scholarly journals Instability of the Restriction Fragment Length Polymorphism Pattern of Open Reading Frame 5 of Porcine Reproductive and Respiratory Syndrome Virus during Sequential Pig-to-Pig Passages

2004 ◽  
Vol 42 (10) ◽  
pp. 4462-4467 ◽  
Author(s):  
S.-H. Cha ◽  
C.-C. Chang ◽  
K.-J. Yoon
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Open Reading Frame ◽  
Respiratory Syndrome Virus ◽  
Reading Frame ◽  
Polymorphism Pattern ◽  
Restriction Fragment Length

scholarly journals Complete Coding Genome Sequence of a Novel Porcine Reproductive and Respiratory Syndrome Virus 2 Restriction Fragment Length Polymorphism 1-4-4 Lineage 1C Variant Identified in Iowa, USA

2021 ◽  
Vol 10 (21) ◽  
Author(s):  
Giovani Trevisan ◽  
Ganwu Li ◽  
Cesar A. A. Moura ◽  
Katie Coleman ◽  
Pete Thomas ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Genome Sequence ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Respiratory Syndrome Virus ◽  
Swine Farm ◽  
Reading Frame ◽  
Restriction Fragment Length

A porcine reproductive and respiratory syndrome virus 2 strain was identified in lung samples from nursery piglets associated with a 17.15% mortality rate on a swine farm in Iowa, USA. Open reading frame 5 (ORF5) sequencing indicated that this strain is a restriction fragment length polymorphism (RFLP) 1-4-4 lineage 1C variant strain, and its complete coding genome sequence was determined.

scholarly journals Differentiation of a Porcine Reproductive and Respiratory Syndrome Virus Vaccine Strain from North American Field Strains by Restriction Fragment Length Polymorphism Analysis of ORF 5

1998 ◽  
Vol 10 (2) ◽  
pp. 140-144 ◽  
Author(s):  
Ronald D. Wesley ◽  
William L. Mengeling ◽  
Kelly M. Lager ◽  
Deborah F. Clouser ◽  
John G. Landgraf ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Vaccine Strain ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Parent Strain ◽  
Fragment Length Polymorphism ◽  
Vaccine Virus ◽  
Respiratory Syndrome Virus ◽  
Restriction Fragment Length

The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.

scholarly journals Differentiation of porcine reproductive and respiratory syndrome virus European vaccine strains from Czech field isolates by restriction fragment length polymorphism analysis of ORF5 gene

2012 ◽  
Vol 47 (No. 10 - 11) ◽  
pp. 295-301 ◽  
Author(s):  
S. Indik ◽  
L. Valíček
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Respiratory Syndrome Virus ◽  
Field Isolates ◽  
Restriction Fragment Length

Restriction fragment length polymorphism (RFLP) analysis of open reading frame 5 was developed for typing of Czech strains of porcine reproductive and respiratory syndrome virus (PRRSV). The set of restriction enzymes Acc I, Hae II and SnaB I allowed the differentiation of heterogeneous Czech strains of PRRSV clustered separately in the phylogenetic tree. The high-passage strain V-502 (164) was also differentiated from its parent strain V-502. The same restriction enzymes could distinguish the European-type vaccine strains Porcilis PRRS and Pyrsvac-183, registered inCzechRepublic, from the Czech field isolates. The published ORF5 nucleotide sequences allowed us to presume that it will also be possible to distinguish most of European field strains from vaccine strains. PCR-based RFLP analysis can become a valuable tool in epidemiological studies of PRRSV inEurope.

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