scholarly journals Streptococcus pyogenes pharyngitis: characterization of strains by multilocus enzyme genotype, M and T protein serotype, and pyrogenic exotoxin gene probing.

1992 ◽  
Vol 30 (3) ◽  
pp. 600-603 ◽  
Author(s):  
J M Musser ◽  
B M Gray ◽  
P M Schlievert ◽  
M E Pichichero
Keyword(s):  
Streptococcus Pyogenes ◽  
Gene Probing

scholarly journals Genetic characterization of Streptococcus pyogenes emm 89 strains isolated in Japan from 2011 to 2019

2020 ◽  
Author(s):  
Yujiro Hirose ◽  
Masaya Yamaguchi ◽  
Norihiko Takemoto ◽  
Tohru Miyoshi-Akiyama ◽  
Tomoko Sumitomo ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Genetic Characterization ◽  
Streptococcal Toxic Shock Syndrome ◽  
Toxic Shock ◽  
Gene Encoding ◽  
Serum Opacity Factor ◽  
Genetic Features ◽  
Fibronectin Binding Protein

Streptococcal toxic shock syndrome (STSS) caused by Streptococcus pyogenes emm 89 strains has been increasing in several countries and reported to be linked with a recently emerged clade of emm89 strains, designated clade 3. In Japan, epidemiological and genetic information for emm89 strains remains elusive. In this study, we utilized emm89 strains isolated from both STSS (89 isolates) and non-STSS (72 isolates) infections in Japan from 2011 to 2019, and conducted whole-genome sequencing and comparative analysis, which resulted in classification of a large majority into clade 3 regardless of disease severity. In addition, STSS-associated genes and SNPs were found in clade 3 strains, including mutations of streptokinase (Ska), control of virulence sensor (CovS), serum opacity factor (SOF), sortase (SrtB), and fibronectin-binding protein F1 (PrtF1), and absence of the hylP1 gene encoding hyaluronidase. These findings provide insights into notable genetic features of emm89 strains.

Biophysical characterization of the interaction between heme and proteins responsible for heme transfer in Streptococcus pyogenes

2017 ◽  
Vol 493 (2) ◽  
pp. 1109-1114 ◽  
Author(s):  
Masato Hoshino ◽  
Makoto Nakakido ◽  
Satoru Nagatoishi ◽  
Chihiro Aikawa ◽  
Ichiro Nakagawa ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Biophysical Characterization ◽  
Heme Transfer

Molecular Characterization of Streptococcus pyogenes Causing Invasive Disease in Pediatric Population in Spain A 12-year Study

2019 ◽  
Vol 38 (12) ◽  
pp. 1168-1172
Author(s):  
Viviana Sánchez-Encinales ◽  
Guillermo Ludwig ◽  
Esther Tamayo ◽  
Jose Maria García-Arenzana ◽  
Carmen Muñoz-Almagro ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Streptococcus Pyogenes ◽  
Pediatric Population ◽  
Invasive Disease

Molecular epidemiology, antimicrobial susceptibility, and characterization of fluoroquinolone non-susceptible Streptococcus pyogenes in Japan

2020 ◽  
Vol 26 (3) ◽  
pp. 280-284 ◽  
Author(s):  
Kazuaki Tatara ◽  
Kenji Gotoh ◽  
Kiyohito Okumiya ◽  
Mariko Teramachi ◽  
Kazuhisa Ishimoto ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Streptococcus Pyogenes ◽  
Antimicrobial Susceptibility

scholarly journals Prophage exotoxins enhance colonization fitness in epidemic scarlet fever-causing Streptococcus pyogenes

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Stephan Brouwer ◽  
Timothy C. Barnett ◽  
Diane Ly ◽  
Katherine J. Kasper ◽  
David M. P. De Oliveira ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Scarlet Fever ◽  
Global Public Health ◽  
Nasopharyngeal Colonization ◽  
North East ◽  
Streptolysin O ◽  
Knockout Mutants ◽  
Group A ◽  
Bacterial Superantigen

Abstract The re-emergence of scarlet fever poses a new global public health threat. The capacity of North-East Asian serotype M12 (emm12) Streptococcus pyogenes (group A Streptococcus, GAS) to cause scarlet fever has been linked epidemiologically to the presence of novel prophages, including prophage ΦHKU.vir encoding the secreted superantigens SSA and SpeC and the DNase Spd1. Here, we report the molecular characterization of ΦHKU.vir-encoded exotoxins. We demonstrate that streptolysin O (SLO)-induced glutathione efflux from host cellular stores is a previously unappreciated GAS virulence mechanism that promotes SSA release and activity, representing the first description of a thiol-activated bacterial superantigen. Spd1 is required for resistance to neutrophil killing. Investigating single, double and triple isogenic knockout mutants of the ΦHKU.vir-encoded exotoxins, we find that SpeC and Spd1 act synergistically to facilitate nasopharyngeal colonization in a mouse model. These results offer insight into the pathogenesis of scarlet fever-causing GAS mediated by prophage ΦHKU.vir exotoxins.

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