Protein kinase C activation blocks calcium receptor signaling in Xenopus laevis oocytes

A number of studies have demonstrated the activation of phospholipase C-mediated hydrolysis of phosphatidylcholine (PC-PLC) both by growth factors and by the product of the ras oncogene, p21ras. Evidence has been presented indicating that the stimulation of this phospholipid degradative pathway is sufficient to activate mitogenesis in fibroblasts as well as that it is sufficient and necessary for induction of maturation in Xenopus laevis oocytes. However, the mechanism whereby PC-PLC transduces mitogenic signals triggered by growth factors or oncogenes remains to be elucidated. In this study, data are presented that show the involvement of protein kinase C zeta subspecies in the channelling of the mitogenic signal activated by insulin-p21ras-PC-PLC in Xenopus oocytes as well as the lack of a critical role of protein kinase C isotypes alpha, beta, gamma, delta, and epsilon in these pathways.

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Local anaesthetics inhibit signalling of human NMDA receptors recombinantly expressed in Xenopus laevis oocytes: role of protein kinase C

British Journal of Anaesthesia ◽

10.1093/bja/aei271 ◽

2006 ◽

Vol 96 (1) ◽

pp. 77-87 ◽

Cited By ~ 62

Author(s):

K Hahnenkamp ◽

M.E. Durieux ◽

A Hahnenkamp ◽

S.K. Schauerte ◽

C.W. Hoenemann ◽

...

Keyword(s):

Protein Kinase C ◽

Protein Kinase ◽

Xenopus Laevis ◽

Nmda Receptors ◽

Local Anaesthetics ◽

Xenopus Laevis Oocytes ◽

Kinase C

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Evidence for a role of protein kinase C zeta subspecies in maturation of Xenopus laevis oocytes

Molecular and Cellular Biology ◽

10.1128/mcb.12.9.3776-3783.1992 ◽

1992 ◽

Vol 12 (9) ◽

pp. 3776-3783

Author(s):

I Dominguez ◽

M T Diaz-Meco ◽

M M Municio ◽

E Berra ◽

A García de Herreros ◽

...

Keyword(s):

Protein Kinase C ◽

Growth Factors ◽

Protein Kinase ◽

Xenopus Laevis ◽

Critical Role ◽

Study Data ◽

Xenopus Laevis Oocytes ◽

Kinase C ◽

Protein Kinase C Zeta

A number of studies have demonstrated the activation of phospholipase C-mediated hydrolysis of phosphatidylcholine (PC-PLC) both by growth factors and by the product of the ras oncogene, p21ras. Evidence has been presented indicating that the stimulation of this phospholipid degradative pathway is sufficient to activate mitogenesis in fibroblasts as well as that it is sufficient and necessary for induction of maturation in Xenopus laevis oocytes. However, the mechanism whereby PC-PLC transduces mitogenic signals triggered by growth factors or oncogenes remains to be elucidated. In this study, data are presented that show the involvement of protein kinase C zeta subspecies in the channelling of the mitogenic signal activated by insulin-p21ras-PC-PLC in Xenopus oocytes as well as the lack of a critical role of protein kinase C isotypes alpha, beta, gamma, delta, and epsilon in these pathways.

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Inhibition of protein kinase C zeta subspecies blocks the activation of an NF-kappa B-like activity in Xenopus laevis oocytes

Molecular and Cellular Biology ◽

10.1128/mcb.13.2.1290-1295.1993 ◽

1993 ◽

Vol 13 (2) ◽

pp. 1290-1295

Author(s):

I Dominguez ◽

L Sanz ◽

F Arenzana-Seisdedos ◽

M T Diaz-Meco ◽

J L Virelizier ◽

...

Keyword(s):

Protein Kinase C ◽

Protein Kinase ◽

Xenopus Laevis ◽

Critical Role ◽

Xenopus Laevis Oocytes ◽

Nuclear Factor ◽

Nf Kappa B ◽

Kinase C ◽

Ras P21 ◽

Protein Kinase C Zeta

Nuclear factor kappa B (NF-kappa B) plays a critical role in the regulation of a large variety of cellular genes. However, the mechanism whereby this nuclear factor is activated remains to be determined. In this report, we present evidence that in oocytes from Xenopus laevis, (i) ras p21- and phospholipase C (PLC)-mediated phosphatidylcholine (PC) hydrolysis activates NF-kappa B and (ii) protein kinase C zeta subspecies is involved in the activation of NF-kappa B in response to insulin/ras p21/PC-PLC. Thus, the microinjection of either ras p21 or PC-PLC, or the exposure of oocytes to insulin, promotes a significant translocation to the nucleus of an NF-kappa B-like activity. This effect is not observed when oocytes are incubated with phorbol myristate acetate or progesterone, both of which utilize a ras p21-independent pathway for oocyte activation. These data strongly suggest a critical role of the insulin/ras p21/PC-PLC/protein kinase C zeta pathway in the control of NF-kappa B activation.

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Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes

FEBS Journal ◽

10.1111/j.1742-4658.2010.07647.x ◽

2010 ◽

Vol 277 (10) ◽

pp. 2318-2328 ◽

Cited By ~ 22

Author(s):

Aiko Sato ◽

Franco Gambale ◽

Ingo Dreyer ◽

Nobuyuki Uozumi

Keyword(s):

Protein Kinase C ◽

Protein Kinase ◽

Xenopus Laevis ◽

Xenopus Laevis Oocytes ◽

Kinase C

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Protein kinase C consensus sites and the regulation of renal Na/Pi-cotransport (NaPi-2) expressed in XENOPUS laevis oocytes

Pflügers Archiv - European Journal of Physiology ◽

10.1007/bf00386181 ◽

1995 ◽

Vol 430 (5) ◽

pp. 819-824 ◽

Cited By ~ 34

Author(s):

Gillian Hayes ◽

Andreas E. Busch ◽

Florian Lang ◽

J�rg Biber ◽

Heini Murer

Keyword(s):

Protein Kinase C ◽

Protein Kinase ◽

Xenopus Laevis ◽

Xenopus Laevis Oocytes ◽

Kinase C

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Inhibition of protein kinase C zeta subspecies blocks the activation of an NF-kappa B-like activity in Xenopus laevis oocytes.

Molecular and Cellular Biology ◽

10.1128/mcb.13.2.1290 ◽

1993 ◽

Vol 13 (2) ◽

pp. 1290-1295 ◽

Cited By ~ 94

Author(s):

I Dominguez ◽

L Sanz ◽

F Arenzana-Seisdedos ◽

M T Diaz-Meco ◽

J L Virelizier ◽

...

Keyword(s):

Protein Kinase C ◽

Protein Kinase ◽

Xenopus Laevis ◽

Critical Role ◽

Xenopus Laevis Oocytes ◽

Nuclear Factor ◽

Nf Kappa B ◽

Kinase C ◽

Ras P21 ◽

Protein Kinase C Zeta

Nuclear factor kappa B (NF-kappa B) plays a critical role in the regulation of a large variety of cellular genes. However, the mechanism whereby this nuclear factor is activated remains to be determined. In this report, we present evidence that in oocytes from Xenopus laevis, (i) ras p21- and phospholipase C (PLC)-mediated phosphatidylcholine (PC) hydrolysis activates NF-kappa B and (ii) protein kinase C zeta subspecies is involved in the activation of NF-kappa B in response to insulin/ras p21/PC-PLC. Thus, the microinjection of either ras p21 or PC-PLC, or the exposure of oocytes to insulin, promotes a significant translocation to the nucleus of an NF-kappa B-like activity. This effect is not observed when oocytes are incubated with phorbol myristate acetate or progesterone, both of which utilize a ras p21-independent pathway for oocyte activation. These data strongly suggest a critical role of the insulin/ras p21/PC-PLC/protein kinase C zeta pathway in the control of NF-kappa B activation.

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