scholarly journals Comparison of MB/BacT ALERT 3D System with Radiometric BACTEC System and Lowenstein-Jensen Medium for Recovery and Identification of Mycobacteria from Clinical Specimens: a Multicenter Study

2001 ◽  
Vol 39 (2) ◽  
pp. 651-657 ◽  
Author(s):  
C. Piersimoni ◽  
C. Scarparo ◽  
A. Callegaro ◽  
C. P. Tosi ◽  
D. Nista ◽  
...  
Keyword(s):  
Multicenter Study ◽  
Clinical Specimens ◽  
Lowenstein Jensen ◽  
Bactec System

scholarly journals Multicenter Comparison of ESP Culture System II with BACTEC 460TB and with Lowenstein-Jensen Medium for Recovery of Mycobacteria from Different Clinical Specimens, Including Blood

1998 ◽  
Vol 36 (5) ◽  
pp. 1378-1381 ◽  
Author(s):  
Enrico Tortoli ◽  
Paola Cichero ◽  
M. Gabriella Chirillo ◽  
M. Rita Gismondo ◽  
Letizia Bono ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Avium ◽  
Culture System ◽  
Solid Medium ◽  
Mycobacterial Species ◽  
Becton Dickinson ◽  
Clinical Specimens ◽  
Lowenstein Jensen ◽  
Bactec System ◽  
The Times

The recently developed ESP Culture System II (AccuMed, Chicago, Ill.) was compared with radiometric BACTEC 460TB (Becton Dickinson, Towson, Md.) and with Lowenstein-Jensen medium for recovery of mycobacteria from over 2,500 clinical specimens both of respiratory and nonrespiratory origin, including blood. The majority of the 219 mycobacterial isolates (129) belonged to the Mycobacterium tuberculosis complex, followed by 37 isolates of theMycobacterium avium complex (MAC) and 53 isolates of eight other mycobacterial species. Rates of recovery obtained with BACTEC, ESP, and Lowenstein-Jensen medium were 89, 79, and 64%, respectively, with such differences being statistically significant. Different media and systems appeared to behave differently when the more frequently detected organisms were considered: M. tuberculosis complex isolates grew better with BACTEC, and MAC isolates grew better with ESP. An analysis of the combinations of Lowenstein-Jensen medium with BACTEC and with ESP did not reveal significant differences in recovery rates. With regard to the times needed for the detection of positive cultures, they were significantly longer on Lowenstein-Jensen medium (average, 28 days) than with the remaining two systems, between which there was no difference (average, 18 days). We conclude, therefore, that the ESP system, when used in combination with a solid medium, performs as well as the thoroughly validated radiometric BACTEC system and offers the advantages of full automation and absence of radioisotopes.

scholarly journals Comparison of recoveries of Mycobacterium tuberculosis using the automated BACTEC MGIT 960 System and Lowenstein – Jensen medium

2010 ◽  
Vol 9 (1) ◽  
pp. 44
Author(s):  
Mo. A.AL-Mazini, T. Bukeet, and A. Abdul Kareem
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Recovery Rates ◽  
Clinical Specimens ◽  
Indicator Tube ◽  
Lowenstein Jensen ◽  
Bactec System ◽  
Smear Negative ◽  
The Difference ◽  
Growth Indicator

We examined whether the BACTEC/ Mycobacteria Growth Indicator Tube (MGIT) System alone could supplant the use of a supplemental Lowenstein–Jensen (LJ) slant for routine recovery of M. tuberculosis from clinical specimens. A total of 392 specimens of sputum were included in the study, collected from 196 patients. Specimens were processed with standard N-acetyl- L- Cysteine (NALC-NaOH) method, then inoculated onto BACTEC MGIT 960 and onto LJ media. The recovery rates of M.tuberculosis were 100 % (256/256) with BACTEC MGIT 960 and 72.6%(186/256) with LJ. The rates of contamination for each of the system were 4.8%with BACTEC MGIT 960 and 5.3%with LJ. The TTD for M. tuberculosis was 11.3 days with BACTEC System and 30.8 days with LJ. The difference in TTD between smear positive and smear negative specimens for M.tuberculosis with BACTEC MGIT 960 was not statistically significant. This study shows that the BACTEC System demonstrates better sensitivity for the recovery of M. tuberculosis from clinical specimens.

Comparison of the VersaTREK system and Löwenstein-Jensen medium for the recovery of mycobacteria from clinical specimens

2008 ◽  
Vol 40 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Francisco Quiñones Falconi ◽  
Lourdes Infante Suárez ◽  
Manuel de jesús castillejos López ◽  
Cecilia garcía Sancho
Keyword(s):  
Clinical Specimens ◽  
Lowenstein Jensen

scholarly journals The influence of vitamin C on the urine dipstick tests in the clinical specimens: a multicenter study

2016 ◽  
Vol 31 (5) ◽  
pp. e22080 ◽  
Author(s):  
Wonmok Lee ◽  
Yukyung Kim ◽  
Soonhee Chang ◽  
A-jin Lee ◽  
Chang-Ho Jeon
Keyword(s):  
Vitamin C ◽  
Multicenter Study ◽  
Clinical Specimens ◽  
Urine Dipstick

scholarly journals Effects of Decontamination Methods and Culture Conditions on Viability of Mycobacterium ulcerans in the BACTEC System

1998 ◽  
Vol 36 (2) ◽  
pp. 402-408 ◽  
Author(s):  
J. C. Palomino ◽  
F. Portaels
Keyword(s):  
Geographic Origin ◽  
Egg Yolk ◽  
Polymyxin B ◽  
Culture Conditions ◽  
Mycobacterium Ulcerans ◽  
Clinical Specimens ◽  
Geographic Origins ◽  
Primary Cultivation ◽  
Bactec System ◽  
Detrimental Impact

We used the BACTEC system to evaluate the effects of several decontamination methods and the addition of antibiotics on the viability of Mycobacterium ulcerans. The effects of polyoxyethylene stearate or egg yolk as supplements were also evaluated to determine their impact on the growth of M. ulcerans. Strains of different geographic origins were subjected to Petroff, reversed Petroff, oxalic acid, and mild HCl treatments. After treatment, the viability of each strain was assessed in the BACTEC system. All of the decontamination methods tested adversely affected bacterial viability. Treatment with mild HCl gave the best results, allowing better growth rates with some strains and causing a delay in growth with others, depending on the geographic origin of the strain. A mixture of polymyxin B, amphotericin B, nalidixic acid, trimethoprim, and azlocillin did not significantly inhibit growth. Supplementing BACTEC medium with egg yolk markedly improved the recovery of M. ulcerans following the use of each of the decontamination methods. Our findings demonstrate a detrimental impact on the viability of M. ulcerans by all of the decontamination methods currently in common use. This explains, at least in part, the difficulty often experienced in cultivating this organism from clinical specimens. Egg yolk should be added to enhance the rate of successful primary cultivation of M. ulcerans in the BACTEC system.

scholarly journals A Novel Solid Medium for Culturing Mycobacterium tuberculosis Isolates from Clinical Specimens

2015 ◽  
Vol 53 (8) ◽  
pp. 2566-2569 ◽  
Author(s):  
S. Asmar ◽  
S. Chatellier ◽  
C. Mirande ◽  
A. van Belkum ◽  
I. Canard ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Solid Medium ◽  
Laboratory Diagnosis ◽  
Contamination Rate ◽  
Content Type ◽  
Exact Test ◽  
Clinical Specimens ◽  
Fisher's Exact Test ◽  
Fisher’S Exact Test ◽  
Lowenstein Jensen

The laboratory diagnosis of tuberculosis usually relies on culture-based isolation of the causativeMycobacterium tuberculosisbacteria. We developed and evaluated the performance of MOD9, a new blood-free derivative of the MOD4 solid medium on which we previously reported for the isolation and culture of mycobacteria. First, inoculation of Lowenstein-Jensen medium with 21M. tuberculosisisolates at 105, 103, and 10 CFU yielded colonies in 5.7 ± 1.5 days, 7.6 ± 0.8 days, and 10.8 ± 1.7 days versus 1.5 ± 0.4 days, 3.5 ± 0.6 days, and 4.9 ± 1 days in MOD9 (P< 0.05, Student'sttest). Further, the time to detectable growth ofM. tuberculosiswas measured on MOD9 and Lowenstein-Jensen media after duplicate inoculation of 250 clinical specimens decontaminated with 0.7% chlorhexidine. The contamination rate was 1.6% (4/250) on MOD9 versus 4.4% (11/250) on Lowenstein-Jensen medium (P= 0.11, Fisher's exact test). Chlorhexidine-MOD9 yielded 38/250 (15.2%) isolates versus 32/250 (12.8%) isolates for the chlorhexidine-LJ (P= 0.5195, Fisher's exact test). All together, eightM. tuberculosisisolates were cultured solely from chlorhexidine-MOD9, and twoM. tuberculosisisolates were cultured solely from chlorhexidine-LJ. The time to detection was 9.8 ± 3.9 (range, 5 to 18) days for chlorhexidine-MOD9 versus 17.4 ± 5.9 (range, 10 to 35) days for chlorhexidine-LJ (P< 0.05, Student'sttest). These data indicate the superiority of the MOD9 medium over the standard LJ medium following chlorhexidine decontamination for the recovery ofM. tuberculosis.

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