Genetic diversity across natural populations of Dendrobium officinale, the endangered medicinal herb endemic to China, revealed by ISSR and RAPD markers

The results of spatial analysis of genetic diversity have practical application in the definition and delineation of regional provenances of forest trees. Research in this paper, was based on the material from 27 natural populations of beech in Serbia. The genetic component of the research is based on the analysis of RAPD markers from bulk samples, using 28 primers. The spatial component of the research is based on the geographical position of the studied populations. Grouping of the studied populations in the regions, as well as their separation, was performed using the Monmonier?s algorithm of maximum differences. To visualize the results and mapping the regions of beech provenances in Serbia, GIS was used, with database included the results of this study.

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Genetic diversity in natural populations of the medicinal herb Polygala tenuifolia Willd. and its implications for conservation

Biochemical Systematics and Ecology ◽

10.1016/j.bse.2012.06.016 ◽

2012 ◽

Vol 44 ◽

pp. 400-406 ◽

Cited By ~ 5

Author(s):

Min-Feng Fang ◽

Jia Li ◽

Tian-Hua Zhou ◽

Jia Yang ◽

Gui-Fang Zhao

Keyword(s):

Genetic Diversity ◽

Natural Populations ◽

Medicinal Herb ◽

Polygala Tenuifolia

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Genetic polymorphism among natural populations of Anacardium humile A. ST-HIL

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452020476 ◽

2020 ◽

Vol 42 (1) ◽

Cited By ~ 1

Author(s):

Silvia Correa Santos ◽

Raquel dos Santos Carvalho ◽

Livia Maria Chamma Davide

Keyword(s):

Genetic Diversity ◽

Genetic Polymorphism ◽

Genetic Variability ◽

Genetic Divergence ◽

Genomic Dna ◽

Reproductive Strategies ◽

Rapd Markers ◽

Phenotypic Diversity ◽

Diversity Index ◽

Natural Populations

Abstract The genus Anacardium presents nine species, of these, three have sub-bush size, common in the Cerrado of the Center-West of Brazil. The objective of this work was to evaluate the genetic variability of the species, collected in eleven provenances, using RAPD markers. Genomic DNA from 122 accessions was extracted and amplified with 25 decamer primers. The results indicated polymorphism, ranging from 77.71% to 96.18%. The distribution of genetic diversity among and within populations shows that 27.14% of the variability is found between populations and 37.44% within the populations, suggesting the existence of genetic variability that may be related to the reproductive strategies adopted by the species throughout its evolution. The index of variation within the provenances (93.36%) was higher than the index found among populations (6.64%). Molecular analysis indicated that there is genetic divergence between and within the studied populations of Anacardium humile A. St. - Hill. The origin of Itajá-GO presented the highest genetic diversity, presenting the highest values of genetic diversity index, phenotypic diversity and higher percentage of polymorphic loci.

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Assessment of Genetic Diversity in Five Nicaraguan Populations of Cedrela odorata L. (Meliaceae) using RAPD Markers

Encuentro ◽

10.5377/encuentro.v0i103.2690 ◽

2016 ◽

pp. 28-39

Author(s):

Arlen Tijerino ◽

Lourdes Callejas ◽

David A. Cerda-Granados

Keyword(s):

Genetic Diversity ◽

Genetic Differentiation ◽

Rapd Markers ◽

Diversity Index ◽

Random Amplified Polymorphic Dna ◽

Natural Populations ◽

Cedrela Odorata ◽

Total Genetic Diversity ◽

Geographical Distances ◽

The Mean

The goal of this study was to assess the genetic diversity of Nicaraguan populations of Cedrela odorata using Random Amplified Polymorphic DNA (RAPD) markers. Thus, genomic DNA was isolated from leaf samples collected from ninety-two trees belonging to five Nicaraguan natural populations of C. odorata. The mean number of alleles per locus, effective number of alleles per locus, percentage of polymorphic loci, genetic diversity (He ) of Nei and diversity index (Ho ) of Shannon were estimated for each population assuming that the populations were in HardyWeinberg equilibrium. Total genetic diversity was partitioned in intrapopulational and interpopulational diversity using Nei’s genetic differentiation (GST) and through an Analysis of Molecular Variance (AMOVA). The ΦST matrix was used to construct a dendrogram by the neighbor-joining method. According to values of both He and Ho , Esquipulas (Deparment of Matagalpa) presented the lowest diversity level; while La Trinidad (Department of Estelí) showed the highest diversity level. Genetic differentiation was calculated obtaining a GST value of 13.36%. AMOVA also showed a similar differentiation value ΦST =13.81%). Neighbour-joining dendrogram clustered the five populations in two groups, where the group formed by La Trinidad and El Refugio (Department of Granada) presented the biggest differentiation. Correlation between genetic and geographical distances was not found.

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Analysis of genetic diversity through AFLP, SAMPL, ISSR and RAPD markers in Tribulus terrestris, a medicinal herb

Plant Cell Reports ◽

10.1007/s00299-007-0478-5 ◽

2007 ◽

Vol 27 (3) ◽

pp. 519-528 ◽

Cited By ~ 57

Author(s):

Maryam Sarwat ◽

S. Das ◽

P. S. Srivastava

Keyword(s):

Genetic Diversity ◽

Rapd Markers ◽

Medicinal Herb ◽

Tribulus Terrestris

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Assessment of Genetic Diversity, Population Structure and Phytochemical Variations in Polygonatum Cirrhifolium (Wall.) Royle - An Endangered Medicinal Herb

10.21203/rs.3.rs-1110766/v1 ◽

2021 ◽

Author(s):

Renu Suyal ◽

Arun K. Jugran ◽

Indra D. Bhatt ◽

Ranbeer S. Rawal

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Genetic Erosion ◽

Management Plan ◽

Issr Markers ◽

Medicinal Herb ◽

Antimutagenic Activity ◽

Dna Recovery ◽

Population Structure Analysis ◽

Endangered Medicinal Herb

Abstract Polygonatum cirrhifolium is an important medicinal herb of family Asparagaceae used to cure several ailments. Its rhizome forms an important ingredient of “Chyavanprash” which is identified for its rejuvenating properties. However, P.cirrhifolium is least explored scientifically and systematically till date. Therefore, in this study genetic diversity and phytochemical variations along with antimutagenic activity of P.cirrhifolium populations was evaluated. Antimutagenic activity varied remarkably (p<0.05) having Gagar population with significantly (p<0.05) higher DNA recovery (84.95%) percentage. Higher genetic diversity (He) was recorded among populations using RAPD (He, 0.30-0.36) and ISSR (0.25-0.38) markers. High intra and low inter population variations were recorded in the species using both kinds of markers. Phenolics (p<0.05; r=0.924); tannins (p<0.05; r=0.897) and DNA damage inhibition efficiency displayed a highly positive correlation with genetic diversity (estimated using ISSR markers). The population structure analysis of P. cirrhifolium revealed that the greatest value of the K was 3 for studied populations. Gene flow among studied populations was found sufficient to encounter genetic erosion in the species. Therefore, it can be recommended that the populations with higher ingredient and genetic diversity can be utilized for conservation priority and management plan of this species.

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