scholarly journals Inhibition of protein kinase C zeta subspecies blocks the activation of an NF-kappa B-like activity in Xenopus laevis oocytes.

1993 ◽  
Vol 13 (2) ◽  
pp. 1290-1295 ◽  
Author(s):  
I Dominguez ◽  
L Sanz ◽  
F Arenzana-Seisdedos ◽  
M T Diaz-Meco ◽  
J L Virelizier ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Xenopus Laevis ◽  
Critical Role ◽  
Xenopus Laevis Oocytes ◽  
Nuclear Factor ◽  
Nf Kappa B ◽  
Kinase C ◽  
Ras P21 ◽  
Protein Kinase C Zeta

Nuclear factor kappa B (NF-kappa B) plays a critical role in the regulation of a large variety of cellular genes. However, the mechanism whereby this nuclear factor is activated remains to be determined. In this report, we present evidence that in oocytes from Xenopus laevis, (i) ras p21- and phospholipase C (PLC)-mediated phosphatidylcholine (PC) hydrolysis activates NF-kappa B and (ii) protein kinase C zeta subspecies is involved in the activation of NF-kappa B in response to insulin/ras p21/PC-PLC. Thus, the microinjection of either ras p21 or PC-PLC, or the exposure of oocytes to insulin, promotes a significant translocation to the nucleus of an NF-kappa B-like activity. This effect is not observed when oocytes are incubated with phorbol myristate acetate or progesterone, both of which utilize a ras p21-independent pathway for oocyte activation. These data strongly suggest a critical role of the insulin/ras p21/PC-PLC/protein kinase C zeta pathway in the control of NF-kappa B activation.

Inhibition of protein kinase C zeta subspecies blocks the activation of an NF-kappa B-like activity in Xenopus laevis oocytes

1993 ◽  
Vol 13 (2) ◽  
pp. 1290-1295
Author(s):  
I Dominguez ◽  
L Sanz ◽  
F Arenzana-Seisdedos ◽  
M T Diaz-Meco ◽  
J L Virelizier ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Xenopus Laevis ◽  
Critical Role ◽  
Xenopus Laevis Oocytes ◽  
Nuclear Factor ◽  
Nf Kappa B ◽  
Kinase C ◽  
Ras P21 ◽  
Protein Kinase C Zeta

Nuclear factor kappa B (NF-kappa B) plays a critical role in the regulation of a large variety of cellular genes. However, the mechanism whereby this nuclear factor is activated remains to be determined. In this report, we present evidence that in oocytes from Xenopus laevis, (i) ras p21- and phospholipase C (PLC)-mediated phosphatidylcholine (PC) hydrolysis activates NF-kappa B and (ii) protein kinase C zeta subspecies is involved in the activation of NF-kappa B in response to insulin/ras p21/PC-PLC. Thus, the microinjection of either ras p21 or PC-PLC, or the exposure of oocytes to insulin, promotes a significant translocation to the nucleus of an NF-kappa B-like activity. This effect is not observed when oocytes are incubated with phorbol myristate acetate or progesterone, both of which utilize a ras p21-independent pathway for oocyte activation. These data strongly suggest a critical role of the insulin/ras p21/PC-PLC/protein kinase C zeta pathway in the control of NF-kappa B activation.

scholarly journals Evidence for a role of protein kinase C zeta subspecies in maturation of Xenopus laevis oocytes.

1992 ◽  
Vol 12 (9) ◽  
pp. 3776-3783 ◽  
Author(s):  
I Dominguez ◽  
M T Diaz-Meco ◽  
M M Municio ◽  
E Berra ◽  
A García de Herreros ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Growth Factors ◽  
Protein Kinase ◽  
Xenopus Laevis ◽  
Critical Role ◽  
Study Data ◽  
Xenopus Laevis Oocytes ◽  
Kinase C ◽  
Protein Kinase C Zeta

A number of studies have demonstrated the activation of phospholipase C-mediated hydrolysis of phosphatidylcholine (PC-PLC) both by growth factors and by the product of the ras oncogene, p21ras. Evidence has been presented indicating that the stimulation of this phospholipid degradative pathway is sufficient to activate mitogenesis in fibroblasts as well as that it is sufficient and necessary for induction of maturation in Xenopus laevis oocytes. However, the mechanism whereby PC-PLC transduces mitogenic signals triggered by growth factors or oncogenes remains to be elucidated. In this study, data are presented that show the involvement of protein kinase C zeta subspecies in the channelling of the mitogenic signal activated by insulin-p21ras-PC-PLC in Xenopus oocytes as well as the lack of a critical role of protein kinase C isotypes alpha, beta, gamma, delta, and epsilon in these pathways.

Evidence for a role of protein kinase C zeta subspecies in maturation of Xenopus laevis oocytes

1992 ◽  
Vol 12 (9) ◽  
pp. 3776-3783
Author(s):  
I Dominguez ◽  
M T Diaz-Meco ◽  
M M Municio ◽  
E Berra ◽  
A García de Herreros ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Growth Factors ◽  
Protein Kinase ◽  
Xenopus Laevis ◽  
Critical Role ◽  
Study Data ◽  
Xenopus Laevis Oocytes ◽  
Kinase C ◽  
Protein Kinase C Zeta

A number of studies have demonstrated the activation of phospholipase C-mediated hydrolysis of phosphatidylcholine (PC-PLC) both by growth factors and by the product of the ras oncogene, p21ras. Evidence has been presented indicating that the stimulation of this phospholipid degradative pathway is sufficient to activate mitogenesis in fibroblasts as well as that it is sufficient and necessary for induction of maturation in Xenopus laevis oocytes. However, the mechanism whereby PC-PLC transduces mitogenic signals triggered by growth factors or oncogenes remains to be elucidated. In this study, data are presented that show the involvement of protein kinase C zeta subspecies in the channelling of the mitogenic signal activated by insulin-p21ras-PC-PLC in Xenopus oocytes as well as the lack of a critical role of protein kinase C isotypes alpha, beta, gamma, delta, and epsilon in these pathways.

scholarly journals A dominant negative protein kinase C zeta subspecies blocks NF-kappa B activation.

1993 ◽  
Vol 13 (8) ◽  
pp. 4770-4775 ◽  
Author(s):  
M T Diaz-Meco ◽  
E Berra ◽  
M M Municio ◽  
L Sanz ◽  
J Lozano ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Critical Role ◽  
Dominant Negative ◽  
Dominant Negative Mutant ◽  
Xenopus Laevis Oocytes ◽  
Nf Kappa B ◽  
3T3 Fibroblasts ◽  
Kinase C ◽  
Nih 3T3

Nuclear factor kappa B (NF-kappa B) plays a critical role in the regulation of a number of genes. NF-kappa B is a heterodimer of 50- and 65-kDa subunits sequestered in the cytoplasm complexed to inhibitory protein I kappa B. Following stimulation of cells, I kappa B dissociates from NF-kappa B, allowing its translocation to the nucleus, where it carries out the transactivation function. The precise mechanism controlling NF-kappa B activation and the involvement of members of the protein kinase C (PKC) family of isotypes have previously been investigated. It was found that phorbol myristate acetate, (PMA) which is a potent stimulant of phorbol ester-sensitive PKC isotypes, activates NF-kappa B. However, the role of PMA-sensitive PKCs in vivo is not as apparent. It has recently been demonstrated in the model system of Xenopus laevis oocytes that the PMA-insensitive PKC isotype, zeta PKC, is a required step in the activation of NF-kappa B in response to ras p21. We demonstrate here that overexpression of zeta PKC is by itself sufficient to stimulate a permanent translocation of functionally active NF-kappa B into the nucleus of NIH 3T3 fibroblasts and that transfection of a kinase-defective dominant negative mutant of zeta PKC dramatically inhibits the kappa B-dependent transactivation of a chloramphenicol acetyltransferase reporter plasmid in NIH 3T3 fibroblasts. All these results support the notion that zeta PKC plays a decisive role in NF-kappa B regulation in mammalian cells.

A dominant negative protein kinase C zeta subspecies blocks NF-kappa B activation

1993 ◽  
Vol 13 (8) ◽  
pp. 4770-4775
Author(s):  
M T Diaz-Meco ◽  
E Berra ◽  
M M Municio ◽  
L Sanz ◽  
J Lozano ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Critical Role ◽  
Dominant Negative ◽  
Dominant Negative Mutant ◽  
Xenopus Laevis Oocytes ◽  
Nf Kappa B ◽  
3T3 Fibroblasts ◽  
Kinase C ◽  
Nih 3T3

Nuclear factor kappa B (NF-kappa B) plays a critical role in the regulation of a number of genes. NF-kappa B is a heterodimer of 50- and 65-kDa subunits sequestered in the cytoplasm complexed to inhibitory protein I kappa B. Following stimulation of cells, I kappa B dissociates from NF-kappa B, allowing its translocation to the nucleus, where it carries out the transactivation function. The precise mechanism controlling NF-kappa B activation and the involvement of members of the protein kinase C (PKC) family of isotypes have previously been investigated. It was found that phorbol myristate acetate, (PMA) which is a potent stimulant of phorbol ester-sensitive PKC isotypes, activates NF-kappa B. However, the role of PMA-sensitive PKCs in vivo is not as apparent. It has recently been demonstrated in the model system of Xenopus laevis oocytes that the PMA-insensitive PKC isotype, zeta PKC, is a required step in the activation of NF-kappa B in response to ras p21. We demonstrate here that overexpression of zeta PKC is by itself sufficient to stimulate a permanent translocation of functionally active NF-kappa B into the nucleus of NIH 3T3 fibroblasts and that transfection of a kinase-defective dominant negative mutant of zeta PKC dramatically inhibits the kappa B-dependent transactivation of a chloramphenicol acetyltransferase reporter plasmid in NIH 3T3 fibroblasts. All these results support the notion that zeta PKC plays a decisive role in NF-kappa B regulation in mammalian cells.

scholarly journals Local anaesthetics inhibit signalling of human NMDA receptors recombinantly expressed in Xenopus laevis oocytes: role of protein kinase C

2006 ◽  
Vol 96 (1) ◽  
pp. 77-87 ◽  
Author(s):  
K Hahnenkamp ◽  
M.E. Durieux ◽  
A Hahnenkamp ◽  
S.K. Schauerte ◽  
C.W. Hoenemann ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Xenopus Laevis ◽  
Nmda Receptors ◽  
Local Anaesthetics ◽  
Xenopus Laevis Oocytes ◽  
Kinase C

scholarly journals Protein kinase C-zeta mediates NF-kappa B activation in human immunodeficiency virus-infected monocytes.

1996 ◽  
Vol 70 (1) ◽  
pp. 223-231 ◽  
Author(s):  
L Folgueira ◽  
J A McElhinny ◽  
G D Bren ◽  
W S MacMorran ◽  
M T Diaz-Meco ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Nf Kappa B ◽  
Kinase C ◽  
Immunodeficiency Virus ◽  
Protein Kinase C Zeta

Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes

FEBS Journal ◽  
2010 ◽  
Vol 277 (10) ◽  
pp. 2318-2328 ◽  
Author(s):  
Aiko Sato ◽  
Franco Gambale ◽  
Ingo Dreyer ◽  
Nobuyuki Uozumi
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Xenopus Laevis ◽  
Xenopus Laevis Oocytes ◽  
Kinase C
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