Morphology of Nicotiana tabacum cells grown in contact with lunar material

Suspension and stationary habituated tobacco tissue cultures were examined histologically and cytologically after treatment with 0.22 g of lunar material. The treated and untreated tissues differed in chloroplast structure and distribution, degree of cell association, cytoplasmic vacuolation and vesiculation, and living to nonliving ratios.

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Apollo 12 Lunar Material: Effects on Lipid Levels of Tobacco Tissue Cultures

Science ◽

10.1126/science.175.4022.623 ◽

1972 ◽

Vol 175 (4022) ◽

pp. 623-624 ◽

Cited By ~ 3

Author(s):

J. D. Weete ◽

C. H. Walkinshaw ◽

J. L. Laseter

Keyword(s):

Tissue Cultures ◽

Lipid Levels ◽

Lunar Material

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Cytokinin autotrophy and differentiation in tissue cultures of haploid Nicotiana tabacum L.

Plant Science ◽

10.1016/0168-9452(86)90048-8 ◽

1986 ◽

Vol 45 (2) ◽

pp. 125-132 ◽

Cited By ~ 7

Author(s):

Gilberto Barbante Kerbauy ◽

JoséAntonio Peters ◽

Kurt Gunther Hell

Keyword(s):

Nicotiana Tabacum ◽

Tissue Cultures ◽

Nicotiana Tabacum L

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Growth and Division of Single Cells of Higher Plants in Vitro

The Journal of General Physiology ◽

10.1085/jgp.43.4.841 ◽

1960 ◽

Vol 43 (4) ◽

pp. 841-851 ◽

Cited By ~ 169

Author(s):

Ludwig Bergmann

Keyword(s):

Nicotiana Tabacum ◽

Higher Plants ◽

Single Cells ◽

Tissue Cultures ◽

Phaseolus Vulgaris L ◽

Isolated Cells ◽

Nicotiana Tabacum L ◽

Agar Layer ◽

Petri Dishes

The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope.

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Schnellmarkierte RNS in heterotroph kultivierten Geweben von Nicotiana tabacum / Rapidly Labelled RNA in Heterotrophically Cultured Tissues of Nicotiana tabacum

Zeitschrift für Naturforschung B ◽

10.1515/znb-1972-1021 ◽

1972 ◽

Vol 27 (10) ◽

pp. 1205-1215 ◽

Cited By ~ 9

Author(s):

Wolfgang Beisenherz

Keyword(s):

Nicotiana Tabacum ◽

Thin Layer ◽

Chromatographic Separation ◽

Sedimentation Coefficient ◽

Life Time ◽

Rna Metabolism ◽

Tissue Cultures ◽

Differential Centrifugation ◽

Sedimentation Analysis ◽

Tissue Homogenates

The RNA metabolism in tissue cultures of Nicotiana tabacum has been studied by incorporation of radioactive phosphate. The RNA was extracted by phenol-kresol-hydroxychinon and fractionated by MAK columns. A rapidly labelled RNA fraction was eluted from the MAK column after the rRNA components. Its base ratio differed from the latter in that the AMP content (28,6%) was higher than the GMP content (26,0%). Sedimentation analysis on sucrose gradients showed a sedimentation coefficient of 37 -38s. The life time of the 37-38s RNA fraction in chase experiments was 3,5-4 hours. Differential centrifugation of tissue homogenates revealed that the labelled 37-38s RNA was associated with particles sedimented by low speed centrifugation. No 37-38s RNA could be detected in the ribosomal fraction and the supernatant. During chase experiments label appeared in the rRNA components. It could be excluded by thin layer chromatographic separation of 32P labelled compounds that this label originated from 32P-o-phosphate or labelled organic phosphates except of the rapidly labelled 37-38s RNA. From this it was concluded that the 37-38s RNA is a precursor of the rRNA.

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Growth Inhibition in Tobacco (Nicotiana tabacum) Callus by 2,6-Dinitroaniline Herbicides and Protection by D-α-tocopherol Acetate

Weed Science ◽

10.1017/s0043174500064468 ◽

1978 ◽

Vol 26 (6) ◽

pp. 527-530 ◽

Cited By ~ 12

Author(s):

J. B. Huffman ◽

N. D. Camper

Keyword(s):

Regression Analysis ◽

Nicotiana Tabacum ◽

Callus Tissue ◽

Linear Regression Analysis ◽

Tissue Growth ◽

Tissue Cultures ◽

Fresh Weight ◽

Structural Variations ◽

Tocopherol Acetate ◽

Dinitroaniline Herbicides

Tobacco(Nicotiana tabacumL. ‘X-73’) callus tissue cultures were used in a bioassay system for determining the effect of the following substituted 2,6-dinitroaniline herbicides on growth: trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine); oryzalin (3,5-dinitro-N4,N4-dipropylsulfanilamide); benefin(N-butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-toluidine); AC 92390(N-sec-butyl-2,6-dinitro-3,4-xylidine); penoxalin [N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine]; GS-38946(N-ethyl-N-tetrahydrofurfuryl-4-trifluoromethyl-2,6-dinitroaniline); chlornidine [N,N-di(2-chloroethyl)-4-methyl-2,6-dinitroaniline]; nitralin [4-(methylsulfonyl)2,6-dinitro-N,N-dipropylaniline]; dinitramine(N4,N4-diethyl-α,α,α-trifluoro-3,5-dinitrotoluene-2,4-diamine); isopropalin (2,6-dinitro-N,N-dipropylcumidine), and profluralin [N(cyclopropylmethyl)-α,α,α-trifluoro-2,6-dinitro-N-propyl-p-toluidine]. The molar concentration required to inhibit fresh weight gain by 50% (I50) was determined by using linear regression analysis on data from a range of five concentrations of each chemical. I50values did not correlate with structural variations or available physical constants. Herbicides listed in order of increasing activity are AC 92390< GD-38946<profluralin = isopropalin<benefin = chlornidine = trifluralin = nitralin<oryzalin = penoxalin < dinitramine. Exogenously applied D-α-tocopherol acetate at 100 times the I50concentrations decreased the inhibition of tissue growth by the herbicides.

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