Selective Disruption of Genes Transiently Induced in Differentiating Mouse Embryonic Stem Cells by Using Gene Trap Mutagenesis and Site-Specific Recombination

1998 ◽  
Vol 18 (10) ◽  
pp. 6164-6164
Author(s):  
Irmgard S. Thorey ◽  
Katrin Muth ◽  
Andreas P. Russ ◽  
Jürgen Otte ◽  
Armin Reffelmann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gene Trap ◽  
Site Specific ◽  
Site Specific Recombination

scholarly journals Selective Disruption of Genes Transiently Induced in Differentiating Mouse Embryonic Stem Cells by Using Gene Trap Mutagenesis and Site-Specific Recombination

1998 ◽  
Vol 18 (5) ◽  
pp. 3081-3088 ◽  
Author(s):  
Irmgard S. Thorey ◽  
Katrin Muth ◽  
Andreas P. Russ ◽  
Jürgen Otte ◽  
Armin Reffelmann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Line ◽  
Embryonic Stem ◽  
Gene Trap ◽  
Marker Genes ◽  
Mouse Development ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Selectable Marker Genes

ABSTRACT A strategy employing gene trap mutagenesis and site-specific recombination (Cre/loxP) has been used to identify genes that are transiently expressed during early mouse development. Embryonic stem cells expressing a reporter plasmid that codes for neomycin phosphotransferase and Escherichia coli LacZ were infected with a retroviral gene trap vector (U3Cre) carrying coding sequences for Cre recombinase (Cre) in the U3 region. Activation of Cre expression from integrations into active genes resulted in a permanent switching between the two selectable marker genes and consequently the expression of β-galactosidase (β-Gal). As a result, clones in which U3Cre had disrupted genes that were only transiently expressed could be selected. Moreover, U3Cre-activating cells acquired a cell autonomous marker that could be traced to cells and tissues of the developing embryo. Thus, when two of the clones with inducible U3Cre integrations were passaged in the germ line, they generated spatial patterns of β-Gal expression.

scholarly journals Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis

2018 ◽  
Vol 46 (10) ◽  
pp. e63-e63
Author(s):  
Ayako Yamanishi ◽  
Atsushi Matsuba ◽  
Ryohei Kondo ◽  
Rie Akamatsu ◽  
Sachiyo Tanaka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mutant Mouse ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gene Trap ◽  
Homozygous Mutant ◽  
Homozygous Mutant Mouse

Site-specific recombination in human embryonic stem cells induced by cell-permeant Cre recombinase

2006 ◽  
Vol 3 (6) ◽  
pp. 461-467 ◽  
Author(s):  
Lars Nolden ◽  
Frank Edenhofer ◽  
Simone Haupt ◽  
Philipp Koch ◽  
F Thomas Wunderlich ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cre Recombinase ◽  
Site Specific ◽  
Site Specific Recombination

Engineering surfaces for site-specific vascular differentiation of mouse embryonic stem cells

2010 ◽  
Vol 6 (6) ◽  
pp. 1904-1916 ◽  
Author(s):  
C. Katherine Chiang ◽  
Mohammad Fahad Chowdhury ◽  
Rohin K. Iyer ◽  
William L. Stanford ◽  
Milica Radisic
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Vascular Differentiation ◽  
Site Specific
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