scholarly journals Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3

1998 ◽  
Vol 72 (2) ◽  
pp. 1394-1402 ◽  
Author(s):  
P. Seshidhar Reddy ◽  
Neeraja Idamakanti ◽  
Alexandre N. Zakhartchouk ◽  
Mohit Kumar Baxi ◽  
Joong Bok Lee ◽  
...  

ABSTRACT The complete DNA sequence of bovine adenovirus type 3 is reported here. The size of the genome is 34,446 bp in length with a G+C content of 54%. All the genes of the early and late regions are present in the expected locations of the genome. However, the late-region genes are organized into seven families, instead of five as they are in human adenovirus type 2. The deduced amino acid sequences of open reading frames (ORFs) in the late regions and early region 2 (E2) and for IVa2 show higher degrees of homology, whereas the predicted amino acid sequences of ORFs in the E1, E3, and E4 regions and the pIX, fiber, and 33,000-molecular-weight nonstructural proteins show little or no homology with the corresponding proteins of other adenoviruses. In addition, the penton base protein lacks the integrin binding motif, RGD, but has an LDV motif instead of an MDV motif. Interestingly, as in other animal adenoviruses, the virus-associated RNA genes appear to be absent from their usual location. Sequence analysis of cDNA clones representing the early- and late-region genes identified splice acceptor and splice donor sites, polyadenylation signals and polyadenylation sites, and tripartite leader sequences.

2006 ◽  
Vol 87 (12) ◽  
pp. 3539-3544 ◽  
Author(s):  
Li Xing ◽  
Suresh Kumar Tikoo

Conserved motifs of eukaryotic gene promoters, such as TATA box and CAAT box sequences, of E1A of human adenoviruses (e.g human adenovirus 5) lie between the left inverted terminal repeat (ITR) and the ATG of E1A. However, analysis of the left end of the bovine adenovirus 3 (BAdV-3) genome revealed that the conserved sequences of the E1A promoter are present only in the ITR. As such, the promoter activity of ITR was tested in the context of a BAdV-3 vector or a plasmid-based system. Different regions of the left end of the BAdV-3 genome initiated transcription of the red fluorescent protein gene in a plasmid-based system. Moreover, BAdV-3 mutants in which the open reading frame of E1A was placed immediately downstream of the ITR produced E1A transcript and could be propagated in non-E1A-complementing Madin–Darby bovine kidney cells. These results suggest that the left ITR contains the sole BAdV-3 E1A promoter.


1990 ◽  
Vol 18 (18) ◽  
pp. 5568-5568 ◽  
Author(s):  
Faxing Cai ◽  
Martin Bourbonnière ◽  
Denis Tang ◽  
Shiu-Lok Hu ◽  
Joseph M. Weber

Virology ◽  
2001 ◽  
Vol 290 (1) ◽  
pp. 153-163 ◽  
Author(s):  
Mohit K. Baxi ◽  
Jill Robertson ◽  
Lorne A. Babiuk ◽  
Suresh K. Tikoo

1995 ◽  
Vol 76 (1) ◽  
pp. 93-102 ◽  
Author(s):  
S. K. Mittal ◽  
L. Prevec ◽  
F. L. Graham ◽  
L. A. Babiuk

Nature ◽  
1966 ◽  
Vol 211 (5044) ◽  
pp. 102-102 ◽  
Author(s):  
J. H. DARBYSHIRE

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