Yujie Fang ◽  
Xiaoyi Zhu ◽  
Zhuyun Qi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Tolerance ◽  
Transcription Factor Gene ◽  
Factor Gene

scholarly journals Characterization of Hypovirus-Derived Small RNAs Generated in the Chestnut Blight Fungus by an Inducible DCL-2-Dependent Pathway

2008 ◽  
Vol 82 (6) ◽  
pp. 2613-2619 ◽  
Author(s):  
Xuemin Zhang ◽  
Gert C. Segers ◽  
Qihong Sun ◽  
Fuyou Deng ◽  
Donald L. Nuss
Keyword(s):  
Small Rnas ◽  
Rna Silencing ◽  
Cryphonectria Parasitica ◽  
Chestnut Blight ◽  
Fungal Host ◽  
Chestnut Blight Fungus ◽  
Mutant Strains ◽  
Suppressor Of Rna Silencing ◽  
Dependent Pathway

ABSTRACT The disruption of one of two dicer genes, dcl-2, of the chestnut blight fungus Cryphonectria parasitica was recently shown to increase susceptibility to mycovirus infection (G. C. Segers, X. Zhang, F. Deng, Q. Sun, and D. L. Nuss, Proc. Natl. Acad. Sci. USA 104:12902-12906, 2007). We now report the accumulation of virus-derived small RNAs (vsRNAs) in hypovirus CHV1-EP713-infected wild-type and dicer gene dcl-1 mutant C. parasitica strains but not in hypovirus-infected dcl-2 mutant and dcl-1 dcl-2 double-mutant strains. The CHV1-EP713 vsRNAs were produced from both the positive and negative viral RNA strands at a ratio of 3:2 in a nonrandom distribution along the viral genome. We also show that C. parasitica responds to hypovirus and mycoreovirus infections with a significant increase (12- to 20-fold) in dcl-2 expression while the expression of dcl-1 is increased only modestly (2-fold). The expression of dcl-2 is further increased (∼35-fold) following infection with a hypovirus CHV1-EP713 mutant that lacks the p29 suppressor of RNA silencing. The combined results demonstrate the biogenesis of mycovirus-derived small RNAs in a fungal host through the action of a specific dicer gene, dcl-2. They also reveal that dcl-2 expression is significantly induced in response to mycovirus infection by a mechanism that appears to be repressed by the hypovirus-encoded p29 suppressor of RNA silencing.

scholarly journals Functional Characterization of TaFUSCA3, a B3-Superfamily Transcription Factor Gene in the Wheat

2017 ◽  
Vol 8 ◽  
Author(s):  
Fusheng Sun ◽  
Xiyan Liu ◽  
Qiuhui Wei ◽  
Jiannan Liu ◽  
Tianxiang Yang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Functional Characterization ◽  
Transcription Factor Gene ◽  
Factor Gene
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