Carotid artery velocity time integral and corrected flow time measured by a wearable Doppler ultrasound detect stroke volume rise from simulated hemorrhage to transfusion

Objective Doppler ultrasonography of the common carotid artery is used to infer stroke volume change and a wearable Doppler ultrasound has been designed to improve this workflow. Previously, in a human model of hemorrhage and resuscitation comprising approximately 50,000 cardiac cycles, we found a strong, linear correlation between changing stroke volume, and measures from the carotid Doppler signal, however, optimal Doppler thresholds for detecting a 10% stroke volume change were not reported. In this Research Note, we present these thresholds, their sensitivities, specificities and areas under their receiver operator curves (AUROC). Results Augmentation of carotid artery maximum velocity time integral and corrected flowtime by 18% and 4%, respectively, accurately captured 10% stroke volume rise. The sensitivity and specificity for these thresholds were identical at 89% and 100%. These data are similar to previous investigations in healthy volunteers monitored by the wearable ultrasound.

Rostral Hypothalamic Differentiation With Minimal Exogenous Signals in Human Naive Induced Pluripotent Stem Cells

Familial neurohypophyseal diabetes insipidus (FNDI) is a degenerative disease of vasopressin (AVP) neurons. Studies in mouse in vivo models indicate that accumulation of mutant AVP prehormone is associated with FNDI pathology. However, studying human FNDI pathology in vivo is technically challenging. Therefore, an in vitro human model needs to be developed. When exogenous signals are minimized in the early phase of differentiation in vitro, mouse embryonic stem cells (ESCs) differentiate into AVP neurons, whereas human ESCs/induced pluripotent stem cells (iPSCs) die. Human ES/iPSCs are generally more similar to mouse epiblast stem cells compared to mouse ESCs, which are termed as primed and naive, respectively. In this study, we converted human FNDI-specific iPSCs from primed to naive cells, and found improved cell survival under minimal exogenous signals and differentiation into rostral hypothalamic organoids. Overall, this method provides a simple and straightforward differentiation direction, which may improve the efficiency of hypothalamic differentiation.

Human Body Simulation Within a Hybrid Operating Method for a Safe and Efficient Human-Robot Collaboration

The planning and integration of production systems with a direct human-robot collaboration (HRC) is still associated with various technical challenges. This applies especially to the realization of the operation methods speed and separation monitoring (SSM) as well as power and force limiting (PFL). Due to the limited consideration of the human motion behaviour, the required dynamic separation distance in SSM is frequently oversized in practice. The main consequences are wasted space as well as cycle time and performance losses within the corresponding HRC application. In PFL a physical contact between the operator and robot is permissible, taking into account specified biomechanical thresholds. However, there is still a lack of suitable use-cases since the maximum permissible speeds are on a very low level. Moreover some thresholds regarding the transient contact case are still non-applicable for critical body areas (e.g. temple, middle of forehead). The study of this paper is related to a kinematic state determination of the human operator within a new hybrid collaborative operation. In this method the SSM type is extended regarding the description of the operator and coupled with the two-body contact model of the PFL. Using a planning and simulation tool for HRC, the kinematic states of different body regions are derived from an integrated and parameterized digital human model. Afterwards, these body regions are mapped to the characteristic body areas of the ISO/TS 15066, whereby the resulting information will be applied in an adaptive robot speed control. The performance of the presented concept will be evaluated using an exemplary simulated HRC scenario.

Numerical biomechanics modelling of indirect mitral annuloplasty treatments for functional mitral regurgitation

Mitral valve regurgitation (MR) is a common valvular heart disease where an improper closure leads to leakage from the left ventricle into the left atrium. There is a need for less-invasive treatments such as percutaneous repairs for a large inoperable patient population. The aim of this study is to compare several indirect mitral annuloplasty (IMA) percutaneous repair techniques by finite-element analyses. Two types of generic IMA devices were considered, based on coronary sinus vein shortening (IMA-CS) to reduce the annulus perimeter and based on shortening of the anterior–posterior diameter (IMA-AP). The disease, its treatments, and the heart function post-repair were modelled by modifying the living heart human model (Dassault Systèmes). A functional MR pathology that represents ischaemic MR was generated and the IMA treatments were simulated in it, followed by heart function simulations with the devices and leakage quantification from blood flow simulations. All treatments were able to reduce leakage, the IMA-AP device achieved better sealing, and there was a correlation between the IMA-CS device length and the reduction in leakage. The results of this study can help in bringing IMA-AP to market, expanding the use of IMA devices, and optimizing future designs of such devices.

Evaluation of Peripheral Electrostimulation Thresholds in Human Model for Uniform Magnetic Field Exposure

The external field strength according to the international guidelines and standards for human protection are derived to prevent peripheral nerve system pain at frequencies from 300–750 Hz to 1 MHz. In this frequency range, the stimulation is attributable to axon electrostimulation. One limitation in the current international guidelines is the lack of respective stimulation thresholds in the brain and peripheral nervous system from in vivo human measurements over a wide frequency range. This study investigates peripheral stimulation thresholds using a multi-scale computation based on a human anatomical model for uniform exposure. The nerve parameters are first adjusted from the measured data to fit the peripheral nerve in the trunk. From the parameters, the external magnetic field strength to stimulate the nerve was estimated. Here, the conservativeness of protection limits of the international guidelines and standards for peripheral stimulation was confirmed. The results showed a margin factor of 4–6 and 10–24 times between internal and external protection limits of Institute of Electrical and Electronics Engineers standard (IEEE C95.1) and International Commission on Non-Ionizing Radiation Protection guidelines, with the computed pain thresholds.

Research on Virtual Display of Wetsuit Based on CLO3D

In order to overcome the problems of low signal-to-noise ratio in the information output interface and long time for information synthesis in the traditional virtual display method of clothing, a CLO3D-based virtual display method for wetsuit is designed in this study. The proposed method works as follows. Firstly, it analyzes the categories and functional characteristics of the wetsuit and the virtual display process of the CLO3D software. In the second step, the design of the proposed method for the process of data collection and fusion of the wetsuit design is made. In the subsequent steps, human model is established, designs are made for the style and modeling, simulation is made for the pattern and color of the wetsuit fabric, and dynamic display is made. Experimental results show that the signal-to-noise ratio (SNR) of the information output interface of the proposed method is above 75 dB, and the maximum SNR can reach 80.5 dB, and the information synthesis time varies between 32 min and 47 min, indicating that the proposed method is more efficient and effective.

COPING ENHANCEMENT PROGAM FOR THE MOTHERS OF SPECIAL CHILDREN- A PRELIMINARY STUDY

Background: Caring for a family member with a special challenging problem is a signicant chronic stressor in humans.In fact, caregiver stress is currently seen as a human model of chronic stress in the stress literature. As a need based interventions with mothers can help them reduce their stress and increase their coping levels, this study was conducted on the effectiveness of coping enhancement program for the mothers of special children. Objective:The overall objective of this study was to establish whether the Coping enhancement program could be utilized when the population of interest is caregivers of special children with psychiatric, behavioral or emotional disorders. Study design:A survey cum quasi experimental, one group pretest post test design was used for this study. Materials and methods:The present study was conducted in three phases- Assessment of stress, coping level and coping factors of mothers of special children, development of a coping enhancement programme and administering the coping enhancement programme to mothers. The effectiveness of the program was judged by the difference between the pretest and post test score. The population selected for the study were the mothers of the special children admitted in special schools of Puducherry Union Territory. Results: The coping enhancement programme was found to be signicantly effective among mothers handling special children in coping with their stress and thus it may result in enhancing the quality of life of the special children.

Tabula Microcebus: A transcriptomic cell atlas of mouse lemur, an emerging primate model organism

Mouse lemurs are the smallest, fastest reproducing, and among the most abundant primates, and an emerging model organism for primate biology, behavior, health and conservation. Although much has been learned about their physiology and their Madagascar ecology and phylogeny, little is known about their cellular and molecular biology. Here we used droplet- and plate-based single cell RNA-sequencing to profile 226,000 cells from 27 mouse lemur organs and tissues opportunistically procured from four donors clinically and histologically characterized. Using computational cell clustering, integration, and expert cell annotation, we defined and biologically organized over 750 mouse lemur molecular cell types and their full gene expression profiles. These include cognates of most classical human cell types, including stem and progenitor cells, and the developmental programs for spermatogenesis, hematopoiesis, and other adult tissues. We also described dozens of previously unidentified or sparsely characterized cell types and subtypes. We globally compared cell type expression profiles to define the molecular relationships of cell types across the body, and explored primate cell type evolution by comparing mouse lemur cell profiles to those of the homologous cells in human and mouse. This revealed cell type specific patterns of primate cell specialization even within a single tissue compartment, as well as many cell types for which lemur provides a better human model than mouse. The atlas provides a cellular and molecular foundation for studying this primate model organism, and establishes a general approach for other emerging model organisms.

Visible Human Project® female surface based computational phantom (Nelly) for radio-frequency safety evaluation in MRI coils

Quantitative modeling of specific absorption rate and temperature rise within the human body during 1.5 T and 3 T MRI scans is of clinical significance to ensure patient safety. This work presents justification, via validation and comparison, of the potential use of the Visible Human Project (VHP) derived Computer Aided Design (CAD) female full body computational human model for non-clinical assessment of female patients of age 50–65 years with a BMI of 30–36 during 1.5 T and 3 T based MRI procedures. The initial segmentation validation and four different application examples have been identified and used to compare to numerical simulation results obtained using VHP Female computational human model under the same or similar conditions. The first application example provides a simulation-to-simulation validation while the latter three application examples compare with measured experimental data. Given the same or similar coil settings, the computational human model generates meaningful results for SAR, B1 field, and temperature rise when used in conjunction with the 1.5 T birdcage MRI coils or at higher frequencies corresponding to 3 T MRI. Notably, the deviation in temperature rise from experiment did not exceed 2.75° C for three different heating scenarios considered in the study with relative deviations of 10%, 25%, and 20%. This study provides a reasonably systematic validation and comparison of the VHP-Female CAD v.3.0–5.0 surface-based computational human model starting with the segmentation validation and following four different application examples.