Performance and Impact on Initial Antibiotic Choice of Direct Identification of Pathogens from Pediatric Blood Culture Bottles Using an In-House MALDI-TOF MS Protocol

Microbiology Spectrum ◽

10.1128/spectrum.01905-21 ◽

2021 ◽

Vol 9 (3) ◽

Author(s):

Andrés Pérez-López ◽

Nazik Elamin ◽

Rhanty Nabor ◽

Sarah Dumindin ◽

Diane Roscoe ◽

...

Keyword(s):

Blood Culture ◽

Preparation Method ◽

Bloodstream Infections ◽

Laser Desorption ◽

Laser Desorption Ionization ◽

Direct Identification ◽

Ionization Time ◽

Flight Mass Spectrometry ◽

Antimicrobial Management ◽

Matrix Assisted Laser

We demonstrate the performance and early impact on the antimicrobial management of bloodstream infections of an inexpensive, in-house preparation method for direct identification of bloodstream pathogens in pediatric blood culture bottles by matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry.

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The sensitivity of direct identification from positive BacT/ALERT™ (bioMérieux) blood culture bottles by matrix-assisted laser desorption ionization time-of-flight mass spectrometry is low

Clinical Microbiology and Infection ◽

10.1111/j.1469-0691.2010.03229.x ◽

2011 ◽

Vol 17 (2) ◽

pp. 192-195 ◽

Author(s):

F. Szabados ◽

M. Michels ◽

M. Kaase ◽

S. Gatermann

Keyword(s):

Mass Spectrometry ◽

Blood Culture ◽

Laser Desorption ◽

Laser Desorption Ionization ◽

Direct Identification ◽

Ionization Time ◽

Desorption Ionization ◽

Flight Mass Spectrometry ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Clinical Microbiology and Infection ◽

10.1111/j.1469-0691.2010.03257.x ◽

2011 ◽

Vol 17 (4) ◽

pp. 546-551 ◽

Author(s):

L. Ferreira ◽

F. Sánchez-Juanes ◽

I. Porras-Guerra ◽

M.I. García-García ◽

J.E. García-Sánchez ◽

...

Keyword(s):

Mass Spectrometry ◽

Blood Culture ◽

Laser Desorption ◽

Laser Desorption Ionization ◽

Direct Identification ◽

Ionization Time ◽

Desorption Ionization ◽

Flight Mass Spectrometry ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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Early adjustment of empirical antibiotic therapy of bloodstream infections on the basis of direct identification of bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and Gram staining results

Journal of Infection and Chemotherapy ◽

10.1016/j.jiac.2020.04.019 ◽

2020 ◽

Vol 26 (9) ◽

pp. 963-969

Author(s):

Ignacio Torres ◽

Carolina Pinto ◽

Rosa Oltra ◽

Tania Pascual ◽

Nieves Carbonell ◽

...

Keyword(s):

Bloodstream Infections ◽

Laser Desorption ◽

Laser Desorption Ionization ◽

Direct Identification ◽

Ionization Time ◽

Flight Mass Spectrometry ◽

Gram Staining ◽

Identification Of Bacteria ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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Direct identification of pathogens from positive blood cultures using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry

Clinical Microbiology and Infection ◽

10.1111/1469-0691.12455 ◽

2014 ◽

Vol 20 (7) ◽

pp. O421-O427 ◽

Author(s):

B. Rodríguez-Sánchez ◽

C. Sánchez-Carrillo ◽

A. Ruiz ◽

M. Marín ◽

E. Cercenado ◽

...

Keyword(s):

Mass Spectrometry ◽

Laser Desorption ◽

Blood Cultures ◽

Laser Desorption Ionization ◽

Direct Identification ◽

Ionization Time ◽

Desorption Ionization ◽

Flight Mass Spectrometry ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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Direct identification of bacteria causing urinary tract infections by combining matrix-assisted laser desorption ionization-time of flight mass spectrometry with UF-1000i urine flow cytometry

Journal of Microbiological Methods ◽

10.1016/j.mimet.2012.12.016 ◽

2013 ◽

Vol 92 (3) ◽

pp. 231-235 ◽

Author(s):

X.-H. Wang ◽

G. Zhang ◽

Y.-Y. Fan ◽

X. Yang ◽

W.-J. Sui ◽

...

Keyword(s):

Laser Desorption ◽

Laser Desorption Ionization ◽

Direct Identification ◽

Ionization Time ◽

Flight Mass Spectrometry ◽

Identification Of Bacteria ◽

Tract Infections ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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Direct identification from positive blood broth culture by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)

The Brazilian Journal of Infectious Diseases ◽

10.1016/j.bjid.2017.03.007 ◽

2017 ◽

Vol 21 (3) ◽

pp. 339-342 ◽

Author(s):

Maria Goreth Barberino ◽

Marcio de Oliveira Silva ◽

Ana Carolina Palmeiras Arraes ◽

Luís Cláudio Correia ◽

Ana Verena Mendes

Keyword(s):

Laser Desorption ◽

Broth Culture ◽

Laser Desorption Ionization ◽

Direct Identification ◽

Ionization Time ◽

Desorption Ionization ◽

Flight Mass Spectrometry ◽

Tof Ms ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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In-house method for direct bacterial identification in positive blood culture broths using microfiltration, bead beating, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Journal of Microbiological Methods ◽

10.1016/j.mimet.2020.106065 ◽

2021 ◽

Vol 180 ◽

pp. 106065

Author(s):

Young Jin Ko ◽

Joong-Ki Kook ◽

Chang Kyu Lee

Keyword(s):

Mass Spectrometry ◽

Blood Culture ◽

Laser Desorption ◽

Bacterial Identification ◽

Laser Desorption Ionization ◽

Ionization Time ◽

Desorption Ionization ◽

Flight Mass Spectrometry ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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Direct identification of Ruminococcus gnavus by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) on a positive anaerobic blood culture bottle

Anaerobe ◽

10.1016/j.anaerobe.2018.07.009 ◽

2018 ◽

Vol 54 ◽

pp. 264-266 ◽

Author(s):

Dionisia Fontanals ◽

Alex Larruzea ◽

Isabel Sanfeliu

Keyword(s):

Laser Desorption ◽

Blood Culture Bottle ◽

Laser Desorption Ionization ◽

Direct Identification ◽

Culture Bottle ◽

Ionization Time ◽

Flight Mass Spectrometry ◽

Tof Ms ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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Performance of Two Resin-Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems

Journal of Clinical Microbiology ◽

10.1128/jcm.01171-14 ◽

2014 ◽

Vol 52 (10) ◽

pp. 3558-3567 ◽

Author(s):

B. Fiori ◽

T. D'Inzeo ◽

V. Di Florio ◽

F. De Maio ◽

G. De Angelis ◽

...

Keyword(s):

Culture Media ◽

Bloodstream Infections ◽

Laser Desorption ◽

Laser Desorption Ionization ◽

Ionization Time ◽

Flight Mass Spectrometry ◽

Isolate Identification ◽

Tof Ms ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) directly from positive blood culture flasks allows rapid identification of bloodstream infections in immunosuppressed hosts

Transplant Infectious Disease ◽

10.1111/tid.12373 ◽

2015 ◽

Vol 17 (3) ◽

pp. 481-487 ◽

Author(s):

A. Egli ◽

M. Osthoff ◽

D. Goldenberger ◽

J. Halter ◽

S. Schaub ◽

...

Keyword(s):

Mass Spectrometry ◽

Bloodstream Infections ◽

Laser Desorption ◽

Rapid Identification ◽

Laser Desorption Ionization ◽

Ionization Time ◽

Desorption Ionization ◽

Flight Mass Spectrometry ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

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