Protein spectrum of the erythrocyte membrane and its changes in pathology

Author(s):  
I. V. Babushkin ◽  
Yu. I. Pivovarov ◽  
T. E. Kurilskaya ◽  
A. S. Sergeeva ◽  
O. P. Iljina ◽  
...  
Author(s):  
Neng-Bo He ◽  
S.W. Hui

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).


Diabetes ◽  
1989 ◽  
Vol 38 (12) ◽  
pp. 1539-1543 ◽  
Author(s):  
S. K. Jain ◽  
R. McVie ◽  
J. Duett ◽  
J. J. Herbst

Diabetes ◽  
1987 ◽  
Vol 36 (9) ◽  
pp. 991-995 ◽  
Author(s):  
D. Rahmani-Jourdheuil ◽  
Y. Mourayre ◽  
P. Vague ◽  
J. Boyer ◽  
I. Juhan-Vague

BIOPHYSICS ◽  
2020 ◽  
Vol 65 (4) ◽  
pp. 614-618
Author(s):  
I. V. Petrova ◽  
Yu. G. Birulina ◽  
S. N. Belyaeva ◽  
O. A. Trubacheva ◽  
A. V. Sidekhmenova ◽  
...  

Blood ◽  
1983 ◽  
Vol 61 (2) ◽  
pp. 373-377 ◽  
Author(s):  
JE Smith ◽  
K Moore ◽  
M Arens ◽  
GA Rinderknecht ◽  
A Ledet

Abstract A dog with persistent elliptocytosis was studied. The dog had membrane protein band 4.1 deficiency, microcytosis, shortened erythrocyte lifespan, increased osmotic sensitivity, and a mild glutathione deficiency. Erythrocyte deformability and membrane stability were adversely effected. The dog's parents had decreased band 4.1, decreased stability, and some elliptocytosis. This disorder in dogs closely resembles human patients with band 4.1 deficiency and should provide a valuable animal model to study the erythrocyte membrane cytoskeleton.


Vox Sanguinis ◽  
1974 ◽  
Vol 26 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Michael J. Conrad ◽  
John T. Penniston

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