in vivo footprinting
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2017 ◽  
Vol 71 (0) ◽  
pp. 0-0
Author(s):  
Joanna Hołówka ◽  
Małgorzata Płachetka

According to recent reports, bacterial chromosomes exhibit a hierarchical organization. The number of proteins that bind DNA are responsible for local and global organization of the DNA ensuring proper chromosome compaction. Advanced molecular biology techniques combined with high-throughput DNA sequencing methods allow a precise analysis of bacterial chromosome structures on a local and global scale. Methods such as in vivo footprinting and ChIP-seq allow to map binding sites of analyzed proteins in certain chromosomal regions or along the whole chromosome while analysis of the spatial interactions on global scale could be performed by 3C techniques. Additional insight into complex structures created by chromosome-organizing proteins is provided by high-resolution fluorescence microscopy techniques.



2013 ◽  
Vol 42 (1) ◽  
pp. e1-e1 ◽  
Author(s):  
Rita Gorsche ◽  
Birgit Jovanovic ◽  
Loreta Gudynaite-Savitch ◽  
Robert L. Mach ◽  
Astrid R. Mach-Aigner


2010 ◽  
Vol 21 (3) ◽  
pp. 456-464 ◽  
Author(s):  
A. P. Boyle ◽  
L. Song ◽  
B.-K. Lee ◽  
D. London ◽  
D. Keefe ◽  
...  


2009 ◽  
Vol 29 (20) ◽  
pp. 5413-5425 ◽  
Author(s):  
Sergey Belikov ◽  
Carolina Åstrand ◽  
Örjan Wrange

ABSTRACT Reconstitution of the glucocorticoid receptor (GR)-regulated mouse mammary tumor virus (MMTV) promoter in Xenopus oocytes was used to monitor the effects of different transcription factor contexts. Three constitutively binding factors, nuclear factor 1 (NF1), octamer transcription factor 1 (Oct1), and the Forkhead box A1 (FoxA1), were shown to act in concert, to direct the chromatin structure, and to enhance the GR response. FoxA1 has a dominant effect in the absence of hormone and induces a cluster of DNase I-hypersensitive sites in the segment comprising bp −400 to +25. This FoxA1-mediated chromatin remodeling does not induce MMTV transcription, as opposed to that of the GR. However, the robust FoxA1-dependent chromatin opening has the following drastic functional consequences on the hormone regulation: (i) GR-DNA binding is facilitated, as revealed by dimethyl sulfate in vivo footprinting, leading to increased hormone-induced transcription, and (ii) the GR antagonist RU486 is converted into a partial agonist in the presence of FoxA1 via ligand-independent GR activation. We conclude that FoxA1 mediates a preset chromatin structure and directs a context-specific response of a nuclear receptor. Furthermore, the alternative nucleosome arrangement induced by GR and FoxA1 implies this to be determined by constitutive binding of transcription factors rather than by the DNA sequence itself.



Author(s):  
Gino Boily ◽  
Stéphane Ouellet ◽  
Sylvie Langlois ◽  
Mathieu Larivière ◽  
Régen Drouin ◽  
...  




2006 ◽  
Vol 5 (3) ◽  
pp. 447-456 ◽  
Author(s):  
Roman Rauscher ◽  
Elisabeth Würleitner ◽  
Christian Wacenovsky ◽  
Nina Aro ◽  
Astrid R. Stricker ◽  
...  

ABSTRACT Two major xylanases (XYN I and XYN II) of the filamentous fungus Hypocrea jecorina (Trichoderma reesei) are simultaneously expressed during growth on xylan but respond differently to low-molecular-weight inducers. In vivo footprinting analysis of the xylanase1 (xyn1) promoter revealed three different nucleotide sequences (5′- GGCTAA ATGCGACATC TTAGCC -3′ [an inverted repeat of GGCTAA spaced by 10 bp], 5′-CCAAT-3′, and 5′- GGGGTC TA GACCCC -3′ [equivalent to a double Cre1 site]) used to bind proteins. Binding to the Cre1 site is only observed under repressed conditions, whereas binding to the two other motifs is constitutive. Applying heterologously expressed components of the H. jecorina cellulase regulators Ace1 and Ace2 and the xylanase regulator Xyr1 suggests that Ace1 and Xyr1 but not Ace2 contact both GGCTAA motifs. H. jecorina transformants containing mutated versions of the xyn1 promoter, leading to elimination of protein binding to the left or the right GGCTAA box revealed either strongly reduced or completely eliminated induction of transcription. Elimination of Cre1 binding to its target released the basal transcriptional level from glucose repression but did not influence the inducibility of xyn1 expression. Mutation of the CCAAT box prevents binding of the Hap2/3/5 complex in vitro and is partially compensating for the loss of transcription caused by the mutation of the right GGCTAA box. Finally, evidence for a competition of Ace1 and Xyr1 for the right GGCTAA box is given. These data prompted us to hypothesize that xyn1 regulation is based on the interplay of Cre1 and Ace1 as a general and specific repressor with Xyr1 as transactivator.



2005 ◽  
Vol 94 (5) ◽  
pp. 1046-1057 ◽  
Author(s):  
Meredith L. Hanel ◽  
Jason C.Y. Lau ◽  
Isabelle Paradis ◽  
R�gen Drouin ◽  
Rachel Wevrick


2004 ◽  
Vol 383 (2) ◽  
pp. 209-218 ◽  
Author(s):  
Thomas D. HOCK ◽  
Harry S. NICK ◽  
Anupam AGARWAL

The human HO-1 (haem oxygenase-1) gene encodes a microsomal enzyme responsible for the breakdown of haem, and is also cytoprotective in response to various cellular insults. HO-1 transcription is induced by a vast array of compounds including, but certainly not limited to, haem and heavy metals such as cadmium. In the present study, we show that upstream stimulatory factors, USF1 and USF2, ubiquitous proteins belonging to the basic helix–loop–helix-leucine zipper family of transcription factors, constitutively bind to the class B E-box located in the proximal promoter of the human HO-1 gene and are responsible for the enhancement of HO-1 gene transcription in human renal proximal tubular epithelial cells. Dimethylsulphate in vivo footprinting studies have identified three protected guanine residues in the E-box of the HO-1 proximal promoter. One of these guanine contact points is essential for USF binding, and when mutated mimics a deletion mutation of the entire E-box palindrome sequence encompassing all three guanine contact points. Binding of USF1 and USF2 to the HO-1 E-box was confirmed by chromatin immunoprecipitation and gel-shift assays. Furthermore, we show that overexpression of USF1 or USF2 enhances the basal expression of HO-1 and that expression of a USF dominant negative form reduces its expression. These results demonstrate for the first time that USF proteins bind to the human HO-1 promoter in vivo and are required for high-level expression of HO-1 by haem and cadmium in human renal epithelial cells.



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