Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Monoclonal Antibodies as Tools for the Subgrouping of Escherichia coli Lipopolysaccharide O18 and O23 Antigens

1986 ◽  
Vol 54 (3) ◽  
pp. 931-931
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibodies ◽  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Dodecyl Sulfate ◽  
Escherichia Coli Lipopolysaccharide

scholarly journals mraY Is an Essential Gene for Cell Growth in Escherichia coli

1998 ◽  
Vol 180 (23) ◽  
pp. 6429-6432 ◽  
Author(s):  
David S. Boyle ◽  
William D. Donachie
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Essential Gene ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Dodecyl Sulfate

ABSTRACT The synthesis of the murein precursor lipid I is performed by MraY. We have shown that mraY is an essential gene for cell growth. Cells depleted of MraY first swell and then lyse. The expression of mraY DNA in vitro produces a 40-kDa polypeptide detectable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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