scholarly journals Natural History of Human Respiratory Syncytial Virus Inferred from Phylogenetic Analysis of the Attachment (G) Glycoprotein with a 60-Nucleotide Duplication

2006 ◽  
Vol 80 (2) ◽  
pp. 975-984 ◽  
Author(s):  
Alfonsina Trento ◽  
Mariana Viegas ◽  
Mónica Galiano ◽  
Cristina Videla ◽  
Guadalupe Carballal ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Respiratory Syncytial Virus ◽  
Natural History ◽  
G Protein ◽  
Buenos Aires ◽  
Protein Gene ◽  
Human Respiratory Syncytial Virus ◽  
Clinical Samples ◽  
History Of ◽  
Syncytial Virus

ABSTRACT A total of 47 clinical samples were identified during an active surveillance program of respiratory infections in Buenos Aires (BA) (1999 to 2004) that contained sequences of human respiratory syncytial virus (HRSV) with a 60-nucleotide duplication in the attachment (G) protein gene. This duplication was analogous to that previously described for other three viruses also isolated in Buenos Aires in 1999 (A. Trento et al., J. Gen. Virol. 84:3115-3120, 2003). Phylogenetic analysis indicated that BA sequences with that duplication shared a common ancestor (dated about 1998) with other HRSV G sequences reported worldwide after 1999. The duplicated nucleotide sequence was an exact copy of the preceding 60 nucleotides in early viruses, but both copies of the duplicated segment accumulated nucleotide substitutions in more recent viruses at a rate apparently higher than in other regions of the G protein gene. The evolution of the viruses with the duplicated G segment apparently followed the overall evolutionary pattern previously described for HRSV, and this genotype has replaced other prevailing antigenic group B genotypes in Buenos Aires and other places. Thus, the duplicated segment represents a natural tag that can be used to track the dissemination and evolution of HRSV in an unprecedented setting. We have taken advantage of this situation to reexamine the molecular epidemiology of HRSV and to explore the natural history of this important human pathogen.

scholarly journals Ten Years of Global Evolution of the Human Respiratory Syncytial Virus BA Genotype with a 60-Nucleotide Duplication in the G Protein Gene

2010 ◽  
Vol 84 (15) ◽  
pp. 7500-7512 ◽  
Author(s):  
Alfonsina Trento ◽  
Inmaculada Casas ◽  
Ana Calderón ◽  
Maria L. Garcia-Garcia ◽  
Cristina Calvo ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
G Protein ◽  
Protein Gene ◽  
Natural Host ◽  
Human Respiratory Syncytial Virus ◽  
Clinical Samples ◽  
New Genotype ◽  
Syncytial Virus ◽  
Group B ◽  
Ba Genotype

ABSTRACT The emergence of natural isolates of human respiratory syncytial virus group B (HRSV-B) with a 60-nucleotide (nt) duplication in the G protein gene in Buenos Aires, Argentina, in 1999 (A. Trento et al., J. Gen. Virol. 84:3115-3120, 2003) and their dissemination worldwide allowed us to use the duplicated segment as a natural tag to examine in detail the evolution of HRSV during propagation in its natural host. Viruses with the duplicated segment were all clustered in a new genotype, named BA (A. Trento et al., J. Virol. 80:975-984, 2006). To obtain information about the prevalence of these viruses in Spain, we tested for the presence of the duplicated segment in positive HRSV-B clinical samples collected at the Severo Ochoa Hospital (Madrid) during 12 consecutive epidemics (1996-1997 to 2007-2008). Viruses with the 60-nt duplication were found in 61 samples, with a high prevalence relative to the rest of B genotypes in the most recent seasons. Global phylogenetic and demographic analysis of all G sequences containing the duplication, collected across five continents up until April 2009, revealed that the prevalence of the BA genotype increased gradually until 2004-2005, despite its rapid dissemination worldwide. After that date and coinciding with a bottleneck effect on the population size, a relatively new BA lineage (BA-IV) replaced all other group B viruses, suggesting further adaptation of the BA genotype to its natural host.

scholarly journals Positive Selection Results in Frequent Reversible Amino Acid Replacements in the G Protein Gene of Human Respiratory Syncytial Virus

2009 ◽  
Vol 5 (1) ◽  
pp. e1000254 ◽  
Author(s):  
Viviane F. Botosso ◽  
Paolo M. de A. Zanotto ◽  
Mirthes Ueda ◽  
Eurico Arruda ◽  
Alfredo E. Gilio ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Amino Acid ◽  
Positive Selection ◽  
G Protein ◽  
Protein Gene ◽  
Human Respiratory Syncytial Virus ◽  
Syncytial Virus

Genetic variability in the G protein gene of human respiratory syncytial virus isolated from the Campinas metropolitan region, Brazil

2008 ◽  
Vol 80 (9) ◽  
pp. 1653-1660 ◽  
Author(s):  
Luciana Helena Antoniassi da Silva ◽  
Fernando Rosado Spilki ◽  
Adriana Gut Lopes Riccetto ◽  
Renata Servan de Almeida ◽  
Emílio Carlos Elias Baracat ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Genetic Variability ◽  
G Protein ◽  
Protein Gene ◽  
Human Respiratory Syncytial Virus ◽  
Metropolitan Region ◽  
Syncytial Virus

scholarly journals Molecular epidemiology of human respiratory syncytial virus with severe acute respiratory infection in Huzhou from 2016 to 2019

2021 ◽  
Author(s):  
Deshun Xu ◽  
Lei Ji ◽  
Xiaofang Wu ◽  
Wei Yan ◽  
Liping Chen
Keyword(s):  
Phylogenetic Analysis ◽  
Respiratory Syncytial Virus ◽  
Nucleic Acid ◽  
G Protein ◽  
Respiratory Infection ◽  
Detection Rate ◽  
Human Respiratory Syncytial Virus ◽  
Age Group ◽  
Severe Acute Respiratory Infection ◽  
Syncytial Virus

AbstractBackgroundHuman respiratory syncytial virus (HRSV) is one of the major cause of acute lower respiratory infection in infants, the elderly and people with low immunity worldwide. Based on antigenic and genetic variations, Human respiratory syncytial virus is divided into two subgroups (A and B). Each of the subgroups is further categorized into genotypes based on the phylogenetic analyses of the sequences of the second hypervariable region.MethodsNasopharyngeal swabs (NPSs) were collected from patients of the First People’s Hospital in Huzhou from January 2016 to December 2019. Real-time RT-PCR (qPCR) was performed using double nucleic acid detection kit for respiratory syncytial virus (A\B) (Shenzhen shengkeyuan) with the ABI Q7 (Applied Biosystems). For genotyping, the primer set A-F/A-R was used to amplify the G protein of HRSV-A. Primer set B-F/B-R was used to amplify the G protein of HRSV-B. The phylogenetic analysis was constructed using the neighbor-joining algorithm with the Kimura two-parameter model and supported statistically by bootstrapping with 1000 replicates with MEGA software (version 7.0) with 1000 bootstrap replicates.ResultsA total of 973 nasopharyngeal swab samples were collected from January 2016 to December 2019, and 63 samples were positive for RSV nucleic acid, with the detection rate of 6.47%. Of the positive specimens, 28 were belonged to HRSV-A, and 35 were belonged to HRSV-B. Infection with RSV was found in all age groups tested, with the 0-1 year age group having the highest detection rate 15.2%. The detection rate was high from November to next March. Phylogenetic analysis clustered HRSV-A strains identified in Huzhou into ON1genotype. All 17 of the HRSV-B strains belonged to BA9 genotype.ConclusionsWe analyzed the HRSV strains circulation in Huzhou from January 2016 to December 2019 in Huzhou, China. This is the first molecular analysis on HRSV in Huzhou. We found Subgroup A and B of RSV were co-circulating and the 0-1 year age group having the highest infection rate.

scholarly journals Human Respiratory Syncytial Virus-Induced Immune Signature of Infection Revealed by Transcriptome Analysis of Clinical Pediatric Nasopharyngeal Swab Samples

2020 ◽  
Author(s):  
Claire Nicolas De Lamballerie ◽  
Andrés Pizzorno ◽  
Julia Dubois ◽  
Blandine Padey ◽  
Thomas Julien ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Transcriptome Analysis ◽  
Real Life ◽  
Human Respiratory Syncytial Virus ◽  
Clinical Samples ◽  
Nasopharyngeal Swab ◽  
Immune Signature ◽  
Real Life Setting ◽  
Syncytial Virus ◽  
New Diagnosis

Abstract Human respiratory syncytial virus (HRSV) constitutes one the main causes of respiratory infection in neonates and infants worldwide. Transcriptome analysis of clinical samples using high-throughput technologies remains an important tool to better understand virus-host complex interactions in the real-life setting but also to identify new diagnosis/prognosis markers or therapeutics targets. A major challenge when exploiting clinical samples such as nasal swabs, washes, or bronchoalveolar lavages is the poor quantity and integrity of nucleic acids. In this study, we applied a tailored transcriptomics workflow to exploit nasal wash samples from children who tested positive for HRSV. Our analysis revealed a characteristic immune signature as a direct reflection of HRSV pathogenesis and highlighted putative biomarkers of interest such as IP-10, TMEM190, MCEMP1, and TIMM23.

scholarly journals Biophysical and flavonoid-binding studies of the G protein ectodomain of group A human respiratory syncytial virus

Heliyon ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. e01394
Author(s):  
Vitor Brassolatti Machado ◽  
Jéssica Maróstica de Sá ◽  
Ana Karla Miranda Prado ◽  
Karina Alves de Toledo ◽  
Luis Octávio Regasini ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
G Protein ◽  
Human Respiratory Syncytial Virus ◽  
Binding Studies ◽  
Group A ◽  
Syncytial Virus

scholarly journals Genotyping of Type A Human Respiratory Syncytial Virus Based on Direct F Gene Sequencing

Medicina ◽  
2019 ◽  
Vol 55 (5) ◽  
pp. 169 ◽  
Author(s):  
Daifullah Al Aboud ◽  
Nora M. Al Aboud ◽  
Mater I. R. Al-Malky ◽  
Ahmed S. Abdel-Moneim
Keyword(s):  
Respiratory Syncytial Virus ◽  
Mutational Analysis ◽  
Gene Sequencing ◽  
Human Respiratory Syncytial Virus ◽  
Clinical Samples ◽  
Escape Mutant ◽  
Respiratory Pathogens ◽  
Virus Genotype ◽  
F Gene ◽  
Syncytial Virus

Background and objectives: The human respiratory syncytial virus (hRSV) is among the important respiratory pathogens affecting children. Genotype-specific attachment (G) gene sequencing is usually used to determine the virus genotype. The reliability of the fusion (F) gene vs. G gene genotype-specific sequencing was screened. Materials and Methods: Archival RNA from Saudi children who tested positive for hRSV-A were used. Samples were subjected to a conventional one-step RT-PCR for both F and G genes and direct gene sequencing of the amplicons using the same primer sets. Phylogeny and mutational analysis of the obtained sequences were conducted. Results: The generic primer set succeeded to amplify target gene sequences. The phylogenetic tree based on partial F gene sequencing resulted in an efficient genotyping of hRSV-A strains equivalent to the partial G gene genotyping method. NA1, ON1, and GA5 genotypes were detected in the clinical samples. The latter was detected for the first time in Saudi Arabia. Different mutations in both conserved and escape-mutant domains were detected in both F and G. Conclusion: It was concluded that a partial F gene sequence can be used efficiently for hRSV-A genotyping.

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