Growth Inhibition in Tobacco (Nicotiana tabacum) Callus by 2,6-Dinitroaniline Herbicides and Protection by D-α-tocopherol Acetate

Weed Science ◽  
1978 ◽  
Vol 26 (6) ◽  
pp. 527-530 ◽  
Author(s):  
J. B. Huffman ◽  
N. D. Camper

Tobacco(Nicotiana tabacumL. ‘X-73’) callus tissue cultures were used in a bioassay system for determining the effect of the following substituted 2,6-dinitroaniline herbicides on growth: trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine); oryzalin (3,5-dinitro-N4,N4-dipropylsulfanilamide); benefin(N-butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-toluidine); AC 92390(N-sec-butyl-2,6-dinitro-3,4-xylidine); penoxalin [N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine]; GS-38946(N-ethyl-N-tetrahydrofurfuryl-4-trifluoromethyl-2,6-dinitroaniline); chlornidine [N,N-di(2-chloroethyl)-4-methyl-2,6-dinitroaniline]; nitralin [4-(methylsulfonyl)2,6-dinitro-N,N-dipropylaniline]; dinitramine(N4,N4-diethyl-α,α,α-trifluoro-3,5-dinitrotoluene-2,4-diamine); isopropalin (2,6-dinitro-N,N-dipropylcumidine), and profluralin [N(cyclopropylmethyl)-α,α,α-trifluoro-2,6-dinitro-N-propyl-p-toluidine]. The molar concentration required to inhibit fresh weight gain by 50% (I50) was determined by using linear regression analysis on data from a range of five concentrations of each chemical. I50values did not correlate with structural variations or available physical constants. Herbicides listed in order of increasing activity are AC 92390< GD-38946<profluralin = isopropalin<benefin = chlornidine = trifluralin = nitralin<oryzalin = penoxalin < dinitramine. Exogenously applied D-α-tocopherol acetate at 100 times the I50concentrations decreased the inhibition of tissue growth by the herbicides.

2014 ◽  
Vol 64 (4) ◽  
pp. 366-370
Author(s):  
E. Szczepkowska-Kuźnicka ◽  
W. Wardas

The influence of benzene, phenol and catechol on the tobacco callus tissue (<i>Nicotiana tabacum</i> L. cv. Wisconsin No. 38) cultivated <i>in vitro</i> was studied. Although inhibition of callus tissue growth in relation to the control was observed in all variants, the benzene-induced inhibition was the most significant. When catechol and phenol were added to the cultivation medium together, callus tissue growth was similar to that of the control. The addition of catechol to the medium containing phenol presumably reduces the toxic effect of phenol on the callus tissue examined. Callus tissue growing in the presence of phenol, benzene and cathechol became senescent more rapidly than in the control. Moreover microscopic examination revealed significant changes in the callus structure.


1988 ◽  
Vol 43 (9-10) ◽  
pp. 636-640 ◽  
Author(s):  
Andreas B. Wolff ◽  
Maung Thynn ◽  
Elisabeth Gorge ◽  
Dietrich Werner

Abstract Callus cultures of various cultivars of Vicia faba were studied for regeneration. During callus growth on agar or in liquid medium a black tissue was often observed correlated with an autolysis of some cells. With those tissues callus growth and regeneration of plants was inhibited. In black callus the phytoalexin synthesis is induced without infection by microorganism. The main phytoalexins in the callus tissue are wyerol and wyerone, with some wyeronic acid and dihydrowyerone. Wyerol was found especially in the cultivar Troy at a high concentration. In four cultivars the pooled phytoalexin concentration varied between 10 and 150 μg · g-1 fresh weight.


2015 ◽  
Vol 43 (1) ◽  
pp. 59-70
Author(s):  
Z. Chirek

A significant limitation of callus tissue growth was noted in <i>Nicotiana tabacum</i> L. cultured in vitro under the influence of morphactin IT 3233 applied in 10 - 40 mg/dm<sup>3</sup> concentrations. Growth inhibition was associated with an increase in dry mass content and the contribution of protein N to the nitrogen, pool. Tumour tissue of tobacco under the same conditions reacted weaker to morphactin. Growth limitation reached 25 per cent as compared with the control, dry mass and nitrogen compounds content showed only slight variations. It is supposed that the different sensitivity of both types of tissues to morphactin is connected with their different hormonal metabolism.


2015 ◽  
Vol 43 (1) ◽  
pp. 71-80
Author(s):  
Z. Chirek

It was found that the inhibition of callus tissue growth in <i>Nicotiana tabacum</i> L. cultured <i>in vitro</i> by the application of morphactin IT 3233 is associated with a rise of the protein level in spite of a 50 per cent depression of its synthesis. Respiratory activity of these tissues is also lowered. Tumour tissues, on the other hand, show only light changes in the parameters studied. It would seem that morphactin depresses a metabolic activity in the callus tissues, and probably causes deposition of a, large quantity of metabolically inactive proteins.


2003 ◽  
Vol 1 (1) ◽  
pp. 91-102 ◽  
Author(s):  
Steve E. Shive ◽  
Michelle R. Neyman

This study examined fruit intake among 276 female Extended Opportunity Program and Services (EOPS) community college students. Knowledge, attitudes, and behaviors associated with fruit intake were determined. The attitudes which are potential predictors of daily fruit intake were also examined. Analysis showed that 33% ate fewer than the minimum recommended two daily servings of fruit. Students reported that fruit gave them energy, taste and cost were not barriers to consumption and that it would not be difficult to increase daily fruit consumption. A linear regression analysis revealed that beliefs that eating fruit leads to good health, having a habit of eating fruit, feeling energy, and weight loss accounted for 30% of the variance in fruit intake.


Author(s):  
Muhammad Rois Rois ◽  
Manarotul Fatati Fatati ◽  
Winda Ihda Magfiroh

This study aims to determine the effect of Inflation, Exchange Rate and Composite Stock Price Index (IHSG) to Return of PT Nikko Securities Indonesia Stock Fund period 2014-2017. The study used secondary data obtained through documentation in the form of PT Nikko Securities Indonesia Monthly Net Asset (NAB) report. Data analysis is used with quantitative analysis, multiple linear regression analysis using eviews 9. Population and sample in this research are PT Nikko Securities Indonesia. The result of multiple linear regression analysis was the coefficient of determination (R2) showed the result of 0.123819 or 12%. This means that the Inflation, Exchange Rate and Composite Stock Price Index (IHSG) variables can influence the return of PT Nikko Securities Indonesia's equity fund of 12% and 88% is influenced by other variables. Based on the result of the research, the variables of inflation and exchange rate have a negative and significant effect toward the return of PT Nikko Securities Indonesia's equity fund. While the variable of Composite Stock Price Index (IHSG) has a negative but not significant effect toward Return of Equity Fund of PT Nikko Securities Indonesia


Liquidity ◽  
2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Yanti Budiasih

The objectives of this study are to analyze changes in organizational structure, job design, organizational culture and its influence on employee productivity at PT. XX in Jakarta and to identify variables that have a dominant influence on the productivity of employees. The research method used is using multiple linear regression analysis. The results show that the all variables simultaneously and partially change the organizational structure, job design, and organizational culture has a significant impact on employee productivity at PT. XXin Jakarta.


10.32698/0642 ◽  
2019 ◽  
Vol 2 (2) ◽  
pp. 120
Author(s):  
Wiwi Delfita ◽  
Neviyarni S. ◽  
Riska Ahmad

Some students perceive lesbian, gay, bisexual, and transgender (LGBT) positively, even though LGBT is a sexual deviation that is not appropriate with values and norms. There are several factors that influence an individual's perception of LGBT, including sexual identity. This study aims at looking at the contribution of sexual identity to student perceptions about LGBT. This research used a quantitative approach with a descriptive method and a simple linear regression analysis. The sample of this research was 385 taken from 15.752 undergraduate students of Universitas Negeri Padang which the sample was drawn by using the Slovin formula and continued with a Proportional Random Sampling technique. The instrument used was the Guttman model's sexual identity scale and the scale of students' perceptions of the LGBT Likert model. After analyzing the data with the descriptive technique and the simple linear regression analysis, the results showed that sexual identity significantly contributed to the students' perceptions of LGBT. This research has implications as a basis for counselors to help students avoid sexual identity mismatches and prevent the emergence of positive perceptions of LGBT.


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