Mutation Spectra of Herpes Simplex Virus Type 1 Thymidine Kinase Mutants
2002 ◽
Vol 76
(11)
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pp. 5822-5828
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ABSTRACT To examine whether the exonuclease activity intrinsic to the polymerase (Pol) of herpes simplex virus type 1 can influence the mutational spectra, we applied the denaturing gradient gel electrophoresis (DGGE) system combined with sequencing to characterize thymidine kinase mutants isolated from both the wild-type virus and a mutant deficient in exonuclease activity, Y7. Wild-type viruses produced predominately frameshift mutations (67%), whereas Y7 replicated a significantly lower proportion of frameshifts (21%; P < 0.005). Furthermore, the majority of substitutions were transitional changes in both groups, although they distributed differently. The implications of these findings are discussed.
2003 ◽
Vol 77
(5)
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pp. 2946-2955
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2005 ◽
Vol 79
(19)
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pp. 12286-12295
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1993 ◽
Vol 67
(3)
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pp. 1482-1492
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Keyword(s):
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2019 ◽
Vol 38
(7)
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pp. 2116-2127
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1996 ◽
Vol 93
(8)
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pp. 3525-3529
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1999 ◽
Vol 264
(2)
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pp. 338-342
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1989 ◽
Vol 38
(12)
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pp. 1955-1961
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1996 ◽
Vol 77
(7)
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pp. 1521-1527
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2008 ◽
Vol 77
(1)
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pp. 77-80
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