Ten Years of Global Evolution of the Human Respiratory Syncytial Virus BA Genotype with a 60-Nucleotide Duplication in the G Protein Gene

ABSTRACT The emergence of natural isolates of human respiratory syncytial virus group B (HRSV-B) with a 60-nucleotide (nt) duplication in the G protein gene in Buenos Aires, Argentina, in 1999 (A. Trento et al., J. Gen. Virol. 84:3115-3120, 2003) and their dissemination worldwide allowed us to use the duplicated segment as a natural tag to examine in detail the evolution of HRSV during propagation in its natural host. Viruses with the duplicated segment were all clustered in a new genotype, named BA (A. Trento et al., J. Virol. 80:975-984, 2006). To obtain information about the prevalence of these viruses in Spain, we tested for the presence of the duplicated segment in positive HRSV-B clinical samples collected at the Severo Ochoa Hospital (Madrid) during 12 consecutive epidemics (1996-1997 to 2007-2008). Viruses with the 60-nt duplication were found in 61 samples, with a high prevalence relative to the rest of B genotypes in the most recent seasons. Global phylogenetic and demographic analysis of all G sequences containing the duplication, collected across five continents up until April 2009, revealed that the prevalence of the BA genotype increased gradually until 2004-2005, despite its rapid dissemination worldwide. After that date and coinciding with a bottleneck effect on the population size, a relatively new BA lineage (BA-IV) replaced all other group B viruses, suggesting further adaptation of the BA genotype to its natural host.

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Glycosylation studies of G-protein of BA genotype of group B human respiratory syncytial virus in mammalian cells

10.1183/13993003.congress-2015.pa2673 ◽

2015 ◽

Author(s):

Wajihul Khan ◽

Raghuram VLN Shrungaram ◽

Shobha Broor ◽

Shama Parveen

Keyword(s):

Respiratory Syncytial Virus ◽

G Protein ◽

Mammalian Cells ◽

Human Respiratory Syncytial Virus ◽

Syncytial Virus ◽

Group B ◽

Ba Genotype

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Natural History of Human Respiratory Syncytial Virus Inferred from Phylogenetic Analysis of the Attachment (G) Glycoprotein with a 60-Nucleotide Duplication

Journal of Virology ◽

10.1128/jvi.80.2.975-984.2006 ◽

2006 ◽

Vol 80 (2) ◽

pp. 975-984 ◽

Cited By ~ 112

Author(s):

Alfonsina Trento ◽

Mariana Viegas ◽

Mónica Galiano ◽

Cristina Videla ◽

Guadalupe Carballal ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Respiratory Syncytial Virus ◽

Natural History ◽

G Protein ◽

Buenos Aires ◽

Protein Gene ◽

Human Respiratory Syncytial Virus ◽

Clinical Samples ◽

History Of ◽

Syncytial Virus

ABSTRACT A total of 47 clinical samples were identified during an active surveillance program of respiratory infections in Buenos Aires (BA) (1999 to 2004) that contained sequences of human respiratory syncytial virus (HRSV) with a 60-nucleotide duplication in the attachment (G) protein gene. This duplication was analogous to that previously described for other three viruses also isolated in Buenos Aires in 1999 (A. Trento et al., J. Gen. Virol. 84:3115-3120, 2003). Phylogenetic analysis indicated that BA sequences with that duplication shared a common ancestor (dated about 1998) with other HRSV G sequences reported worldwide after 1999. The duplicated nucleotide sequence was an exact copy of the preceding 60 nucleotides in early viruses, but both copies of the duplicated segment accumulated nucleotide substitutions in more recent viruses at a rate apparently higher than in other regions of the G protein gene. The evolution of the viruses with the duplicated G segment apparently followed the overall evolutionary pattern previously described for HRSV, and this genotype has replaced other prevailing antigenic group B genotypes in Buenos Aires and other places. Thus, the duplicated segment represents a natural tag that can be used to track the dissemination and evolution of HRSV in an unprecedented setting. We have taken advantage of this situation to reexamine the molecular epidemiology of HRSV and to explore the natural history of this important human pathogen.

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Positive Selection Results in Frequent Reversible Amino Acid Replacements in the G Protein Gene of Human Respiratory Syncytial Virus

PLoS Pathogens ◽

10.1371/journal.ppat.1000254 ◽

2009 ◽

Vol 5 (1) ◽

pp. e1000254 ◽

Cited By ~ 90

Author(s):

Viviane F. Botosso ◽

Paolo M. de A. Zanotto ◽

Mirthes Ueda ◽

Eurico Arruda ◽

Alfredo E. Gilio ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Amino Acid ◽

Positive Selection ◽

G Protein ◽

Protein Gene ◽

Human Respiratory Syncytial Virus ◽

Syncytial Virus

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Molecular diversity of the aminoterminal region of the G protein gene of human respiratory syncytial virus subgroup B

Pathologie Biologie ◽

10.1016/j.patbio.2007.06.001 ◽

2008 ◽

Vol 56 (2) ◽

pp. 50-57 ◽

Cited By ~ 2

Author(s):

I. Fodha ◽

A. Vabret ◽

L. Bouslama ◽

M. Leroux ◽

L. Legrand ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

G Protein ◽

Molecular Diversity ◽

Protein Gene ◽

Human Respiratory Syncytial Virus ◽

Syncytial Virus

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Intrapatient Variation of the Respiratory Syncytial Virus Attachment Protein Gene

Journal of Virology ◽

10.1128/jvi.01181-10 ◽

2010 ◽

Vol 84 (19) ◽

pp. 10425-10428 ◽

Cited By ~ 13

Author(s):

Charles N. Agoti ◽

Jean L. Mbisa ◽

Ann Bett ◽

Graham F. Medley ◽

D. James Nokes ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

G Protein ◽

Protein Gene ◽

Point Mutations ◽

Virus Attachment ◽

Single Genome ◽

B Virus ◽

Intrapatient Variability ◽

Syncytial Virus ◽

Group B

ABSTRACT Intrapatient variability of the attachment (G) protein gene of respiratory syncytial virus (RSV) was examined using both population and single-genome sequencing. Samples from three patients infected with a group B virus variant which has a 60-nucleotide duplication in the G protein gene were examined. These samples were chosen because occasional mixed sequence bases were observed. In a minority of RSV genomes from these patients considerable variability was found, including point mutations, insertions, and deletions. Of particular note, the deletion of the exact portion of the gene which had been duplicated in some isolates was observed in viral RNAs from two patients.

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