Characterization of Unusual Escape Variants of Hepatitis B Virus Isolated from a Hepatitis B Surface Antigen-Negative Subject

ABSTRACT Hepatitis B virus DNA was extracted from serial serum samples of a hepatitis B surface antigen-negative patient with antibodies to the core protein as the only marker of an infection with hepatitis B virus. This patient showed no symptoms of hepatic injury. Sequencing of the amplified viral DNA demonstrated multiple amino acid changes clustering in surface-exposed regions of the surface protein. Synthesis and association of the middle (M) and small (S) surface proteins could be shown in vitro. The variant surface antigens were recognized neither by monoclonal antibodies to the surface antigen nor by the vaccinee’s sera. Consequences for hepatitis B surface antigen testing and vaccine development are discussed.

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Specific expression of human interferon-gamma controls hepatitis B virus replication in vitro in secreting hepatitis B surface antigen hepatocytes

Journal of Virological Methods ◽

10.1016/j.jviromet.2011.12.016 ◽

2012 ◽

Vol 180 (1-2) ◽

pp. 84-90 ◽

Cited By ~ 3

Author(s):

Quancheng Kan ◽

Duolu Li ◽

Zujiang Yu

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Interferon Gamma ◽

Virus Replication ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Human Interferon ◽

Specific Expression ◽

B Virus

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In vitro immune responses to hepatitis B surface antigen (pre-S2 and S) following remote infection by hepatitis B virus in humans

Journal of Clinical Immunology ◽

10.1007/bf00916819 ◽

1989 ◽

Vol 9 (3) ◽

pp. 229-241 ◽

Cited By ~ 3

Author(s):

Thomas R. Cupps ◽

Jay H. Hoofnagle ◽

Ronald W. Ellis ◽

William J. Miller ◽

Leonard Seeff ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Immune Responses ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Remote Infection ◽

B Virus

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Anti-idiotype modulation of the in vitro immune response to hepatitis B surface antigen (HBsAg) following remote infection by hepatitis B virus in man

Cellular Immunology ◽

10.1016/0008-8749(89)90164-0 ◽

1989 ◽

Vol 122 (1) ◽

pp. 244-250 ◽

Cited By ~ 3

Author(s):

Thomas R. Cupps ◽

Sally A. Haas-Smith ◽

John L. Gerin ◽

janyce L. Tibbles ◽

Ronald C. Kennedy

Keyword(s):

Immune Response ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Remote Infection ◽

B Virus ◽

In Vitro Immune Response

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Comparison of Three Luminescent Immunoassays for Hepatitis B Virus Surface Antigen Quantification during the Natural History of Chronic Hepatitis B Virus Infection

Clinical and Vaccine Immunology ◽

10.1128/cvi.00529-14 ◽

2014 ◽

Vol 21 (11) ◽

pp. 1521-1527 ◽

Cited By ~ 2

Author(s):

Xiao-Dong Cheng ◽

Liu-Wei Song ◽

Lin-Lin Fang ◽

Lin Yang ◽

Yong Wu ◽

...

Keyword(s):

Chronic Hepatitis ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Chronic Hepatitis B ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Serum Samples ◽

Virus Surface ◽

Chronic Hepatitis B Virus ◽

B Virus

ABSTRACTHepatitis B surface antigen (HBsAg) quantification has garnered attention because of its high predictive value in determining treatment responses. The HBsAg quantification assays, such as Architect and Elecsys, are commercially available, and more assays are in development. We aimed to compare the results of the Architect and Elecsys assays with those of a new assay, WTultra. The WTultra HBsAg assay is a sandwich chemiluminescent microplate enzyme immunoassay and provides an alternative choice which is more cost-effective and potentially applicable in developing or resource-constrained countries and areas. A total of 411 serum samples were collected from patients during various phases of chronic hepatitis B (CHB) infection. The samples were assessed using the three assays, and the results were compared and analyzed. The results for the Architect, Elecsys, and WTultra assays were well correlated according to the overall results for the samples (correlation coefficients,rArchitect versus WTultra= 0.936,rArchitect versus Elecsys= 0.952, andrWTultra versus Elecsys= 0.981) and the various infection phases (rArchitect versus WTultraranging from 0.67 to 0.975,rArchitect versus Elecsysranging from 0.695 to 0.982, andrWTultra versus Elecsysranging from 0.877 to 0.99). Additionally, consistent results were observed according to genotype (genotype B:rArchitect versus WTultra= 0.976,rArchitect versus Elecsys= 0.978, andrWTultra versus Elecsys= 0.979; genotype C:rArchitect versus WTultra= 0.950,rArchitect versus Elecsys= 0.963, andrWTultra versus Elecsys= 0.981) and hepatitis B virus (HBV) DNA levels (rArchitect= 0.540,rWTultra= 0.553, andrElecsys= 0.580). In conclusion, the Elecsys and WTultra assays were well correlated with the Architect assay, irrespective of the CHB infection phase or genotype. All of these assays are reliable for HBsAg quantification.

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