Microtiter ganglioside enzyme-linked immunosorbent assay for vibrio and Escherichia coli heat-labile enterotoxins and antitoxin.

1980 ◽  
Vol 11 (1) ◽  
pp. 35-40 ◽  
Author(s):  
D A Sack ◽  
S Huda ◽  
P K Neogi ◽  
R R Daniel ◽  
W M Spira
Keyword(s):  
Escherichia Coli ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Heat Labile

scholarly journals Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin

1977 ◽  
Vol 6 (5) ◽  
pp. 439-444 ◽  
Author(s):  
R H Yolken ◽  
H B Greenberg ◽  
M H Merson ◽  
R B Sack ◽  
A Z Kapikian
Keyword(s):  
Escherichia Coli ◽  
Immunosorbent Assay ◽  
Radioactive Isotope ◽  
Enzyme Linked Immunosorbent Assay ◽  
Adrenal Cell ◽  
Elisa System ◽  
Culture Techniques ◽  
E Coli ◽  
Heat Labile ◽  
Tissue Culture Techniques

The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.

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