scholarly journals The conserved 18,000-molecular-weight outer membrane protein of Haemophilus ducreyi has homology to PAL.

1996 ◽  
Vol 64 (6) ◽  
pp. 1950-1955 ◽  
Author(s):  
S M Spinola ◽  
T J Hiltke ◽  
K Fortney ◽  
K L Shanks
Keyword(s):  
Molecular Weight ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Haemophilus Ducreyi

scholarly journals Localization of Haemophilus ducreyi at the Pustular Stage of Disease in the Human Model of Infection

2000 ◽  
Vol 68 (4) ◽  
pp. 2309-2314 ◽  
Author(s):  
Margaret E. Bauer ◽  
Stanley M. Spinola
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Human Subjects ◽  
Haemophilus Ducreyi ◽  
Human Model ◽  
Stage Of Disease ◽  
The One ◽  
Secondary Antibodies

ABSTRACT To localize Haemophilus ducreyi in vivo, human subjects were experimentally infected with H. ducreyi until they developed a painful pustule or for 14 days. Lesions were biopsied, and biopsy samples were fixed in 4% paraformaldehyde, and cryosectioned. Sections were stained with polyclonal anti-H. ducreyi antiserum or H. ducreyi-specific monoclonal antibodies (MAbs) and fluorescently tagged secondary antibodies and examined by confocal microscopy. We identified H. ducreyi in 16 of 18 pustules but did not detect bacteria in the one papule examined. H. ducreyi was observed as individual cells and in clumps or chains. Staining with MAbs 2D8, 5C9, 3B9, 2C7, and 9D12 demonstrated that H. ducreyi expresses the major pilus subunit, FtpA, the 28-kDa outer membrane protein Hlp, the 18-kDa outer membrane protein PAL, and the major outer membrane protein (MOMP) or OmpA2 in vivo. By dual staining with polyclonal anti-H. ducreyi antiserum and MAbs that recognize human skin components, we observed bacteria within the neutrophilic infiltrates of all positively staining pustules and in the dermis of 10 of 16 pustules. We were unable to detect bacteria associated with keratinocytes in the samples examined. The data suggest that H. ducreyi is found primarily in association with neutrophils and in the dermis at the pustular stage of disease in the human model of infection.

scholarly journals Chemical and Immunological Characterization of a Low Molecular Weight Outer Membrane Protein ofSalmonella typhi

1998 ◽  
Vol 42 (8) ◽  
pp. 521-526 ◽  
Author(s):  
Carlos Maurício De Andrade ◽  
Antônio Gomes Pinto Ferreira ◽  
Joana D'Arc Cardoso Da Silva ◽  
Hilton Jorge Nascimento ◽  
José Godinho Da Silva Junior
Keyword(s):  
Molecular Weight ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Low Molecular Weight ◽  
Immunological Characterization

scholarly journals A hemoglobin-binding outer membrane protein is involved in virulence expression by Haemophilus ducreyi in an animal model.

1996 ◽  
Vol 64 (5) ◽  
pp. 1724-1735 ◽  
Author(s):  
M K Stevens ◽  
S Porcella ◽  
J Klesney-Tait ◽  
S Lumbley ◽  
S E Thomas ◽  
...  
Keyword(s):  
Animal Model ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Haemophilus Ducreyi ◽  
Hemoglobin Binding
Sign in / Sign up

Export Citation Format

Share Document