Herpes Simplex Virus Type 1 Single-Strand DNA Binding Protein ICP8 Enhances the Nuclease Activity of the UL12 Alkaline Nuclease by Increasing Its Processivity
2005 ◽
Vol 79
(14)
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pp. 9356-9358
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ABSTRACT UL12 is a 5′- to 3′-exonuclease encoded by herpes simplex virus type 1 (HSV-1) which degrades single- and double-stranded DNA. UL12 and the single-strand DNA binding protein ICP8 mediate a strand exchange reaction. We found that ICP8 inhibited UL12 digestion of single-stranded DNA but stimulated digestion of double-stranded DNA threefold. The stimulatory effect of ICP8 was independent of a strand exchange reaction; furthermore, the effect was specific to ICP8, as it could not be reproduced by Escherichia coli single-stranded DNA binding protein. The effect of ICP8 on the rate of UL12 double-stranded DNA digestion is attributable to an increase in processivity in the presence of ICP8.
2016 ◽
Vol 35
(4)
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pp. 704-723
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1997 ◽
Vol 94
(7)
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pp. 2838-2842
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1988 ◽
Vol 69
(1)
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pp. 99-111
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1989 ◽
Vol 70
(9)
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pp. 2357-2364
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Keyword(s):
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1988 ◽
Vol 62
(3)
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pp. 818-825
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1986 ◽
Vol 83
(17)
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pp. 6322-6326
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1997 ◽
Vol 272
(36)
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pp. 22766-22770
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1991 ◽
Vol 266
(18)
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pp. 11413-11416
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