Detection of human immunoglobulins G and M antibodies to Rift Valley fever virus by enzyme-linked immunosorbent assay.

1984 ◽  
Vol 19 (2) ◽  
pp. 225-229 ◽  
Author(s):  
B Niklasson ◽  
C J Peters ◽  
M Grandien ◽  
O Wood
Keyword(s):  
Immunosorbent Assay ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Valley Fever ◽  
Human Immunoglobulins

scholarly journals Detection of Rift Valley Fever Virus Lineage H from South Africa through Syndromic Sentinel Surveillance Network in Senegal

2021 ◽  
Author(s):  
Ndeye Sakha Bob ◽  
Mamadou Aliou Barry ◽  
Moussa Moise Diagne ◽  
Martin Faye ◽  
Marie Henriette Dior Ndione ◽  
...  
Keyword(s):  
South Africa ◽  
Rift Valley ◽  
Surveillance System ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Surveillance Network ◽  
Valley Fever

Abstract Background Rift Valley fever virus (RVFV) is an arbovirus that causes epizootics and epidemics among livestock population and humans. Our surveillance system has revealed multiple emergences and re-emergences of RVFV in West Africa over the last decade. Methods In Senegal a sentinel syndromic surveillance network (4S) has been implemented since 2011. Samples from human suspected arbovirus infection in 4S sentinel sites were sent to Institut Pasteur de Dakar (IPD) where arbovirus diagnosis by enzyme-linked immunosorbent assay (ELISA), real-time reverse transcription polymerase chain reaction (RT-PCR), and virus isolation were performed. Overall, IPD has received a total of 1,149 samples from arboviral suspected patients through the 4S network from January to December 2020. These samples were screened for seven arboviruses including RVFV. Whole genome sequencing of positive RVFV samples by RT-PCR were performed using Illumina Miseq platform followed by genome assembly. Phylogenetic analysis were performed using MEGA X. Results Out of the 1,149 arbovirus suspected cases, four RVFV positive samples were detected with RT-PCR while five RVFV positive samples were detected by ELISA. Complete genome sequences were obtained for three strains among the four positive samples by RT-PCR. Phylogenetic analyses indicated an emergence of a virus first described in South Africa during a major outbreak. Conclusion Strong surveillance system allowed the detection of RVFV outbreak in Senegal in 2020. The obtained genomes clustered with strains from South Africa belonging to lineage H. This calls for an implementation of a strong surveillance system in wild animals, humans, and livestock simultaneously in all African Countrries.

scholarly journals Large-Scale International Validation of an Indirect ELISA Based on Recombinant Nucleocapsid Protein of Rift Valley Fever Virus for the Detection of IgG Antibody in Domestic Ruminants

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1651
Author(s):  
Janusz T. Pawęska ◽  
Petrus Jansen van Vuren ◽  
Veerle Msimang ◽  
Modu Moustapha Lô ◽  
Yaya Thiongane ◽  
...  
Keyword(s):  
Rift Valley ◽  
Nucleocapsid Protein ◽  
Rift Valley Fever ◽  
Large Scale ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Valley Fever ◽  
Recombinant Nucleocapsid Protein

Diagnostic performance of an indirect enzyme-linked immunosorbent assay (I-ELISA) based on a recombinant nucleocapsid protein (rNP) of the Rift Valley fever virus (RVFV) was validated for the detection of the IgG antibody in sheep (n = 3367), goat (n = 2632), and cattle (n = 3819) sera. Validation data sets were dichotomized according to the results of a virus neutralization test in sera obtained from RVF-endemic (Burkina Faso, Democratic Republic of Congo, Mozambique, Senegal, Uganda, and Yemen) and RVF-free countries (France, Poland, and the USA). Cut-off values were defined using the two-graph receiver operating characteristic analysis. Estimates of the diagnostic specificity of the RVFV rNP I-ELISA in animals from RVF-endemic countries ranged from 98.6% (cattle) to 99.5% (sheep) while in those originating from RVF-free countries, they ranged from 97.7% (sheep) to 98.1% (goats). Estimates of the diagnostic sensitivity in ruminants from RVF-endemic countries ranged from 90.7% (cattle) to 100% (goats). The results of this large-scale international validation study demonstrate the high diagnostic accuracy of the RVFV rNP I-ELISA. Standard incubation and inactivation procedures evaluated did not have an adverse effect on the detectable levels of the anti-RVFV IgG in ruminant sera and thus, together with recombinant antigen-based I-ELISA, provide a simple, safe, and robust diagnostic platform that can be automated and carried out outside expensive bio-containment facilities. These advantages are particularly important for less-resourced countries where there is a need to accelerate and improve RVF surveillance and research on epidemiology as well as to advance disease control measures.

Complement fixation reaction of Rift Valley fever virus

1950 ◽  
Vol 5 (5) ◽  
pp. 243-247
Author(s):  
Minoru MATSUMOTO ◽  
Saburo IWASA ◽  
Motosige ENDO
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Complement Fixation ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Fixation Reaction
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