scholarly journals Genetic characterization of Streptococcus pyogenes emm 89 strains isolated in Japan from 2011 to 2019

2020 ◽  
Author(s):  
Yujiro Hirose ◽  
Masaya Yamaguchi ◽  
Norihiko Takemoto ◽  
Tohru Miyoshi-Akiyama ◽  
Tomoko Sumitomo ◽  
...  

Streptococcal toxic shock syndrome (STSS) caused by Streptococcus pyogenes emm 89 strains has been increasing in several countries and reported to be linked with a recently emerged clade of emm89 strains, designated clade 3. In Japan, epidemiological and genetic information for emm89 strains remains elusive. In this study, we utilized emm89 strains isolated from both STSS (89 isolates) and non-STSS (72 isolates) infections in Japan from 2011 to 2019, and conducted whole-genome sequencing and comparative analysis, which resulted in classification of a large majority into clade 3 regardless of disease severity. In addition, STSS-associated genes and SNPs were found in clade 3 strains, including mutations of streptokinase (Ska), control of virulence sensor (CovS), serum opacity factor (SOF), sortase (SrtB), and fibronectin-binding protein F1 (PrtF1), and absence of the hylP1 gene encoding hyaluronidase. These findings provide insights into notable genetic features of emm89 strains.

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Tadayoshi Ikebe ◽  
Takayuki Matsumura ◽  
Hisako Nihonmatsu ◽  
Hitomi Ohya ◽  
Rumi Okuno ◽  
...  

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Tsuyoshi Sekizuka ◽  
Emina Nai ◽  
Tomohiro Yoshida ◽  
Shota Endo ◽  
Emi Hamajima ◽  
...  

2005 ◽  
Vol 37 (2) ◽  
pp. 85-89 ◽  
Author(s):  
J. Gooskens ◽  
A. J. De Neeling ◽  
R. J. Willems ◽  
J. W. Van 'T Wout ◽  
E. J. Kuijper

2008 ◽  
Vol 57 (11) ◽  
pp. 1354-1363 ◽  
Author(s):  
Bogdan Luca-Harari ◽  
Monica Straut ◽  
Silvia Cretoiu ◽  
Maria Surdeanu ◽  
Vasilica Ungureanu ◽  
...  

In 2002, the Romanian National Reference Laboratory was invited to join the Strep-EURO project to study invasive Streptococcus pyogenes infections. During 2003 and 2004, a total of 33 isolates recovered from invasive disease were received from eight Romanian counties. For comparison, 102 isolates from non-invasive disease, as well as a collection of 12 old invasive strains (isolated between 1967 and 1980) were included. All isolates were characterized by several methods: T and emm typing, presence of the fibronectin-binding protein F1 gene (prtF1), serum opacity factor (sof), and superantigen (SAg) genes (speA, speB, speC, speF, speG, speH, ssa and smeZ). The recent invasive isolates exhibited 19 emm-types, of which emm1, emm81, emm76, emm49 and emm78 covered 57 % of the strains. Furthermore, multilocus sequence typing analysis revealed nine new sequence types, corresponding to emm types 1, 12, 49, 81, 92, 100, 106 and 119. The non-invasive isolates comprised 24 different emm types with a predominance of emm1 and 12; the old invasive strains were of eight emm types, of which four were unique for this group. All isolates harboured speB and speF; smeZ was detected in all invasive strains, except for the emm49 and emm81 isolates. The majority of isolates from carriers, and patients with pharyngitis were prtF1 positive, most of these (14 strains) being emm12. High tetracycline resistance rates were noted among both invasive and control isolates (54 % and 35 %, respectively), whereas macrolide resistance rates were low (3 % and 5 %, respectively). Active and continuing surveillance is required to provide an accurate assessment of the disease burden and to provide epidemiological data on the character of isolates in Romania.


2008 ◽  
Vol 6 (4) ◽  
pp. 0-0
Author(s):  
Valentinas Uvarovas ◽  
Igoris Šatkauskas ◽  
Tomas Sveikata ◽  
Eduardas Bartkevičius

Valentinas Uvarovas, Igoris Šatkauskas, Tomas Sveikata, Eduardas BartkevičiusVilniaus greitosios pagalbos universitetinės ligoninės Ortopedijos ir traumatologijos centras,Šiltnamių g. 29, LT-04130 VilniusEl paštas: [email protected] Šio straipsnio tikslas – aparašyti nekrozinio fascito klinikinį atvejį, pateikti literatūros apžvalgą. Nekrozinis fascitas – gyvybei pavojinga labai greitai plintanti infekcija, kuri audiniuose gali plisti žaibišku greičiu. Ji sukelia didelį mirštamumą (miršta nuo 24% iki 40% ligonių) [1, 2] bei didelį traumatizmą. Nekrozinis fascitas apima paviršines fascijas, poodinius riebalus, nervus, venas, arterijas, giliąsias fascijas. Patofiziologiškai infekcija pasireiškia fibrinoidine arteriolių koaguliacija, kuri sukelia audinių išemiją ir nekrozę.Aprašomas klinikinis atvejis, kai 44 metų pacientei uždaras kulnakaulio išnirimas komplikavosi nekrotiziniu fascitu ir streptokokiniu sepsiniu šoku su dauginiu organų nepakankamumu. Klinika pasireiškė šeštą parą po traumos, pacientė kreipėsi pakartotinai į skubiosios traumatologinės pagalbos skyrių dėl stipraus čiurnos srities skausmo. Nekrozinis fascitas ir sepsinis šokas diagnozuotas po 36 val., kai buvo paguldyta į ligoninę: skausmas išplito iki šlaunies, atsirado pūslių blauzdoje ir šlaunyje. Pacientė operuota kelis kartus: atliktos fasciotomijos, nekrektomijos, žaizdų revizija, amputuota galūnė, vėliau dėl plaštakų ir kitos pėdos gangrenavimo atliktos likusių galūnių amputacijos. Pacientė dėl sepsinio šoko ir dauginio organų nepakankamumo gydyta intensyviosios terapijos skyriuje, hemodinamika buvo palaikoma didelėmis vazopresorių dozėmis, taikyta antibiotikų terapija, hemofiltracija. Pagrindinis ligos sukėlėjas – Streptococcus pyogenes. Ligonė išgyveno, bet liko neįgali. Prognozė priklauso nuo laiku atlikto ir radikalaus chirurginio gydymo. Ankstyva diagnostika ir standartizuotas gydymas pagerintų nekrozinio fascito gydymo rezultatus vertinant mirtingumą ir galūnių išsaugojimą. Reikšminiai žodžiai: nekrozinis fascitas, Streptococcus pyogenes, toksinis šokas Streptococcus pyogenes necrotizing fascitis after closed calcaneus luxation Valentinas Uvarovas, Igoris Šatkauskas, Tomas Sveikata, Eduardas BartkevičiusOrthopedic Traumatology Center of Vilnius University Emergency Hospital,Šiltnamių str. 29, LT-04130 Vilnius, LithuaniaE-mail: [email protected] The aim of this study was to present a rare case of necrotizing fascitis and review the literature. We report a case of a 44-year-old female patient with a right closed subtalar luxation whose treatment developed necrotizing fascitis, streptococcal toxic shock syndrome and ended up with amputation of four limbs. After closed reduction of the calcaneus and immobilization in plaster cast at our emergency department and 2 days of hospitalization, the patient was referred for outpatient care. Within 6 days, due to severe pain and moderate swelling of the right ankle joint and the calf, the patient was admitted to the emergency department for the second time. In 36 hours after hospitalization, the patient developed hypotension and a severe septic situation with progressive swelling of the right calf and thigh. After the diagnosis of necrotizing fascitis had been established, the patient underwent an urgent and aggressive surgical debridement with fasciotomies of the right lower extremity. The microbiological investigation of the intraoperatively taken specimens presented group A-ß-hemolytic streptococcus. The patient survived, but despite an antibiotic therapy, intensive care support and second book operation, the amputation of the right leg was done, and the treatment ended in the amputation of the left tibia and both arms. Key words: necrotizing fascitis, Streptococcus pyogenes, streptococcal toxic shock syndrome


IDCases ◽  
2017 ◽  
Vol 9 ◽  
pp. 85-88 ◽  
Author(s):  
Tetsuya Sakai ◽  
Daisuke Taniyama ◽  
Saeko Takahashi ◽  
Morio Nakamura ◽  
Takashi Takahashi

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