scholarly journals New Transport Medium for Cultural Recovery of Helicobacter pylori

2014 ◽  
Vol 52 (12) ◽  
pp. 4325-4329 ◽  
Author(s):  
L. Cellini ◽  
E. Di Campli ◽  
S. Di Bartolomeo ◽  
L. J. Bessa ◽  
M. Baffoni ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Transport Medium ◽  
Cultural Recovery

Methodology and Transport Medium for Collection of Helicobacter pylori on a String Test in Remote Locations

Helicobacter ◽  
2005 ◽  
Vol 10 (6) ◽  
pp. 630-634 ◽  
Author(s):  
Helen M. Windsor ◽  
Emmanuel A. Abioye-Kuteyi ◽  
Barry J. Marshall
Keyword(s):  
Helicobacter Pylori ◽  
Transport Medium ◽  
Remote Locations ◽  
String Test

scholarly journals Evaluation of a New Transport Medium (HP Medium) for Helicobacter pylori

1995 ◽  
Vol 69 (1) ◽  
pp. 123-124
Author(s):  
Intetsu KOBAYASHI ◽  
Miyuki HASEGAWA ◽  
Toshio FUJIOKA ◽  
Masaru NASU
Keyword(s):  
Helicobacter Pylori ◽  
Transport Medium

scholarly journals Effect of transport medium and transportation time on culture of Helicobacter pylori from gastric biopsy specimens.

1993 ◽  
Vol 46 (6) ◽  
pp. 561-563 ◽  
Author(s):  
R A Veenendaal ◽  
A T Lichtendahl-Bernards ◽  
A S Pena ◽  
H P Endtz ◽  
C P van Boven ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Transportation Time ◽  
Transport Medium ◽  
Biopsy Specimens

scholarly journals Evaluation of a Selective Transport Medium for Gastric Biopsy Specimens To Be Cultured for Helicobacter pylori

1998 ◽  
Vol 36 (10) ◽  
pp. 3048-3050 ◽  
Author(s):  
L. K. Siu ◽  
W. K. Leung ◽  
A. F. B. Cheng ◽  
J. Y. Sung ◽  
T. K. W. Ling ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Horse Serum ◽  
Brain Heart Infusion ◽  
Selective Medium ◽  
Gastric Biopsy ◽  
Detection Rates ◽  
Transport Medium ◽  
Selective Transport ◽  
Biopsy Specimens ◽  
H Pylori

Since the means of culturing Helicobacter pylori may not be available in some laboratories, prolonging the survival of this organism during transportation is a major concern in terms of improving detection rates. A selective transport medium was evaluated for the preservation of H. pylori from 254 gastric biopsy specimens collected from a rural area in China where culturing is not feasible. Gastric biopsy specimens were inoculated in sterile broth consisting of brain heart infusion (BHI) broth, horse serum, and yeast extract supplemented with vancomycin, amphotericin B, and nalidixic acid (VAN). Of the 254 biopsy specimens, 238 were identified by histology to haveH. pylori infection. Total rates of recovery ofH. pylori from the H. pylori-positive gastric biopsy specimens stored in the BHI-VAN broth ranged from 76 to 46% after storage of specimens for 5 to 9 days. In conclusion, the selective medium is useful for prolonging the survival of H. pylori in gastric biopsy specimens for which immediate culture is not feasible.

The rod-to-coccoid body transformation in cultures of Helicobacter pylori

1990 ◽  
Vol 48 (3) ◽  
pp. 626-627
Author(s):  
A. R. Crooker ◽  
W. G. Kraft ◽  
T. L. Beard ◽  
M. C. Myers
Keyword(s):  
Helicobacter Pylori ◽  
Growth Cycle ◽  
Negative Bacterium ◽  
Body Formation ◽  
Coccoid Form ◽  
Spiral Form ◽  
Prolonged Culture ◽  
H Pylori ◽  
Upper Gastrointestinal

Helicobacter pylori is a microaerophilic, gram-negative bacterium found in the upper gastrointestinal tract of humans. There is strong evidence that H. pylori is important in the etiology of gastritis; the bacterium may also be a major predisposing cause of peptic ulceration. On the gastric mucosa, the organism exists as a spiral form with one to seven sheathed flagella at one (usually) or both poles. Short spirals were seen in the first successful culture of the organism in 1983. In 1984, Marshall and Warren reported a coccoid form in older cultures. Since that time, other workers have observed rod and coccal forms in vitro; coccoid forms predominate in cultures 3-7 days old. We sought to examine the growth cycle of H. pylori in prolonged culture and the mode of coccoid body formation.

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