Semiautomation of Multilocus Sequence Typing for the Characterization of Clinical Isolates of Neisseria meningitidis

Three group BNeisseria meningitidisisolates, recovered from meningococcal disease cases in Canada and typed as B:2c:P1.5, were characterized. Multilocus sequence typing showed that all three isolates were related because of an identical sequence type (ST) 573. Isolates typed as 2c:P1.5 are common in serogroup Y meningococci but rare in isolates from serogroups B or C. Although no serogroup Y isolates have been typed as ST-573, eight isolates showed five to six housekeeping gene alleles that were identical to that of ST-573. This suggested that the B:2c:P1.5 isolates may have originated from serogroup Y organisms, possibly by capsule switching.

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Characterization of Neisseria meningitidis isolates collected from 1974 to 2003 in Japan by multilocus sequence typing

Journal of Medical Microbiology ◽

10.1099/0022-1317-53-11-1175 ◽

2004 ◽

Vol 53 (11) ◽

pp. 1175-1175

Author(s):

H Takahashi ◽

T Kuroki ◽

Y Watanabe ◽

H Tanaka ◽

H Inouye ◽

...

Keyword(s):

Neisseria Meningitidis ◽

Multilocus Sequence Typing

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Characterization of Neisseria meningitidis isolates from Egypt using multilocus sequence typing

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1016/j.trstmh.2012.01.010 ◽

2012 ◽

Vol 106 (5) ◽

pp. 309-314 ◽

Cited By ~ 4

Author(s):

John D. Klena ◽

Momtaz O. Wasfy ◽

Rania A. Nada ◽

Salwa F. Ahmed ◽

Mohamed A. Maksoud ◽

...

Keyword(s):

Neisseria Meningitidis ◽

Multilocus Sequence Typing

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Characterization of Neisseria meningitidis isolates collected from 1974 to 2003 in Japan by multilocus sequence typing

Journal of Medical Microbiology ◽

10.1099/jmm.0.45541-0 ◽

2004 ◽

Vol 53 (7) ◽

pp. 657-662 ◽

Cited By ~ 37

Author(s):

Hideyuki Takahashi ◽

Toshiro Kuroki ◽

Yuko Watanabe ◽

Hiroshi Tanaka ◽

Hiroo Inouye ◽

...

Keyword(s):

Statistical Analysis ◽

Neisseria Meningitidis ◽

Multilocus Sequence Typing ◽

Clonal Complex ◽

The Other ◽

Mlst Database ◽

Mlst Analysis ◽

The Past ◽

Sequence Types

Analysis of 182 Neisseria meningitidis strains isolated over the past 30 years in Japan by serogroup typing and multilocus sequence typing (MLST) was performed. The serogroups of the 182 Japanese isolates were B (103 isolates), Y (39), W135 (1) and non-groupable (39). By MLST analysis, 65 different sequence types (ST) were identified, 42 of which were not found in the MLST database as of January 2004 and seemed to be unique to Japan. Statistical analysis of the MLST results revealed that, although the Japanese isolates seemed to be genetically divergent, they were classified into six major clonal complexes and other minor complexes. Among these isolates, well-documented ST complexes found worldwide were present, such as ST-23 complex (49 isolates), ST-44 complex (41 isolates) and ST-32 complex (8 isolates). On the other hand, a new clonal complex designated ST-2046 complex (28 isolates), which has not been identified in other countries, was also found, suggesting that this clone was indigenous to Japan. Taken together, it was speculated that meningococcal isolates in Japan comprised heterogeneous clones, which were derived both from clones identified in other countries and clones unique to Japan.

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