A rapid means of identifying wild rice species DNA using dot blots and genome-specific rDNA probes
Keyword(s):
Intergenic spacer fragments from the rDNA repeat unit were isolated from a single accession of each of 9 species that cover the range of genomes found in the Oryza genus (A-F). Seven of the 9 species contained 1 size class of rDNA repeat unit only, while Oryza sativa and Oryza latifolia contained 3 and 2 size classes, respectively, of which fragments were cloned for the major size class only. Oryza australiensis contained an additional BamHI site in the intergenic spacer. Dot blots were prepared and hybridised with a repeat unit from each species. Under high stringency conditions, all probes were specific to species possessing the same genome or genomes.Key words: rDNA, rice, genome-specific, dot blots.
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1995 ◽
Vol 17
(3)
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pp. 197-204
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1985 ◽
Vol 199
(1)
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pp. 154-157
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1990 ◽
Vol 14
(4)
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pp. 381-392
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2000 ◽
Vol 64
(8)
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pp. 1733-1736
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1994 ◽
Vol 41
(6)
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pp. 639-642
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