Chemical modification of simian virus 40 DNA by reaction with a water-soluble carbodiimide.

1976 ◽  
Vol 18 (1) ◽  
pp. 205-210 ◽  
Author(s):  
J Lebowitz ◽  
C G Garon ◽  
M C Chen ◽  
N P Salzman
Keyword(s):  
Chemical Modification ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Water Soluble

In Vitro Testing of Cyto- and Genotoxicity of New Porphyrin Water-Soluble Metal Derivatives

2007 ◽  
Vol 26 (6) ◽  
pp. 497-502 ◽  
Author(s):  
Gennadi Gasparyan ◽  
Galina Hovhannisyan ◽  
Robert Ghazaryan ◽  
Lida Sahakyan ◽  
Artak Tovmasyan ◽  
...  
Keyword(s):  
Clinical Investigation ◽  
Biological Effects ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Water Soluble ◽  
Trypan Blue Exclusion ◽  
African Green Monkey Kidney ◽  
Metal Derivatives

Porphyrins and porphyrin derivatives have an outstanding potential for discovery of novel pharmacological agents due to their ability for numerous chemical modifications and a variety of mechanisms of biological effects. New water-soluble Ag and Zn derivatives of tetrachloride meso-tetra (4- N-oxiethylpyridyl) porphyne were synthesized. Cyto- and genotoxicity of these substances were tested in vitro by the vital dye (trypan blue) exclusion and the micronucleus tests, respectively. Both metalloporphyrins were shown to be cytotoxic for Cos-7 (fibroblast-like African green monkey kidney cells transformed by simian virus 40 [SV40]), DU 145 (epithelial-like cells of human prostate carcinoma), and K-562 (human chronic myeloid leukemia cells) cell lines. At the same time they did not cause chromosome fragmentation in K-562 cell line at as high concentrations as IC50 (20 μmol/L for Ag and 70 μmol/L for Zn derivative). Thus, the metalloporphyrins tested meet at least two important demands to potential anticancer drugs as they combine the cytotoxicity with low genotoxicity. The three in vitro tumor models used are relevant to further in vitro and in vivo pre-clinical investigation of the studied metalloporphyrins as potential chemotherapeutics.

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