Isolation and crystallization of heterotrimeric translation initiation factor 2 from Sulfolobus solfataricus

2009 ◽  
Vol 74 (1) ◽  
pp. 54-60 ◽  
Author(s):  
E. A. Stolboushkina ◽  
O. S. Nikonov ◽  
M. B. Garber
Keyword(s):  
Translation Initiation ◽  
Sulfolobus Solfataricus ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Initiation Factor 2 ◽  
Translation Initiation Factor 2

Conformational transitions in the γ subunit of the archaeal translation initiation factor 2

2014 ◽  
Vol 70 (3) ◽  
pp. 658-667 ◽  
Author(s):  
Oleg Nikonov ◽  
Elena Stolboushkina ◽  
Valentina Arkhipova ◽  
Olesya Kravchenko ◽  
Stanislav Nikonov ◽  
...  
Keyword(s):  
Translation Initiation ◽  
Inorganic Phosphate ◽  
Bound States ◽  
Sulfolobus Solfataricus ◽  
Conformational Transitions ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Γ Subunit ◽  
Initiation Factor 2 ◽  
Translation Initiation Factor 2

In eukaryotes and archaea, the heterotrimeric translation initiation factor 2 (e/aIF2) is pivotal for the delivery of methionylated initiator tRNA (Met-tRNAi) to the ribosome. It acts as a molecular switch that cycles between inactive (GDP-bound) and active (GTP-bound) states. Recent studies show that eIF2 can also exist in a long-lived eIF2γ–GDP–Pi(inorganic phosphate) active state. Here, four high-resolution crystal structures of aIF2γ fromSulfolobus solfataricusare reported: aIF2γ–GDPCP (a nonhydrolyzable GTP analogue), aIF2γ–GDP–formate (in which a formate ion possibly mimics Pi), aIF2γ–GDP and nucleotide-free aIF2γ. The structures describe the different states of aIF2γ and demonstrate the conformational transitions that take place in the aIF2γ `life cycle'.

Binding of the 5′-Triphosphate End of mRNA to the γ-Subunit of Translation Initiation Factor 2 of the Crenarchaeon Sulfolobus solfataricus

2015 ◽  
Vol 427 (19) ◽  
pp. 3086-3095 ◽  
Author(s):  
Valentina Arkhipova ◽  
Elena Stolboushkina ◽  
Olesya Kravchenko ◽  
Vladislav Kljashtorny ◽  
Azat Gabdulkhakov ◽  
...  
Keyword(s):  
Translation Initiation ◽  
Sulfolobus Solfataricus ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Γ Subunit ◽  
Initiation Factor 2 ◽  
Translation Initiation Factor 2
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