Heat-inducible human factor that binds to a human hsp70 promoter

1987 ◽  
Vol 7 (4) ◽  
pp. 1530-1534
Author(s):  
R E Kingston ◽  
T J Schuetz ◽  
Z Larin
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein 70 ◽  
Human Factor ◽  
Regulatory Factor ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
Initial Event ◽  
Nuclear Extracts ◽  
Human Heat Shock Protein ◽  
Human Hsp70

A factor found in nuclear extracts of human cells bound to the heat shock element of a human heat shock protein 70 gene. The level of this factor was significantly increased after heat shock. This induction was rapid and was not blocked by cycloheximide, suggesting that an initial event in the response of a human cell to heat is the activation of a preexisting regulatory factor.

scholarly journals Heat-inducible human factor that binds to a human hsp70 promoter.

1987 ◽  
Vol 7 (4) ◽  
pp. 1530-1534 ◽  
Author(s):  
R E Kingston ◽  
T J Schuetz ◽  
Z Larin
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein 70 ◽  
Human Factor ◽  
Regulatory Factor ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
Initial Event ◽  
Nuclear Extracts ◽  
Human Heat Shock Protein ◽  
Human Hsp70

A factor found in nuclear extracts of human cells bound to the heat shock element of a human heat shock protein 70 gene. The level of this factor was significantly increased after heat shock. This induction was rapid and was not blocked by cycloheximide, suggesting that an initial event in the response of a human cell to heat is the activation of a preexisting regulatory factor.

TATA-dependent and TATA-independent function of the basal and heat shock elements of a human hsp70 promoter

1990 ◽  
Vol 10 (4) ◽  
pp. 1319-1328
Author(s):  
J M Greene ◽  
R E Kingston
Keyword(s):  
Heat Shock ◽  
Consensus Sequence ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
Tata Element ◽  
Sequence Elements ◽  
Human Heat Shock Protein ◽  
Human Hsp70

We have characterized the interactions between the TATA element and other sequence elements of a human heat shock protein 70 (hsp70) promoter by a mutational approach. Expression of a distal element of this promoter requires an intact TATA element in human cell lines. The hsp70 TATA element can be functionally replaced for this interaction by TATA elements from the simian virus 40 early and adenovirus EIIa promoters. The TATA element in this promoter therefore both determines the appropriate start site and determines strength by allowing function of the distal element. In contrast, three proximal upstream elements necessary for basal and heat-regulated transcription have no requirement either for a TATA element or for any other proximal element. The behavior of promoters multiply mutant in these proximal elements implies that these elements function independently. We examined the interaction between the heat shock element (HSE) and the TATA element as the distance between the two factor-binding sites was increased. It was necessary to create a mutant HSE with an extended consensus sequence in order for the HSE to function at a distance. Moving this extended HSE 500 bases upstream did not increase its dependence on the TATA element, suggesting that the TATA independence of this element is intrinsic to its function and is not determined by distance from the promoter.

scholarly journals TATA-dependent and TATA-independent function of the basal and heat shock elements of a human hsp70 promoter.

1990 ◽  
Vol 10 (4) ◽  
pp. 1319-1328 ◽  
Author(s):  
J M Greene ◽  
R E Kingston
Keyword(s):  
Heat Shock ◽  
Consensus Sequence ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
Tata Element ◽  
Sequence Elements ◽  
Human Heat Shock Protein ◽  
Human Hsp70

We have characterized the interactions between the TATA element and other sequence elements of a human heat shock protein 70 (hsp70) promoter by a mutational approach. Expression of a distal element of this promoter requires an intact TATA element in human cell lines. The hsp70 TATA element can be functionally replaced for this interaction by TATA elements from the simian virus 40 early and adenovirus EIIa promoters. The TATA element in this promoter therefore both determines the appropriate start site and determines strength by allowing function of the distal element. In contrast, three proximal upstream elements necessary for basal and heat-regulated transcription have no requirement either for a TATA element or for any other proximal element. The behavior of promoters multiply mutant in these proximal elements implies that these elements function independently. We examined the interaction between the heat shock element (HSE) and the TATA element as the distance between the two factor-binding sites was increased. It was necessary to create a mutant HSE with an extended consensus sequence in order for the HSE to function at a distance. Moving this extended HSE 500 bases upstream did not increase its dependence on the TATA element, suggesting that the TATA independence of this element is intrinsic to its function and is not determined by distance from the promoter.

scholarly journals B-Myb and cyclin D1 mediate heat shock element dependent activation of the human HSP70 promoter

Oncogene ◽  
1997 ◽  
Vol 14 (10) ◽  
pp. 1223-1229 ◽  
Author(s):  
Hiroshi Kamano ◽  
Karl-Heinz Klempnauer
Keyword(s):  
Heat Shock ◽  
Cyclin D1 ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
Human Hsp70

Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting

1991 ◽  
Vol 11 (1) ◽  
pp. 586-592
Author(s):  
K Abravaya ◽  
B Phillips ◽  
R I Morimoto
Keyword(s):  
Transcription Factor ◽  
Heat Shock ◽  
Consensus Sequence ◽  
Regulatory Elements ◽  
Heat Shock Transcription Factor ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
In Vivo Footprinting ◽  
Human Hsp70

Genomic footprinting of the human hsp70 promoter reveals that heat shock induces a rapid binding of a factor, presumably heat shock transcription factor, to a region encompassing five contiguous NGAAN sequences, three perfect and two imperfect matches to the consensus sequence. Arrays of inverted NGAAN sequences have been defined as the heat shock element. No protein is bound to the heat shock element prior to or after recovery from heat shock. Heat shock does not perturb the binding of factors to other regulatory elements in the promoter which contribute to basal expression of the hsp70 gene.

scholarly journals Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting.

1991 ◽  
Vol 11 (1) ◽  
pp. 586-592 ◽  
Author(s):  
K Abravaya ◽  
B Phillips ◽  
R I Morimoto
Keyword(s):  
Transcription Factor ◽  
Heat Shock ◽  
Consensus Sequence ◽  
Regulatory Elements ◽  
Heat Shock Transcription Factor ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
In Vivo Footprinting ◽  
Human Hsp70

Genomic footprinting of the human hsp70 promoter reveals that heat shock induces a rapid binding of a factor, presumably heat shock transcription factor, to a region encompassing five contiguous NGAAN sequences, three perfect and two imperfect matches to the consensus sequence. Arrays of inverted NGAAN sequences have been defined as the heat shock element. No protein is bound to the heat shock element prior to or after recovery from heat shock. Heat shock does not perturb the binding of factors to other regulatory elements in the promoter which contribute to basal expression of the hsp70 gene.

scholarly journals Stimulation of the Human Heat Shock Protein 70 Promoter in Vitro by Simian Virus 40 Large T Antigen

1989 ◽  
Vol 264 (27) ◽  
pp. 16160-16164
Author(s):  
I C Taylor ◽  
W Solomon ◽  
B M Weiner ◽  
E Paucha ◽  
M Bradley ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Human Heat Shock Protein ◽  
Stimulation Of

Two transcriptional activators, CCAAT-box-binding transcription factor and heat shock transcription factor, interact with a human hsp70 gene promoter

1987 ◽  
Vol 7 (3) ◽  
pp. 1129-1138
Author(s):  
W D Morgan ◽  
G T Williams ◽  
R I Morimoto ◽  
J Greene ◽  
R E Kingston ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Heat Shock ◽  
Gene Promoter ◽  
Heat Shock Transcription Factor ◽  
Transcriptional Activators ◽  
Hsp70 Gene ◽  
Nuclear Extracts ◽  
Ccaat Box ◽  
Human Hsp70

We characterized the activity of a human hsp70 gene promoter by in vitro transcription. Analysis of 5' deletion and substitution mutants in HeLa nuclear extracts showed that the basal activity of the promoter depends primarily on a CCAAT-box sequence located at -65. A protein factor, CCAAT-box-binding transcription factor (CTF), was isolated from HeLa nuclear extracts and shown to be responsible for stimulation of transcription in a reconstituted in vitro system. DNase I footprinting revealed that CTF interacts with two CCAAT-box elements located at -65 and -147 of the human hsp70 promoter. An additional binding activity, heat shock transcription factor (HSTF), which interacted with the heat shock element, was also identified in HeLa extract fractions. This demonstrates that the promoter of this human hsp70 gene interacts with at least two positive transcriptional activators, CTF, which is required for CCAAT-box-dependent transcription as in other promoters such as those of globin and herpes simplex virus thymidine kinase genes, and HSTF, which is involved in heat inducibility.

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