Differentiation of a Porcine Reproductive and Respiratory Syndrome Virus Vaccine Strain from North American Field Strains by Restriction Fragment Length Polymorphism Analysis of ORF 5
1998 ◽
Vol 10
(2)
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pp. 140-144
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Keyword(s):
The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.
2004 ◽
Vol 42
(10)
◽
pp. 4462-4467
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2000 ◽
Vol 76
(3)
◽
pp. 221-228
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2012 ◽
Vol 47
(No. 10 - 11)
◽
pp. 295-301
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2001 ◽
Vol 63
(11)
◽
pp. 1203-1207
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