A-to-I mRNA Editing in a Ferric Siderophore Receptor Improves Competition for Iron in Xanthomonas oryzae pv. oryzicola

Microbiology Spectrum ◽

10.1128/spectrum.01571-21 ◽

2021 ◽

Author(s):

Wenhan Nie ◽

Sai Wang ◽

Jin Huang ◽

Qin Xu ◽

Peihong Wang ◽

...

Keyword(s):

Adenosine Deaminase ◽

Rna Editing ◽

Xanthomonas Oryzae ◽

Mrna Editing ◽

Posttranscriptional Modification

Adenosine-to-inosine (A-to-I) RNA editing, which is catalyzed by the adenosine deaminase RNA-specific family of enzymes, is a frequent posttranscriptional modification in metazoans. Research on A-to-I editing in bacteria is limited, and the importance of this editing is underestimated.

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Mutational analysis of apolipoprotein B mRNA editing enzyme (APOBEC1): structure–function relationships of RNA editing and dimerization

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)32141-6 ◽

1999 ◽

Vol 40 (4) ◽

pp. 623-635 ◽

Cited By ~ 1

Author(s):

Ba-Bie Teng ◽

Scott Ochsner ◽

Qian Zhang ◽

Kizhake V. Soman ◽

Paul P. Lau ◽

...

Keyword(s):

Structure Function ◽

Rna Editing ◽

Apolipoprotein B ◽

Mutational Analysis ◽

Mrna Editing ◽

Editing Enzyme

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Recognition of RNA Editing Sites Is Directed by Unique Proteins in Chloroplasts: Biochemical Identification of cis-Acting Elements and trans-Acting Factors Involved in RNA Editing in Tobacco and Pea Chloroplasts

Molecular and Cellular Biology ◽

10.1128/mcb.22.19.6726-6734.2002 ◽

2002 ◽

Vol 22 (19) ◽

pp. 6726-6734 ◽

Cited By ~ 71

Author(s):

Tetsuya Miyamoto ◽

Junichi Obokata ◽

Masahiro Sugiura

Keyword(s):

Rna Editing ◽

Editing Site ◽

Mrna Editing ◽

Higher Plant ◽

In Vitro System ◽

Cis Acting ◽

Biochemical Identification ◽

Editing Activity

ABSTRACT RNA editing in higher-plant chloroplasts involves C-to-U conversions at specific sites. Although in vivo analyses have been performed, little is known about the biochemical aspects of chloroplast editing reactions. Here we improved our original in vitro system and devised a procedure for preparing active chloroplast extracts not only from tobacco plants but also from pea plants. Using our tobacco in vitro system, cis-acting elements were defined for psbE and petB mRNAs. Distinct proteins were found to bind specifically to each cis-element, a 56-kDa protein to the psbE site and a 70-kDa species to the petB site. Pea chloroplasts lack the corresponding editing site in psbE since T is already present in the DNA. Parallel in vitro analyses with tobacco and pea extracts revealed that the pea plant has no editing activity for psbE mRNAs and lacks the 56-kDa protein, whereas petB mRNAs are edited and the 70-kDa protein is also present. Therefore, coevolution of an editing site and its cognate trans-factor was demonstrated biochemically in psbE mRNA editing between tobacco and pea plants.

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dADAR, a Drosophila double-stranded RNA-specific adenosine deaminase is highly developmentally regulated and is itself a target for RNA editing

RNA ◽

10.1017/s1355838200000248 ◽

2000 ◽

Vol 6 (7) ◽

pp. 1004-1018 ◽

Cited By ~ 122

Author(s):

MICHAEL J. PALLADINO ◽

LIAM P. KEEGAN ◽

MARY A. O'CONNELL ◽

ROBERT A. REENAN

Keyword(s):

Adenosine Deaminase ◽

Rna Editing ◽

Developmentally Regulated ◽

Double Stranded Rna

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Intrinsic and regulated properties of minimally edited trypanosome mRNAs

Nucleic Acids Research ◽

10.1093/nar/gkz012 ◽

2019 ◽

Vol 47 (7) ◽

pp. 3640-3657 ◽

Cited By ~ 7

Author(s):

Brianna L Tylec ◽

Rachel M Simpson ◽

Laura E Kirby ◽

Runpu Chen ◽

Yijun Sun ◽

...

Keyword(s):

Rna Editing ◽

High Throughput ◽

High Throughput Sequencing ◽

Substrate Binding ◽

Open Reading Frames ◽

Mrna Editing ◽

Accessory Factors ◽

Reading Frames ◽

Complex Components

Abstract Most mitochondrial mRNAs in kinetoplastids require extensive uridine insertion/deletion editing to generate translatable open reading frames. Editing is specified by trans-acting gRNAs and involves a complex machinery including basal and accessory factors. Here, we utilize high-throughput sequencing to analyze editing progression in two minimally edited mRNAs that provide a simplified system due their requiring only two gRNAs each for complete editing. We show that CYb and MURF2 mRNAs exhibit barriers to editing progression that differ from those previously identified for pan-edited mRNAs, primarily at initial gRNA usage and gRNA exchange. We demonstrate that mis-edited junctions arise through multiple pathways including mis-alignment of cognate gRNA, incorrect and sometimes promiscuous gRNA utilization and inefficient gRNA anchoring. We then examined the roles of accessory factors RBP16 and MRP1/2 in maintaining edited CYb and MURF2 populations. RBP16 is essential for initiation of CYb and MURF2 editing, as well as MURF2 editing progression. In contrast, MRP1/2 stabilizes both edited mRNA populations, while further promoting progression of MURF2 mRNA editing. We also analyzed the effects of RNA Editing Substrate Binding Complex components, TbRGG2 and GAP1, and show that both proteins modestly impact progression of editing on minimally edited mRNAs, suggesting a novel function for GAP1.

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