Less than 40% of the simian virus 40 large T-antigen-coding sequence is required for transformation

1984 ◽  
Vol 4 (8) ◽  
pp. 1661-1663
Author(s):  
L Sompayrac ◽  
K J Danna
Keyword(s):  
Dna Sequences ◽  
Deletion Mutant ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Large Deletion ◽  
T Antigen ◽  
Large T Antigen ◽  
Wild Type ◽  
Coding Sequence ◽  
Mouse Cells

F8dl is a simian virus 40 early-region deletion mutant that lacks the simian virus 40 DNA sequences between 0.168 and 0.424 map units. Despite this large deletion, cloned F8dl DNA transforms Fisher rat F111 cells and BALB/3T3 clone A31 mouse cells as efficiently as does cloned simian virus 40 wild-type DNA. These results indicate that less than 40% of the large T-antigen-coding sequence is required for efficient transformation.

scholarly journals Less than 40% of the simian virus 40 large T-antigen-coding sequence is required for transformation.

1984 ◽  
Vol 4 (8) ◽  
pp. 1661-1663 ◽  
Author(s):  
L Sompayrac ◽  
K J Danna
Keyword(s):  
Dna Sequences ◽  
Deletion Mutant ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Large Deletion ◽  
T Antigen ◽  
Large T Antigen ◽  
Wild Type ◽  
Coding Sequence ◽  
Mouse Cells

F8dl is a simian virus 40 early-region deletion mutant that lacks the simian virus 40 DNA sequences between 0.168 and 0.424 map units. Despite this large deletion, cloned F8dl DNA transforms Fisher rat F111 cells and BALB/3T3 clone A31 mouse cells as efficiently as does cloned simian virus 40 wild-type DNA. These results indicate that less than 40% of the large T-antigen-coding sequence is required for efficient transformation.

scholarly journals Excess wild-type p53 blocks initiation and maintenance of simian virus 40 transformation.

1991 ◽  
Vol 11 (7) ◽  
pp. 3472-3483 ◽  
Author(s):  
K Fukasawa ◽  
G Sakoulas ◽  
R E Pollack ◽  
S Chen
Keyword(s):  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Wild Type ◽  
Sv40 Large T Antigen ◽  
Sv40 Transformation ◽  
Mouse Cells ◽  
Wild Type P53

Wild-type (wt) murine p53 has been tested for its ability to block and reverse the transforming effects of simian virus 40 (SV40) large T antigen. Established and precrisis mouse cells overexpressing exogenously introduced wt p53 became resistant to SV40 transformation. The introduction of excess wt p53 into SV40-transformed precrisis cells reverted their transformed phenotype. However, the phenotype of SV40-transformed established cells was not reverted by excess wt p53. We conclude that an antioncogenic action of wt p53 is exerted during SV40 transformation and that in precrisis cells, the antitransforming action of wt p53 can be exerted both at initiation and during the maintenance of transformation.

Excess wild-type p53 blocks initiation and maintenance of simian virus 40 transformation

1991 ◽  
Vol 11 (7) ◽  
pp. 3472-3483
Author(s):  
K Fukasawa ◽  
G Sakoulas ◽  
R E Pollack ◽  
S Chen
Keyword(s):  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Wild Type ◽  
Sv40 Large T Antigen ◽  
Sv40 Transformation ◽  
Mouse Cells ◽  
Wild Type P53

Wild-type (wt) murine p53 has been tested for its ability to block and reverse the transforming effects of simian virus 40 (SV40) large T antigen. Established and precrisis mouse cells overexpressing exogenously introduced wt p53 became resistant to SV40 transformation. The introduction of excess wt p53 into SV40-transformed precrisis cells reverted their transformed phenotype. However, the phenotype of SV40-transformed established cells was not reverted by excess wt p53. We conclude that an antioncogenic action of wt p53 is exerted during SV40 transformation and that in precrisis cells, the antitransforming action of wt p53 can be exerted both at initiation and during the maintenance of transformation.

scholarly journals Stabilization of the 53,000-dalton nonviral tumor antigen is not required for transformation by simian virus 40.

1983 ◽  
Vol 3 (2) ◽  
pp. 290-296 ◽  
Author(s):  
L M Sompayrac ◽  
E G Gurney ◽  
K J Danna
Keyword(s):  
Cell Lines ◽  
Tumor Antigen ◽  
Deletion Mutant ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Coding Region ◽  
Present Evidence ◽  
Mouse Cells

We have isolated a simian virus 40 deletion mutant, F8dl, that lacks the sequences from 0.168 to 0.424 map units. The deleted sequences represent about one-half of the coding region for large T antigen. We present evidence here that F8dl is able to transform mouse cells in a focus assay and that cell lines derived from these foci exhibit fully transformed phenotypes, have integrated mutant genomes, and express mutant-encoded proteins. This result implies that the region of the simian virus 40 genome between 0.168 and 0.424 map units is not essential for the maintenance of transformation. In addition, we have found that cells fully transformed by F8dl produce a 53,000-dalton nonviral tumor antigen (p53) that is as unstable as the p53 of untransformed cells. From this result we infer that transformation by simian virus 40 does not require the stabilization of p53.

Stabilization of the 53,000-dalton nonviral tumor antigen is not required for transformation by simian virus 40

1983 ◽  
Vol 3 (2) ◽  
pp. 290-296
Author(s):  
L M Sompayrac ◽  
E G Gurney ◽  
K J Danna
Keyword(s):  
Cell Lines ◽  
Tumor Antigen ◽  
Deletion Mutant ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Coding Region ◽  
Present Evidence ◽  
Mouse Cells

We have isolated a simian virus 40 deletion mutant, F8dl, that lacks the sequences from 0.168 to 0.424 map units. The deleted sequences represent about one-half of the coding region for large T antigen. We present evidence here that F8dl is able to transform mouse cells in a focus assay and that cell lines derived from these foci exhibit fully transformed phenotypes, have integrated mutant genomes, and express mutant-encoded proteins. This result implies that the region of the simian virus 40 genome between 0.168 and 0.424 map units is not essential for the maintenance of transformation. In addition, we have found that cells fully transformed by F8dl produce a 53,000-dalton nonviral tumor antigen (p53) that is as unstable as the p53 of untransformed cells. From this result we infer that transformation by simian virus 40 does not require the stabilization of p53.

Separation of simian virus 40 large-T-antigen-transforming and origin-binding functions from the ability to block differentiation

1988 ◽  
Vol 8 (3) ◽  
pp. 1380-1384 ◽  
Author(s):  
V Cherington ◽  
M Brown ◽  
E Paucha ◽  
J St Louis ◽  
B M Spiegelman ◽  
...  
Keyword(s):  
Dehydrogenase Activity ◽  
Adipocyte Differentiation ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Lipid Binding ◽  
T Antigen ◽  
Large T Antigen ◽  
Wild Type ◽  
Glycerophosphate Dehydrogenase ◽  
Triglyceride Accumulation

Wild-type simian virus 40 large T antigen is very effective at blocking adipocyte differentiation in 3T3-F442A cells as assayed by triglyceride accumulation, induction of glycerophosphate dehydrogenase activity, and expression of mRNAs for glycerophosphate dehydrogenase, the adipocyte serine protease adipsin, and the putative lipid-binding protein adipocyte P2. Point mutants defective for either origin-specific DNA binding or transformation blocked differentiation as completely as wild type.

scholarly journals New Insights into the Mechanism of Inhibition of p53 by Simian Virus 40 Large T Antigen

1999 ◽  
Vol 19 (4) ◽  
pp. 2746-2753 ◽  
Author(s):  
Hilary M. Sheppard ◽  
Siska I. Corneillie ◽  
Christine Espiritu ◽  
Andrea Gatti ◽  
Xuan Liu
Keyword(s):  
Tumor Antigen ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Human Cells ◽  
Transactivation Domain ◽  
Large T Antigen ◽  
Large Tumor ◽  
Mechanism Of Inhibition ◽  
Mouse Cells

ABSTRACT Simian virus 40 (SV40) large tumor antigen (T antigen) has been shown to inhibit p53-dependent transcription by preventing p53 from binding to its cognate cis element. Data presented in this report provide the first direct functional evidence that T antigen, under certain conditions, may also repress p53-dependent transcription by a mechanism in which the transactivation domain of p53 is abrogated while DNA binding is unaffected. Specifically, p53 purified as a complex with T antigen from mouse cells was found to bind DNA as a transcriptionally inactive intact complex, while that purified from human cells was found to bind DNA independently of T antigen and could activate p53-dependent transcription. This difference in activity may be dependent on a different interaction of T antigen with mouse and human p53 and, in addition, on the presence of super T, which is found only in transformed rodent cells. These results suggest that subtle yet important differences exist between the inhibition of p53 by T antigen in mouse and human cells. The implications of this finding with respect to SV40-associated malignancies are discussed.

scholarly journals Separation of simian virus 40 large-T-antigen-transforming and origin-binding functions from the ability to block differentiation.

1988 ◽  
Vol 8 (3) ◽  
pp. 1380-1384 ◽  
Author(s):  
V Cherington ◽  
M Brown ◽  
E Paucha ◽  
J St Louis ◽  
B M Spiegelman ◽  
...  
Keyword(s):  
Dehydrogenase Activity ◽  
Adipocyte Differentiation ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Lipid Binding ◽  
T Antigen ◽  
Large T Antigen ◽  
Wild Type ◽  
Glycerophosphate Dehydrogenase ◽  
Triglyceride Accumulation

Wild-type simian virus 40 large T antigen is very effective at blocking adipocyte differentiation in 3T3-F442A cells as assayed by triglyceride accumulation, induction of glycerophosphate dehydrogenase activity, and expression of mRNAs for glycerophosphate dehydrogenase, the adipocyte serine protease adipsin, and the putative lipid-binding protein adipocyte P2. Point mutants defective for either origin-specific DNA binding or transformation blocked differentiation as completely as wild type.

scholarly journals Replication but Not Transcription of Simian Virus 40 DNA Is Dependent on Nuclear Domain 10

2000 ◽  
Vol 74 (20) ◽  
pp. 9694-9700 ◽  
Author(s):  
Qiyi Tang ◽  
Peter Bell ◽  
Peter Tegtmeyer ◽  
Gerd G. Maul
Keyword(s):  
Simian Virus 40 ◽  
Adenovirus Type ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Antigen Binding Site ◽  
Viral Origin ◽  
Coding Sequence ◽  
Nuclear Domain ◽  
Transcription And Replication

ABSTRACT DNA viruses from several families including herpes simplex virus type 1, adenovirus type 5, and simian virus 40 (SV40), start their transcription and replication adjacent to a specific nuclear domain, ND10. We asked whether a specific viral DNA sequence determines the location of these synthetic activities at such restricted nuclear sites. Partial and overlapping SV40 sequences were introduced into a β-galactosidase expression vector, and the β-galactosidase transcripts were localized by in situ hybridization. Transcripts derived from control plasmids were found throughout the nucleus and at highly concentrated sites but not at ND10. SV40 genomic segments supported ND10-associated transcription only when the origin and the coding sequence for the large T antigen were present. When the large T-antigen coding sequence was eliminated but the T antigen was constitutively expressed in COS-7 cells, the viral origin was sufficient to localize transcription and replication to ND10. Deletion analysis showed that only the large T-antigen binding site II (the core origin) was required but the T antigen was needed for detectable transcription at ND10. Large T antigen expressed from plasmids without the viral core origin did not bind or localize to ND10. Blocking of DNA replication prevented the accumulation of transcripts at ND10, indicating that only sites with replicating templates accumulated transcripts. Transcription at ND10 did not enhance total protein synthesis of plasmid transcripts. These findings suggest that viral transcription at ND10 may only be a consequence of viral genomes directed to ND10 for replication. Although plasmid transcription can take place anywhere in the nucleus, T-antigen-directed replication is apparently restricted to ND10.

scholarly journals Autonomous replicating sequences from mouse cells which can replicate in mouse cells in vivo and in vitro.

1987 ◽  
Vol 7 (1) ◽  
pp. 1-6 ◽  
Author(s):  
H Ariga ◽  
T Itani ◽  
S M Iguchi-Ariga
Keyword(s):  
Dna Replication ◽  
Dna Sequences ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Copy Numbers ◽  
Mouse Cells ◽  
Simplex Virus

We have already reported that the cloned mouse DNA fragment (pMU65) could replicate in a simian virus 40 T antigen-dependent system in vivo and in vitro (H. Ariga, Z. Tsuchihashi, M. Naruto, and M. Yamada, Mol. Cell. Biol. 5:563-568, 1985). The plasmid p65-tk, containing the thymidine kinase (tk) gene of herpes simplex virus and the BglII-EcoRI region of pMU65 homologous to the simian virus 40 origin of DNA replication, was constructed. The p65-tk persisted episomally in tk+ transformants after the transfection of p65-tk into mouse FM3Atk- cells. The copy numbers of p65-tk in FM3Atk+ cells were 100 to 200 copies per cell. Furthermore, the p65-tk replicated semiconservatively, and the initiation of DNA replication started from the mouse DNA sequences when the replicating activity of p65-tk was tested in the in vitro DNA replication system developed from the FM3A cells. These results show that a 2.5-kilobase fragment of mouse DNA contains the autonomously replicating sequences.

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