Phylogeny of the Erysiphales (powdery mildews) inferred from internal transcribed spacer ribosomal DNA sequences

1999 ◽  
Vol 77 (1) ◽  
pp. 150-168 ◽  
Author(s):  
Gregory S. Saenz ◽  
John W. Taylor
Keyword(s):  
Ribosomal Dna ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Powdery Mildews

Phylogenetic analysis of Phylica L. ( Rhamnaceae ) with an emphasis on island species: evidence from plastid trnL‐F and nuclear internal transcribed spacer (ribosomal) DNA sequences

Taxon ◽  
2001 ◽  
Vol 50 (2) ◽  
pp. 405-427 ◽  
Author(s):  
James E. Richardson ◽  
Frans M. Weitz ◽  
Michael F. Fay ◽  
Quentin C. B. Cronk ◽  
H. Peter Linder ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Island Species

Characterization of the complete nuclear ribosomal DNA sequences of Eurytrema pancreaticum

2017 ◽  
Vol 92 (4) ◽  
pp. 484-490 ◽  
Author(s):  
X. Su ◽  
Y. Zhang ◽  
X. Zheng ◽  
X.X. Wang ◽  
Y. Li ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Dna Sequences ◽  
18S Rdna ◽  
Internal Transcribed Spacer ◽  
28S Rdna ◽  
Nuclear Ribosomal Dna ◽  
Economic Losses ◽  
Length Variation ◽  
5.8S Rdna ◽  
Rdna Sequences

AbstractEurytrema pancreaticum is one of the most common trematodes of cattle and sheep, and also infects humans occasionally, causing great economic losses and medical costs. In this study, the sequences of the complete nuclear ribosomal DNA (rDNA) repeat units of five E. pancreaticum individuals were determined for the first time. They were 8306–8310 bp in length, including the small subunit (18S) rDNA, internal transcribed spacer 1 (ITS1), 5.8S rDNA, internal transcribed spacer 2 (ITS2), large subunit (28S) rDNA and intergenic spacer (IGS). There were no length variations in any of the investigated 18S (1996 bp), ITS1 (1103 bp), 5.8S (160 bp), ITS2 (231 bp) or 28S (3669 bp) rDNA sequences, whereas the IGS rDNA sequences of E. pancreaticum had a 4-bp length variation, ranging from 1147 to 1151 bp. The intraspecific variations within E. pancreaticum were 0–0.2% for 18S rDNA, 0–0.5% for ITS1, 0% for 5.8S rDNA and ITS2, 0–0.2% for 28S rDNA and 2.9–20.2% for IGS. There were nine types of repeat sequences in ITS1, two types in 28S rDNA, but none in IGS. A phylogenetic analysis based on the 18S rDNA sequences classified E. pancreaticum in the family Dicrocoeliidae of Plagiorchiata, closely related to the suborder Opisthorchiata. These results provide useful information for the further study of Dicrocoeliidae trematodes.

PHYLOGENY OF HEDYCHIUM AND RELATED GENERA (ZINGIBERACEAE) BASED ON ITS SEQUENCE DATA

2000 ◽  
Vol 57 (2) ◽  
pp. 261-270 ◽  
Author(s):  
T. H. WOOD ◽  
W. M. WHITTEN ◽  
N. H. WILLIAMS
Keyword(s):  
Ribosomal Dna ◽  
Dna Sequences ◽  
Interspecific Hybrid ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Cladistic Analysis ◽  
Monophyletic Group ◽  
Nuclear Ribosomal Dna ◽  
Its Sequence ◽  
Limited Sample

The phylogeny of Hedychium J. Koenig was estimated using sequence data of internal transcribed spacer regions 1 and 2 (ITS1, ITS2) and 5.8S nuclear ribosomal DNA. Sequences were determined for 29 taxa, one interspecific hybrid of Hedychium and one species in each of 16 other genera of Zingiberaceae representing tribes Hedychieae, Globbeae, Zingibereae and Alpinieae. Cladistic analysis of these data strongly supports the monophyly of Hedychium, but relationships to other genera are poorly supported. Within Hedychium, four major clades are moderately supported. These clades are also distinguishable on the basis of number of flowers per bract and distribution. Stahlianthus, Curcuma, and Hitchenia also form a strongly supported clade. Based on this limited sample, the currently defined tribes of Zingiberoideae are not monophyletic. The Asiatic genera form a monophyletic group within this broadly defined Hedychieae. The taxonomy and biogeography of Hedychium are reviewed.

Gymnopus barbipes and G. dysodes, new records for Pakistan

Mycotaxon ◽  
2020 ◽  
Vol 135 (1) ◽  
pp. 203-212
Author(s):  
Malka Saba ◽  
Junaid Khan ◽  
Samina Sarwar ◽  
Hassan Sher ◽  
Abdul Nasir Khalid
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
New Records ◽  
Phylogenetic Analyses ◽  
Internal Transcribed Spacer Region

Morphological and phylogenetic analyses were conducted to identify Gymnopus species collected in Pakistan during 2013–14. Phylogenetic analysis was performed using internal transcribed spacer region (ITS) of ribosomal DNA sequences. Among the collected taxa, we identified Gymnopus barbipes and G. dysodes, represent new records for Pakistan. Their detailed descriptions and illustrations are also provided.

Helminth parasites of shags (Phalacrocoracidae) from the Otago region of southern New Zealand

2021 ◽  
Vol 95 ◽  
Author(s):  
B. Presswell ◽  
J. Bennett
Keyword(s):  
New Zealand ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Helminth Parasites ◽  
Phalacrocorax Carbo ◽  
New Host ◽  
Contracaecum Rudolphii ◽  
Its1 And Its2 ◽  
New Host Records

Abstract A total of 61 specimens representing five species of shag – Auckland Island shag Leucocarbo colensoi, little pied shag Microcarbo melanoleucos brevirostris, black shag Phalacrocorax carbo novaehollandiae, Otago shag Leucocarbo chalconotus and spotted shag Phalacrocorax punctatus – from the coast around Otago, South Island, New Zealand, were examined for helminths. A total of 18 helminth species was found: six nematodes (Anisakis pegreffi, Contracaecum rudolphii E, Baruscapillaria sp., Cosmocephalus jaenschi, Ingliseria cirrohamata, Desmidocercella australis), four trematodes (Apatemon sp. ‘jamiesoni’, Cardiocephaloides ovicorpus, Apophallus sp., Microphallidae gen. sp.), four cestodes (Microsomacanthus cormoranti, Microsomacanthus sp., Paradilepis urceina, Tetrabothrius sp.) and four acanthocephalans (Andracantha leucocarboi, A. sigma, Corynosoma hannae, Profilicollis novaezelandensis). Descriptions are provided for females of C. jaenschi and D. australis, which were previously undescribed. The data include 20 new host records and seven new locality records. New 18S ribosomal DNA and internal transcribed spacer (ITS1 and ITS2) DNA sequences have been provided where specimen conditions permitted. These data add considerably to our sparse knowledge of helminths in New Zealand shags, and provide a baseline for observations of change in the future.

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