OBSERVATIONS ON THE USE OF TETRAZOLIUM SALTS IN THE VITAL STAINING OF BACTERIA

1959 ◽  
Vol 5 (3) ◽  
pp. 245-250 ◽  
Author(s):  
L. Eidus ◽  
B. B. Diena ◽  
L. Greenberg
Keyword(s):  
Bacterial Growth ◽  
Vital Staining ◽  
Nitro Blue Tetrazolium ◽  
Complete Inhibition ◽  
Triphenyltetrazolium Chloride ◽  
Tetrazolium Salts ◽  
Blue Tetrazolium

Studies on the vital staining of bacteria were carried out to determine the concentrations of tetrazolium salts required for optimal staining, and the amounts which could cause complete inhibition of bacterial growth. Four salts were studied: neotetrazolium chloride (NTC), triphenyltetrazolium chloride (TTC), blue tetrazolium (BT), and nitro-blue tetrazolium (NBT). Evidence has been presented to show that only living, actively metabolizing cells can reduce neotetrazolium to formazan.

Spectrophotometric determination of browning products of glycation of protein amino groups based on their reactivity with nitro blue tetrazolium salts

The Analyst ◽  
1988 ◽  
Vol 113 (7) ◽  
pp. 1101 ◽  
Author(s):  
Gian Marco Ghiggeri ◽  
Giovanni Candiano ◽  
Fabrizio Ginevri ◽  
Roberta Oleggini ◽  
Maria Teresa Piccardo ◽  
...  
Keyword(s):  
Spectrophotometric Determination ◽  
Nitro Blue Tetrazolium ◽  
Amino Groups ◽  
Tetrazolium Salts ◽  
Blue Tetrazolium

Fructosamine Test-Plus, a modified fructosamine assay evaluated

1991 ◽  
Vol 37 (4) ◽  
pp. 552-556 ◽  
Author(s):  
John Baker ◽  
Patricia Metcalf ◽  
Robert Scragg ◽  
Roger Johnson
Keyword(s):  
Diabetes Mellitus ◽  
Protein Concentration ◽  
Screening Method ◽  
Health Screening ◽  
Disease Prevalence ◽  
Nitro Blue Tetrazolium ◽  
Blue Tetrazolium ◽  
Screening Survey

Abstract We have evaluated Fructosamine Test-Plus, a commercial fructosamine assay based on the reduction of nitro-blue tetrazolium dye in alkaline buffer (Clin Chem 1985;31:1550-4), modified by including a detergent and uricase in the reagent, by changing the concentrations of buffer and dye, and by changing the approach to primary calibration. Specimens were from 2321 participants in a health screening survey in a local workforce. Compared with the original fructosamine method, the Fructosamine Test-Plus method was less affected by protein concentration in the sample and less subject to interference from hyperlipidemia. The changes have also extended the linearity of the assay in the pathological range. However, as a screening method for diabetes mellitus in a population with a disease prevalence of 2.28%, the performance of Fructosamine Test-Plus was similar to the original assay.

ИЗМЕНЕНИЯ ИММУНОЛОГИЧЕСКОГО ОТВЕТА В РЕАКЦИИ НСТ С ПЕРИТОНЕАЛЬНЫМИ МАКРОФАГАМИ ПОД ДЕЙСТВИЕМ ШИРОКОПОЛОСНОГО ЭЛЕКТРОМАГНИТНОГО ИЗЛУЧЕНИЯ В ЭКСПЕРИМЕНТЕ

Innova ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 55-57
Author(s):  
Андрей Сергеевич Руцкой ◽  
◽  
Вячеслав Александрович Липатов ◽  
Александр Анатольевич Панов ◽  
Илья Игоревич Шляпцев ◽  
...  
Keyword(s):  
Immune System ◽  
Enzymatic Activity ◽  
Electromagnetic Fields ◽  
Peritoneal Macrophages ◽  
Nitro Blue Tetrazolium ◽  
Blue Tetrazolium ◽  
Living Organisms ◽  
Different Characteristics

The article presents the results of research on the study of the effect of broadband electromagnetic fields on a macroorganism in an experiment. Electromagnetic fields with different characteristics can affect the systems of living organisms in different ways, including the immune system, which is sensitive to electromagnetic influences. Broadband electromagnetic fields have an immunostimulating effect, as evidenced by the increase in the enzymatic activity of peritoneal macrophages in reaction with nitro blue tetrazolium.

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