scholarly journals Cloning and expression in Escherichia coli of the perfringolysin O (theta-toxin) gene from Clostridium perfringens and characterization of the gene product.

1988 ◽  
Vol 56 (12) ◽  
pp. 3228-3234 ◽  
Author(s):  
R K Tweten
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Clostridium Perfringens ◽  
Cloning And Expression ◽  
Toxin Gene ◽  
Perfringolysin O ◽  
Expression In Escherichia Coli

scholarly journals Perfringolysin O Expression in Clostridium perfringens Is Independent of the Upstream pfoR Gene

2002 ◽  
Vol 184 (7) ◽  
pp. 2034-2038 ◽  
Author(s):  
Milena M. Awad ◽  
Julian I. Rood
Keyword(s):  
Escherichia Coli ◽  
Homologous Recombination ◽  
Gene Product ◽  
Structural Gene ◽  
Clostridium Perfringens ◽  
Deletion Mutant ◽  
Gas Gangrene ◽  
Alpha Toxin ◽  
Clostridial Myonecrosis ◽  
Perfringolysin O

ABSTRACT The pathogenesis of Clostridium perfringens-mediated gas gangrene or clostridial myonecrosis involves the extracellular toxins alpha-toxin and perfringolysin O. Previous studies (T. Shimizu, A. Okabe, J. Minami, and H. Hayashi, Infect. Immun. 59:137-142, 1991) carried out with Escherichia coli suggested that the perfringolysin O structural gene, pfoA, was positively regulated by the product of the upstream pfoR gene. In an attempt to confirm this hypothesis in C. perfringens, a pfoR-pfoA deletion mutant was complemented with isogenic pfoA+ shuttle plasmids that varied only in their ability to encode an intact pfoR gene. No difference in the ability to produce perfringolysin O was observed for C. perfringens strains carrying these plasmids. In addition, chromosomal pfoR mutants were constructed by homologous recombination in C. perfringens. Again no difference in perfringolysin O activity was observed. Since it was not possible to alter perfringolysin O expression by mutation of pfoR, it was concluded that the pfoR gene product is unlikely to have a role in the regulation of pfoA expression in C. perfringens.

scholarly journals Cloning and expression of endo-.BETA.-1,3-glucanase gene from Flavobacterium dormitator in Escherichia coli and characterization of the gene product.

1990 ◽  
Vol 54 (10) ◽  
pp. 2675-2680
Author(s):  
Shinji NAGATA ◽  
Isafumi MARU ◽  
Fumiaki ISHIHARA ◽  
Haruo MISONO ◽  
Susumu NAGASAKI
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Cloning And Expression
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