Species-Specific Serodiagnosis of Lyme Arthritis and Neuroborreliosis Due to Borrelia burgdorferi Sensu Stricto, B. afzelii, and B. garinii by Using Decorin Binding Protein A

ABSTRACT Arthritis is a frequent and major complication of infection withBorrelia burgdorferi sensu stricto. The antigens responsible for the induction of arthritis are unknown. Here we provide direct evidence that a major surface protein, outer surface protein A (OspA), can induce arthritis. Hamsters were vaccinated with 30, 60, or 120 μg of recombinant OspA (rOspA) in aluminum hydroxide and challenged with B. burgdorferi sensu stricto isolate 297 or C-1-11. Swelling of the hind paws was detected in 100, 100, and 50% of hamsters vaccinated with 30, 60, or 120 μg of rOspA, respectively. In addition, arthritis developed in 57% of hamsters vaccinated with a canine rOspA vaccine after infection with B. burgdorferisensu stricto. When the canine rOspA vaccine was combined with aluminum hydroxide, all vaccinated hamsters developed arthritis after challenge with B. burgdorferi sensu stricto. Histopathologic examination confirmed the development of severe destructive arthritis in rOspA-vaccinated hamsters challenged with B. burgdorferisensu stricto. These findings suggest that rOspA vaccines should be modified to eliminate epitopes of OspA responsible for the induction of arthritis. Our results are important because an rOspA vaccine in aluminum hydroxide was approved by the Food and Drug Administration for use in humans.

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Is Fibromyalgia Associated with Borrelia Specific T Lymphocytes?

Current Rheumatology Reviews ◽

10.2174/1573397117666210924152636 ◽

2021 ◽

Vol 17 ◽

Author(s):

Basant K. Puri ◽

Gary S. Lee ◽

Armin Schwarzbach

Keyword(s):

T Lymphocytes ◽

Borrelia Burgdorferi ◽

T Lymphocyte ◽

Protein A ◽

Sensu Stricto ◽

Controlled Study ◽

Lymphocyte Function ◽

Group Status ◽

Borrelia Burgdorferi Sensu Stricto ◽

Lymphocyte Reactivity

Background: Although fibromyalgia is a common cause of chronic musculoskeletal pain, its aetiology and pathophysiology are uncertain. It has recently been suggested that fibromyalgia symptomatology represents a T lymphocyte-mediated immune response to pathogens which are known risk factors for autoimmune diseases. One major suggested candidate pathogen is the bacterial genus Borrelia. However, to date this hypothesis has not been tested. Objective: The aim was to carry out the first test of this hypothesis by comparing Borrelia-specific T lymphocyte reactivity in fibromyalgia patients and matched controls. Methods: The enzyme-linked immunospot was used to detect T-lymphocyte reactivity to Borrelia burgdorferi sensu stricto (full antigen); outer surface protein (Osp) A from Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii; native OspC plus decorin binding protein A recombinant; and lymphocyte function antigen-1 (shared epitope) in 27 patients who fulfilled the revised diagnostic criteria for fibromyalgia of the American College of Rheumatology and in 26 control subjects. The assays were carried out blind to group status. Results: The two groups did not differ by age, sex or ethnicity. They did not differ significantly in respect of T lymphocyte reactivity to Borrelia burgdorferi sensu stricto (full antigen) (p = 0.847), Osp mix (p = 0.709) or lymphocyte function antigen-1 (p = 0.367). Conclusion: This novel controlled study provides no evidence of an association between fibromyalgia and Borrelia-specific T lymphocytes.

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PREPARATION AND PURIFICATION OF RECOMBINANT OUTER SURFACE PROTEIN A (rOspA) OF BORRELIA BURGDORFERI SENSU STRICTO AND BORRELIA AFZELII

Biomedical Papers ◽

10.5507/bp.2005.035 ◽

2005 ◽

Vol 149 (2) ◽

pp. 257-259 ◽

Cited By ~ 2

Author(s):

Jana Belakova ◽

Michal Krupka ◽

Martina Sebestova ◽

Jana Tuhackova ◽

Vladimir Vrzal ◽

...

Keyword(s):

Borrelia Burgdorferi ◽

Outer Surface ◽

Protein A ◽

Surface Protein ◽

Sensu Stricto ◽

Borrelia Afzelii ◽

Outer Surface Protein A ◽

Outer Surface Protein ◽

Borrelia Burgdorferi Sensu Stricto

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Scored Antibody Reactivity Determined by Immunoblotting Shows an Association between Clinical Manifestations and Presence of Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, and B. Valaisianain Humans

Journal of Clinical Microbiology ◽

10.1128/jcm.37.12.4086-4092.1999 ◽

1999 ◽

Vol 37 (12) ◽

pp. 4086-4092 ◽

Cited By ~ 50

Author(s):

Karine Ryffel ◽

Olivier Péter ◽

Bernard Rutti ◽

André Suard ◽

Eric Dayer

Keyword(s):

Borrelia Burgdorferi ◽

Clinical Manifestations ◽

Sensu Stricto ◽

Lyme Arthritis ◽

Serum Samples ◽

Scoring Method ◽

Pathogenic Potential ◽

Acrodermatitis Chronica Atrophicans ◽

Additional Protein ◽

Borrelia Burgdorferi Sensu Stricto

An immunoglobulin G immunoblot was developed with antigenic extracts of Borrelia burgdorferi sensu stricto,B. garinii, B. afzelii, and B. valaisiana genospecies and was reacted with sera from patients with neuroborreliosis, acrodermatitis, and Lyme arthritis. A detailed analysis of the reactivities of the protein bands was performed, and a two-step scoring procedure was selected to determine the preferential reactivity of sera to one particular genospecies. The discriminative potential of 5 proteins (12-kDa, 16-kDa, 18-kDa, OspA, and 66-kDa proteins) was used as a rapid first-step scoring method, followed by scoring of 14 additional protein bands if necessary. The advantage of this procedure is the low percentage of serum samples with inconclusive results for one of the four species (10% for patients with neuroborreliosis, 6% for patients with acrodermatitis chronica atrophicans, and 6% for patients with Lyme arthritis). Among 31 serum samples from patients with neuroborreliosis, 16 were more reactive toB. garinii, 7 were more reactive to B. afzelii, 3 were more reactive to B. valaisiana, and 2 were more reactive to B. burgdorferi sensu stricto. Of 31 serum samples from patients with acrodermatitis, 26 showed a higher level of reactivity to B. afzelii. Of 34 serum samples from patients with Lyme arthritis, 21 were more reactive to B. burgdorferi sensu stricto, 10 were more reactive to B. afzelii, and 1 was more reactive to B. valaisiana. Our results suggest an organotropism of Borrelia species and provide some evidence of a pathogenic potential of B. valaisiana in humans.

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