Carbon dioxide fixation by cell-free extracts of group D streptococci

1969 ◽  
Vol 15 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Victor F. Lachica ◽  
Paul A. Hartman
Keyword(s):  
Carbon Dioxide ◽  
Pyruvate Carboxylase ◽  
Carbon Dioxide Fixation ◽  
Pep Carboxylase ◽  
Streptococcus Faecium ◽  
Pep Carboxykinase ◽  
Group D

Cell-free extracts of group D streptococci incorporated 14CO2 into aspartate. Pyruvate was the acceptor of CO2 for Streptococcus faecium; phosphoenolpyruvate was the CO2 acceptor for S. bovis; and both substrates were CO2 acceptors for S. faecalis. The enzymes, by which the CO2 fixation was effected, appear to be pyruvate carboxylase for S. faecium, phosphoenolpyruvate (PEP) carboxykinase and PEP carboxylase for S. bovis, and pyruvate carboxylase and PEP carboxykinase for S. faecalis.

CO2 fixation by the facultative autotroph Thiobacillus novellus during autotrophy–heterotrophy interconversions

1974 ◽  
Vol 20 (11) ◽  
pp. 1577-1584 ◽  
Author(s):  
J. T. McCarthy ◽  
A. Michael Charles
Keyword(s):  
Pyruvate Carboxylase ◽  
Co2 Fixation ◽  
Specific Activity ◽  
Mineral Salts ◽  
Original Activity ◽  
Pep Carboxylase ◽  
Growth Decline ◽  
Specific Activities ◽  
Pep Carboxykinase ◽  
Original Amount

During conversion of Thiobacillus novellus to a heterotrophic mode of growth, 14CO2 incorporation declined in freshly grown cells by about 67% of the autotrophic amount within 15 min after transfer to glucose – mineral salts medium. At later times this activity increased until it was about 350% of the original amount. Enzyme assays of freshly prepared crude extracts revealed that initially RuDP carboxylase accounted for 77 ± 4.7% of the total specific activities of the CO2-fixing enzymes studied. PEP carboxylase was 6.7 ± 1.3%; PEP carboxykinase, 8.2 ± 1.8%; and pyruvate carboxylase, 8.2 ± 1.8%. The decline in specific activity of RuDP carboxylase during growth on glucose was relatively slow, accounting for about 8% of the original activity after 18 h. None was detectable after 24 h growth. Decline in the specific activity of phosphoribulokinase and alkaline fructose diphosphatase was also observed. On the other hand, there was an increase in the specific activities of PEP carboxylase, PEP carboxykinase, and pyruvate carboxylase respectively. Conversion of the organism to an autotrophic mode of growth initially resulted in high incorporation of 14CO2. This, however, declined with increased exposure to S2O32− and CO2. The specific activities of the various enzymes also showed a reversal of the patterns noted during heterotrophic development. Phosphoribulokinase reached its maximum (specific activity) earliest. It was followed about 2 h later by RuDP carboxylase, and another 2 h elapsed before alkaline fructose diphosphatase attained its maximum. Finally, S2O32− was found to be necessary for RuDP carboxylase induction; HCO3−, SO32−, or SO32− and HCO3− were ineffective.

CARBON DIOXIDE FIXATION AND PHOSPHOENOLPYRUVATE CARBOXYLASE IN FERROBACILLUS FERROOXIDANS

1967 ◽  
Vol 13 (11) ◽  
pp. 1413-1419 ◽  
Author(s):  
George A. Din ◽  
Isamu Suzuki ◽  
Howard Lees
Keyword(s):  
Carbon Dioxide ◽  
Phosphoenolpyruvate Carboxylase ◽  
Metabolic Regulation ◽  
Carbon Dioxide Fixation ◽  
Acidic Ph ◽  
Ph Optimum ◽  
Carboxylase Activity ◽  
Intact Cells ◽  
Pep Carboxylase

Carbon dioxide fixation was studied in intact cells and cell-free extracts of Ferrobacillus ferrooxidans. The major pathways of fixation were found to be the carboxydismutase system and the phosphoenolpyruvate (PEP) carboxylase system. PEP carboxylase activity was shown to be under metabolic regulation, similar to the regulation established in heterotrophic microorganisms.Acetyl-CoA stimulated PEP carboxylase activity, while aspartate was inhibitory. The F. ferrooxidans enzyme appeared to have a neutral or acidic pH optimum, in contrast to the same enzyme isolated from heterotrophs.

V. CARBON DIOXIDE FIXATION AND SUBSTRATE OXIDATION IN THE CHEMOSYNTHETIC SULFUR AND HYDROGEN BACTERIA

1962 ◽  
Vol 26 (2_Pt_1-2) ◽  
pp. 168-175 ◽  
Author(s):  
Wolf Vishniac ◽  
Philip A. Trudinger
Keyword(s):  
Carbon Dioxide ◽  
Substrate Oxidation ◽  
Carbon Dioxide Fixation ◽  
Hydrogen Bacteria

scholarly journals Chester H. Werkman and Merton F. Utter: Using Bacteria Juice and 13C to Explore Carbon Dioxide Fixation

2005 ◽  
Vol 280 (16) ◽  
pp. e13-e14
Author(s):  
Nicole Kresge ◽  
Robert D. Simoni ◽  
Robert L. Hill
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Fixation

scholarly journals THE SYNTHESIS OF TRICARBOXYLIC ACIDS BY CARBON DIOXIDE FIXATION IN PARSLEY ROOT PREPARATIONS

1949 ◽  
Vol 178 (1) ◽  
pp. 133-143 ◽  
Author(s):  
Joseph. Ceithaml ◽  
Birgit. Vennesland
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Fixation

scholarly journals CARBON DIOXIDE FIXATION IN ISOCITRIC ACID

1947 ◽  
Vol 170 (2) ◽  
pp. 461-465
Author(s):  
Santiago Grisolia ◽  
Birgit Vennesland
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Fixation ◽  
Isocitric Acid

scholarly journals THE PREPARATION OF C14-LABELED BIOTIN AND A STUDY OF ITS STABILITY DURING CARBON DIOXIDE FIXATION

1949 ◽  
Vol 180 (1) ◽  
pp. 299-305 ◽  
Author(s):  
Donald B. Melville ◽  
John G. Pierce ◽  
C.W.H. Partridge
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Fixation

scholarly journals BIOSYNTHESIS OF TRICARBOXYLIC ACIDS BY CARBON DIOXIDE FIXATION

1948 ◽  
Vol 174 (1) ◽  
pp. 123-132 ◽  
Author(s):  
Severo Ochoa ◽  
Erna Weisz-Tabori
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Fixation

scholarly journals BIOSYNTHESIS OF TRICARBOXYLIC ACIDS BY CARBON DIOXIDE FIXATION

1948 ◽  
Vol 174 (1) ◽  
pp. 133-157 ◽  
Author(s):  
Severo Ochoa
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Fixation
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