Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene
1991 ◽
Vol 11
(6)
◽
pp. 3374-3378
◽
Keyword(s):
The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.
1991 ◽
Vol 11
(6)
◽
pp. 3374-3378
◽
1995 ◽
Vol 91
(8)
◽
pp. 1242-1247
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2013 ◽
Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.
1979 ◽
Vol 76
(8)
◽
pp. 3755-3759
◽
Keyword(s):
1981 ◽
Vol 78
(3)
◽
pp. 1441-1445
◽
Keyword(s):
1981 ◽
Vol 38
(2)
◽
pp. 593-605
◽
Keyword(s):