TATA-dependent and TATA-independent function of the basal and heat shock elements of a human hsp70 promoter

1990 ◽  
Vol 10 (4) ◽  
pp. 1319-1328
Author(s):  
J M Greene ◽  
R E Kingston
Keyword(s):  
Heat Shock ◽  
Consensus Sequence ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
Tata Element ◽  
Sequence Elements ◽  
Human Heat Shock Protein ◽  
Human Hsp70

We have characterized the interactions between the TATA element and other sequence elements of a human heat shock protein 70 (hsp70) promoter by a mutational approach. Expression of a distal element of this promoter requires an intact TATA element in human cell lines. The hsp70 TATA element can be functionally replaced for this interaction by TATA elements from the simian virus 40 early and adenovirus EIIa promoters. The TATA element in this promoter therefore both determines the appropriate start site and determines strength by allowing function of the distal element. In contrast, three proximal upstream elements necessary for basal and heat-regulated transcription have no requirement either for a TATA element or for any other proximal element. The behavior of promoters multiply mutant in these proximal elements implies that these elements function independently. We examined the interaction between the heat shock element (HSE) and the TATA element as the distance between the two factor-binding sites was increased. It was necessary to create a mutant HSE with an extended consensus sequence in order for the HSE to function at a distance. Moving this extended HSE 500 bases upstream did not increase its dependence on the TATA element, suggesting that the TATA independence of this element is intrinsic to its function and is not determined by distance from the promoter.

scholarly journals TATA-dependent and TATA-independent function of the basal and heat shock elements of a human hsp70 promoter.

1990 ◽  
Vol 10 (4) ◽  
pp. 1319-1328 ◽  
Author(s):  
J M Greene ◽  
R E Kingston
Keyword(s):  
Heat Shock ◽  
Consensus Sequence ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
Tata Element ◽  
Sequence Elements ◽  
Human Heat Shock Protein ◽  
Human Hsp70

We have characterized the interactions between the TATA element and other sequence elements of a human heat shock protein 70 (hsp70) promoter by a mutational approach. Expression of a distal element of this promoter requires an intact TATA element in human cell lines. The hsp70 TATA element can be functionally replaced for this interaction by TATA elements from the simian virus 40 early and adenovirus EIIa promoters. The TATA element in this promoter therefore both determines the appropriate start site and determines strength by allowing function of the distal element. In contrast, three proximal upstream elements necessary for basal and heat-regulated transcription have no requirement either for a TATA element or for any other proximal element. The behavior of promoters multiply mutant in these proximal elements implies that these elements function independently. We examined the interaction between the heat shock element (HSE) and the TATA element as the distance between the two factor-binding sites was increased. It was necessary to create a mutant HSE with an extended consensus sequence in order for the HSE to function at a distance. Moving this extended HSE 500 bases upstream did not increase its dependence on the TATA element, suggesting that the TATA independence of this element is intrinsic to its function and is not determined by distance from the promoter.

Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting

1991 ◽  
Vol 11 (1) ◽  
pp. 586-592
Author(s):  
K Abravaya ◽  
B Phillips ◽  
R I Morimoto
Keyword(s):  
Transcription Factor ◽  
Heat Shock ◽  
Consensus Sequence ◽  
Regulatory Elements ◽  
Heat Shock Transcription Factor ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
In Vivo Footprinting ◽  
Human Hsp70

Genomic footprinting of the human hsp70 promoter reveals that heat shock induces a rapid binding of a factor, presumably heat shock transcription factor, to a region encompassing five contiguous NGAAN sequences, three perfect and two imperfect matches to the consensus sequence. Arrays of inverted NGAAN sequences have been defined as the heat shock element. No protein is bound to the heat shock element prior to or after recovery from heat shock. Heat shock does not perturb the binding of factors to other regulatory elements in the promoter which contribute to basal expression of the hsp70 gene.

Heat-inducible human factor that binds to a human hsp70 promoter

1987 ◽  
Vol 7 (4) ◽  
pp. 1530-1534
Author(s):  
R E Kingston ◽  
T J Schuetz ◽  
Z Larin
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein 70 ◽  
Human Factor ◽  
Regulatory Factor ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
Initial Event ◽  
Nuclear Extracts ◽  
Human Heat Shock Protein ◽  
Human Hsp70

A factor found in nuclear extracts of human cells bound to the heat shock element of a human heat shock protein 70 gene. The level of this factor was significantly increased after heat shock. This induction was rapid and was not blocked by cycloheximide, suggesting that an initial event in the response of a human cell to heat is the activation of a preexisting regulatory factor.

scholarly journals Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting.

1991 ◽  
Vol 11 (1) ◽  
pp. 586-592 ◽  
Author(s):  
K Abravaya ◽  
B Phillips ◽  
R I Morimoto
Keyword(s):  
Transcription Factor ◽  
Heat Shock ◽  
Consensus Sequence ◽  
Regulatory Elements ◽  
Heat Shock Transcription Factor ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
In Vivo Footprinting ◽  
Human Hsp70

Genomic footprinting of the human hsp70 promoter reveals that heat shock induces a rapid binding of a factor, presumably heat shock transcription factor, to a region encompassing five contiguous NGAAN sequences, three perfect and two imperfect matches to the consensus sequence. Arrays of inverted NGAAN sequences have been defined as the heat shock element. No protein is bound to the heat shock element prior to or after recovery from heat shock. Heat shock does not perturb the binding of factors to other regulatory elements in the promoter which contribute to basal expression of the hsp70 gene.

scholarly journals Heat-inducible human factor that binds to a human hsp70 promoter.

1987 ◽  
Vol 7 (4) ◽  
pp. 1530-1534 ◽  
Author(s):  
R E Kingston ◽  
T J Schuetz ◽  
Z Larin
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein 70 ◽  
Human Factor ◽  
Regulatory Factor ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
Initial Event ◽  
Nuclear Extracts ◽  
Human Heat Shock Protein ◽  
Human Hsp70

A factor found in nuclear extracts of human cells bound to the heat shock element of a human heat shock protein 70 gene. The level of this factor was significantly increased after heat shock. This induction was rapid and was not blocked by cycloheximide, suggesting that an initial event in the response of a human cell to heat is the activation of a preexisting regulatory factor.

scholarly journals Stimulation of the Human Heat Shock Protein 70 Promoter in Vitro by Simian Virus 40 Large T Antigen

1989 ◽  
Vol 264 (27) ◽  
pp. 16160-16164
Author(s):  
I C Taylor ◽  
W Solomon ◽  
B M Weiner ◽  
E Paucha ◽  
M Bradley ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Human Heat Shock Protein ◽  
Stimulation Of

Organization of the Drosophila melanogaster hsp70 heat shock regulation unit

1987 ◽  
Vol 7 (3) ◽  
pp. 1055-1062
Author(s):  
J Amin ◽  
R Mestril ◽  
P Schiller ◽  
M Dreano ◽  
R Voellmy
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Synergistic Effect ◽  
Consensus Sequence ◽  
Relative Orientation ◽  
Hsp70 Promoter ◽  
Promoter Sequences ◽  
Highly Active ◽  
Sequence Elements ◽  
Five Elements

Expression from the Drosophila melanogaster hsp70 promoter was controlled by a regulatory unit that was composed of two sequence elements that resembled the heat shock consensus sequence. The unit functioned in both orientations and at different distances from downstream promoter sequences. Each element of the unit alone was essentially inactive. Association of two elements resulted in a dramatic increase of transcription from the hsp70 promoter. This synergistic effect was independent of the relative orientation of the elements and, to a large extent, of the distance between them. Duplication of a region containing only one element also yielded a highly active, heat-regulated promoter. Genes with three to five elements were three to four times more active than those with a single regulatory unit.

scholarly journals B-Myb and cyclin D1 mediate heat shock element dependent activation of the human HSP70 promoter

Oncogene ◽  
1997 ◽  
Vol 14 (10) ◽  
pp. 1223-1229 ◽  
Author(s):  
Hiroshi Kamano ◽  
Karl-Heinz Klempnauer
Keyword(s):  
Heat Shock ◽  
Cyclin D1 ◽  
Hsp70 Promoter ◽  
Heat Shock Element ◽  
Human Hsp70

scholarly journals Organization of the Drosophila melanogaster hsp70 heat shock regulation unit.

1987 ◽  
Vol 7 (3) ◽  
pp. 1055-1062 ◽  
Author(s):  
J Amin ◽  
R Mestril ◽  
P Schiller ◽  
M Dreano ◽  
R Voellmy
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Synergistic Effect ◽  
Consensus Sequence ◽  
Relative Orientation ◽  
Hsp70 Promoter ◽  
Promoter Sequences ◽  
Highly Active ◽  
Sequence Elements ◽  
Five Elements

Expression from the Drosophila melanogaster hsp70 promoter was controlled by a regulatory unit that was composed of two sequence elements that resembled the heat shock consensus sequence. The unit functioned in both orientations and at different distances from downstream promoter sequences. Each element of the unit alone was essentially inactive. Association of two elements resulted in a dramatic increase of transcription from the hsp70 promoter. This synergistic effect was independent of the relative orientation of the elements and, to a large extent, of the distance between them. Duplication of a region containing only one element also yielded a highly active, heat-regulated promoter. Genes with three to five elements were three to four times more active than those with a single regulatory unit.

scholarly journals Definition of essential sequences and functional equivalence of elements downstream of the adenovirus E2A and the early simian virus 40 polyadenylation sites.

1985 ◽  
Vol 5 (11) ◽  
pp. 2975-2983 ◽  
Author(s):  
R P Hart ◽  
M A McDevitt ◽  
H Ali ◽  
J R Nevins
Keyword(s):  
Essential Element ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Cleavage Sites ◽  
Multiple Sequence ◽  
Polyadenylic Acid ◽  
Sequence Elements ◽  
Cleavage And Polyadenylation ◽  
Definition Of ◽  
A Site

In addition to the highly conserved AATAAA sequence, there is a requirement for specific sequences downstream of polyadenylic acid [poly(A)] cleavage sites to generate correct mRNA 3' termini. Previous experiments demonstrated that 35 nucleotides downstream of the E2A poly(A) site were sufficient but 20 nucleotides were not. The construction and assay of bidirectional deletion mutants in the adenovirus E2A poly(A) site indicates that there may be redundant multiple sequence elements that affect poly(A) site usage. Sequences between the poly(A) site and 31 nucleotides downstream were not essential for efficient cleavage. Further deletion downstream (3' to +31) abolished efficient cleavage in certain constructions but not all. Between +20 and +38 the sequence T(A/G)TTTTT was duplicated. Function was retained when one copy of the sequence was present, suggesting that this sequence represents an essential element. There may also be additional sequences distal to +43 that can function. To establish common features of poly(A) sites, we also analyzed the early simian virus 40 (SV40) poly(A) site for essential sequences. An SV40 poly(A) site deletion that retained 18 nucleotides downstream of the cleavage site was fully functional while one that retained 5 nucleotides downstream was not, thus defining sequences required for cleavage. Comparison of the SV40 sequences with those from E2A did not reveal significant homologies. Nevertheless, normal cleavage and polyadenylation could be restored at the early SV40 poly(A) site by the addition of downstream sequences from the adenovirus E2A poly(A) site to the SV40 +5 mutant. The same sequences that were required in the E2A site for efficient cleavage also restored activity to the SV40 poly(A) site.

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