The genetic basis of seed set in barley genotypes varying in compatibility with Hordeum bulbosum

Genome ◽  
1992 ◽  
Vol 35 (5) ◽  
pp. 799-805 ◽  
Author(s):  
F. Q. Chen ◽  
P. M. Hayes
Keyword(s):  
Doubled Haploid ◽  
Segregation Analysis ◽  
Production Efficiency ◽  
Genetic Basis ◽  
Seed Set ◽  
Hordeum Bulbosum ◽  
Hordeum Vulgare L ◽  
Wide Crosses ◽  
Haploid Production ◽  
Barley Genotypes

Low seed set, owing to partial incompatibility, can limit sexual gene transfer and haploid production efficiency in wide crosses. The inheritance of partial incompatibility in barley Hordeum vulgare L. × H. bulbosum L. crosses and its effect on gamete sampling in doubled haploid production were studied by doubled haploid progeny analysis. The dominant, monogenic control of partial incompatibility in 'Vada' was confirmed. Partial incompatibility in 'Harrington' is also monogenic but appears to be controlled by a different gene. An association between the Inc gene and a deficiency in a stigma–stylodium specific high pI protein was found in the co-segregation analysis of doubled haploid progeny. Segregation analysis of Mendelian markers in doubled haploid progeny showed that there is no evidence that the compatibility status of the parents has an effect on gamete sampling by the bulbosum technique.Key words: barley, haploids, Hordeum bulbosum, incompatibility.

Optimization of chromosome doubling treatment for efficient in vivo doubled haploid production in maize

2021 ◽  
pp. 1-10
Author(s):  
Sourbh Kumar ◽  
Uttam Chandel ◽  
Satish Kumar Guleria
Keyword(s):  
Doubled Haploid ◽  
Seed Set ◽  
Chromosome Doubling ◽  
Pollen Viability ◽  
Haploid Inducer ◽  
Haploid Production ◽  
Maize Genotypes ◽  
Different Doses

Abstract An investigation to optimize the protocol for application of colchicine for enhancing the doubled haploid production in maize was done. 106 maize genotypes were used as maternal parents, whereas, pollen source involved tropically adopted haploid inducer (TAIL P1 and TAIL hybrid). After the elimination of chromosomes of inducer lines, haploid seeds were obtained from the crosses. Haploid seedlings were treated with three different doses, such as 0.04, 0.06 and 0.08 per cent of colchicines for different durations (8, 12 and 15 hours). The response of various colchicine concentrations applied for different time durations revealed significant differences at P ≤ 0.05 for various parameters viz., per cent plants survivability, stalk colour, the fertility of tassel, silk present/absent, pollen viability, seed set and per cent doubled haploid formation. In maize, colchicine doses of 0.04 per cent for 12 hours and 0.06 per cent for 8 hours, respectively were established as optimum for enhanced doubled haploid production. But among these two, 0.04 per cent for 12 hours was observed to be best dose for doubled haploid production in maize.

Improving in vivo maize doubled haploid production efficiency through early detection of false positives

2012 ◽  
Vol 131 (3) ◽  
pp. 399-401 ◽  
Author(s):  
Eunsoo Choe ◽  
Christine Hayot Carbonero ◽  
Kelly Mulvaney ◽  
A. Lane Rayburn ◽  
Rita H. Mumm
Keyword(s):  
Early Detection ◽  
Doubled Haploid ◽  
Production Efficiency ◽  
False Positives ◽  
Haploid Production

Improving androgenesis-mediated doubled haploid production efficiency of FCV tobacco (Nicotiana tabacumL.) throughin vitrocolchicine application

2013 ◽  
Vol 132 (6) ◽  
pp. 764-771 ◽  
Author(s):  
Salej Sood ◽  
Samresh Dwivedi ◽  
Thamalampudi Venkata Reddy ◽  
Panuganti Satya Prasanna ◽  
Navin Sharma
Keyword(s):  
Doubled Haploid ◽  
Production Efficiency ◽  
Haploid Production

scholarly journals Ontogenetic events in androgenesis of Brazilian barley genotypes

2000 ◽  
Vol 60 (2) ◽  
pp. 315-319 ◽  
Author(s):  
A. L. S. da SILVA ◽  
M. I. MORAES-FERNANDES ◽  
A. G. FERREIRA
Keyword(s):  
Hordeum Vulgare ◽  
Doubled Haploid ◽  
Callus Formation ◽  
Simple Procedure ◽  
Pollen Grains ◽  
Hordeum Vulgare L ◽  
Direct Embryogenesis ◽  
Doubled Haploid Plants ◽  
Mode Of Development ◽  
Barley Genotypes

This paper describes a simple procedure for obtention of barley (Hordeum vulgare L.) doubled haploid plants from Brazilian hybrid genotypes. Anatomical and histological examinations showed the reversion of barley pollen to an sporophytic mode of development. A sequence of mitosis led to the formation of multicellular pollen grains. Regeneration of plants occurred either by direct embryogenesis or callus formation followed by differentiation through direct embryogenesis or organogenesis. Plants were formed in the same medium used for induction dispensing an additional regeneration step. This procedure makes doubled haploid production simpler and faster. Plantlets were transferred to another medium for rooting and after that planted in pots with vermiculite and nutrient solution.

scholarly journals Genetic Analyses of Gynogenetic Haploid Production in Onion

2003 ◽  
Vol 128 (4) ◽  
pp. 571-574 ◽  
Author(s):  
Borut Bohanec ◽  
Marijana Jakse ◽  
Michael J. Havey
Keyword(s):  
Allium Cepa ◽  
Doubled Haploid ◽  
Genetic Basis ◽  
Family Selection ◽  
Onion Plant ◽  
Genetic Analyses ◽  
High Frequencies ◽  
Population Effect ◽  
Haploid Production ◽  
Doubled Haploid Plants

The production of doubled haploid plants is desirable as an alternative to sexual inbreeding of longer-generation crops. Onion (Allium cepa L.) is a biennial plant and amenable to the production of gynogenic haploids. Although a strong population effect has been observed for gynogenic haploid production, there is no report describing the genetic basis of greater haploid production in onion. We evaluated over years the frequency of haploid production among onion inbreds and identified lines showing significantly (P < 0.01) greater production of haploids. The onion inbreds, B0223B and B2923B, produced the highest mean frequencies of haploids so far reported. Hybrid families from crosses of B2923B with inbreds having relatively low haploid production showed significantly higher haploid production than the low-producing parent and significantly lower haploid production than B2923B. Plants from B0223B and B2923B with established rates of haploid production were testcrossed and/or self-pollinated. The F1 family from B1717A-1 × B2923B-3 showed rates of haploid production slightly greater than the low parent (B1717A-1) and significantly less than the high parent (B2923B-3). Self-pollination of plants from B2923B showing relatively high rates of haploid production generated S1 progenies also producing relatively high frequencies of haploids. Selfed progenies from plant B2923B-6 showed a high mean rate of haploid production (56.8% ± 14.5%) and, more importantly, the highest level of haploid production (82.2%) reported for any single onion plant. These results indicate that relatively high haploid production, at least for B2923B, was quantitatively inherited with dominance towards low production. We suggest S1 family selection as an effective method to increase gynogenic haploid production of onion populations.

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