scholarly journals Variation in the In Vitro Migration of Sensitized Guinea Pig Peritoneal Exudate Cells in the Presence of Coccidioidin

1974 ◽  
Vol 9 (2) ◽  
pp. 416-418 ◽  
Author(s):  
Ferne Zabezensky ◽  
William T. Northey
Keyword(s):  
Guinea Pig ◽  
Peritoneal Exudate ◽  
Peritoneal Exudate Cells

scholarly journals Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. II. T lymphocyte-mediated cytolysis.

1980 ◽  
Vol 152 (5) ◽  
pp. 1184-1193 ◽  
Author(s):  
T Boon ◽  
J Van Snick ◽  
A Van Pel ◽  
C Uyttenhove ◽  
M Marchand
Keyword(s):  
Tumor Cell ◽  
T Lymphocyte ◽  
Peritoneal Exudate ◽  
Antigenic Specificity ◽  
Spleen Cells ◽  
Peritoneal Exudate Cells ◽  
Specific Antigens

Tumor cell variants that were rejected by syngeneic mice (tum-) were obtained from mastocytoma P815 by mutagenesis (as described in the accompanying report (13). A considerable T lymphocyte-mediated lysis was observed upon incubation of these tum- variants with peritoneal exudate cells collected a few days after an intraperitoneal challenge of immune animals. Spleen cells from these animals were cytolytic after stimulation in vitro with the immunizing variant. New antigens, absent from the original P815 tum+ cells, were detected on 15 of the 21 tum- variants that were tested. All these antigens appeared to be different. No new antigen was detected on any of 10 mutagenized P815 clones that had retained their ability to form tumors. We compared the evidence obtained in vivo and in vitro for the presence of specific antigens on five tum- variants. Three variants were shown both in vivo and in vitro to carry an individual antigen. One showed no specificity either in vivo or in vitro. However, for one variant, no specificity was observed in vivo, although cytolysis tests demonstrated the existence of a singular antigenic specificity.

In vitro antiphagocytic effect of Melia azedarach leaf extracts on mouse peritoneal exudate cells

1994 ◽  
Vol 43 (2) ◽  
pp. 135-140 ◽  
Author(s):  
M.C. Courrèges ◽  
F. Benencia ◽  
C.E. Coto ◽  
E.J. Massouh ◽  
F.C. Coulombié
Keyword(s):  
Peritoneal Exudate ◽  
Melia Azedarach ◽  
Leaf Extracts ◽  
Peritoneal Exudate Cells

A Technique for Sequential Examination of in Vitro Macrophage-Tumor Interaction using Lm, Sem and Tem

1976 ◽  
Vol 34 ◽  
pp. 350-351
Author(s):  
C. Bucana ◽  
B. Hobbs ◽  
L. C. Hoyer ◽  
M. G. Hanna
Keyword(s):  
Guinea Pig ◽  
Tumor Cell ◽  
Tumor Immunology ◽  
Time Lapse ◽  
Cell Interaction ◽  
Peritoneal Exudate Cells ◽  
Cytotoxicity Assays ◽  
Sem And Tem

A sequential examination of macrophage-tumor cell interaction in vitro, using time-lapse cinematography followed by SEM and TEM of the same cells, is a valuable asset in the interpretation of in vivo and in vitro cell-mediated cytotoxicity assays that are widely used in tumor immunology. Previous reports on sequential examination of biological specimens involved large tissue blocks. This report describes a technique that allows sequential examination of cell - cell interaction using three modes of microscopy.Guinea pig hepatoma (L-10) cells were plated sparsely on 25mm glass coverslips 24hr before adding guinea pig peritoneal exudate cells (PEC). PEC were allowed to settle for 1 l/2hr; the coverslips were then transferred to a Dvorak-Stotler chamber and examined with a Zeiss photomicroscope III.

scholarly journals Independent populations of primed F1 guinea pig T lymphocytes respond to antigen-pulsed parental peritoneal exudate cells.

1977 ◽  
Vol 145 (3) ◽  
pp. 618-630 ◽  
Author(s):  
W E Paul ◽  
E M Shevach ◽  
S Pickeral ◽  
D W Thomas ◽  
A S Rosenthal
Keyword(s):  
T Lymphocytes ◽  
Negative Selection ◽  
Suppressor Cells ◽  
Peritoneal Exudate ◽  
Proliferating Cells ◽  
F1 Hybrid ◽  
Peritoneal Exudate Cells ◽  
Purified Protein Derivative ◽  
Initial Culture

Thymus-dependent (T) lymphocytes from (2 x 13)F1 hybrid guinea pigs immunized to ovalbumin (OVA) in complete Freund's adjuvant can be stimulated to proliferate in vitro by antigen-pulsed peritoneal exudate cells (PECs) derived from either strain 2 or strain 13 donors. In this communication, we show that the population of primed F1 T lymphocytes which can be activated by antigen-pulsed strain 2 PECs is largely independent of the population of cells that can be activated by antigen-pulsed strain 13 PECs. This was demonstrated by both positive and negative selection procedures. In the former, T lymphocytes from OVA-primed (2 x 13)F1 donors were enriched by initial culture with OVA-pulsed strain 2 or strain 13 PECs for 1 wk. Cells selected by culture with OVA-pulsed strain 2 PECs responded well to OVA-pulsed strain 2 PECs and poorly to OVA-pulsed strain 13 PECs. If positive selection had been carried out with OVA-pulsed strain 13 PECs, the selected F1 T cells responded well to OVA-pulsed 13 PECs and poorly to OVA-pulsed 2 PECs. Negative selection was achieved by short term culture with antigen-pulsed PECs and by eliminating proliferating cells by treatment with bromodeoxyuridine and light. This procedure demonstrated that the population of primed F1 T lymphocytes which are responsive to OVA or to purified protein derivative of tuberculin can be divided into subpopulations uniquely responsive to antigen on either strain 2 or strain 13 PECs. Evidence was presented to indicate that this selective responsiveness was not the result of the action of alloantigen-specific suppressor cells. The results are considered in terms of current concepts of the genetic and molecular regulation of the interaction of PECs and T lymphocytes.

scholarly journals THE IN VITRO DESENSITIZATION OF SENSITIVE CELLS BY TRYPSIN

1964 ◽  
Vol 120 (6) ◽  
pp. 1189-1200 ◽  
Author(s):  
John R. David ◽  
H. S. Lawrence ◽  
L. Thomas
Keyword(s):  
Trypsin Inhibitor ◽  
Specific Antigen ◽  
Peritoneal Exudate ◽  
Soybean Trypsin Inhibitor ◽  
Delayed Hypersensitivity ◽  
Peritoneal Exudate Cells ◽  
Peritoneal Cells

Peritoneal exudate cells from animals exhibiting delayed hypersensitivity are inhibited from migrating in vitro by specific antigen. This inhibition is completely abolished by pretreatment of the sensitive cells with trypsin. The action of trypsin is prevented by soybean trypsin inhibitor. The results of experiments with mixtures of normal and sensitive cells suggest that trypsin alters an immunologic capacity of the sensitive cells. Trypsinized sensitive cells are capable of passively transferring delayed hypersensitivity and peritoneal cells taken from recipient animals are inhibited from migrating in vitro by specific antigen. These results suggest that the cells rapidly resynthesize the material removed by trypsin. The possible nature of the material removed by trypsin is discussed.

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