Sulfur isotope fractionation by Proteus vulgaris and Salmonella heidelberg during the reduction of thiosulfate

1980 ◽  
Vol 26 (10) ◽  
pp. 1173-1177 ◽  
Author(s):  
R. G. L. McCready ◽  
V. A. Grinenko ◽  
H. R. Krouse
Keyword(s):  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Isotope Composition ◽  
Isotopic Fractionation ◽  
Proteus Vulgaris ◽  
Sulfane Sulfur ◽  
Sulfur Isotope Fractionation ◽  
Fractionation Pattern ◽  
High Concentrations ◽  
Salmonella Heidelberg

Proteus vulgaris metabolized thiosulfate to H2S. The amount evolved and its sulfur isotope composition identified it solely with sulfane sulfur. In contrast. Salmonella heidelberg sequentially reduced the sulfane sulfur of S2O32− with slight enrichment of the evolved sulfide in 32S and then reduced the sulfonate sulfur of S2O32− with large isotopic selectivities and an inverse isotopic fractionation pattern. The inverse isotope fractionation pattern for the H2S derived from the sulfonate sulfur was almost identical to that observed during the reduction of high concentrations of sulfite by S. heidelberg.

Sulfur isotope fractionation by Salmonella heidelberg: inverse isotope effects during growth on high concentrations of Na2SO3

1979 ◽  
Vol 25 (12) ◽  
pp. 1387-1393 ◽  
Author(s):  
R. G. L. McCready ◽  
H. R. Krouse
Keyword(s):  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Isotope Effects ◽  
Growth Responses ◽  
Cellular Toxicity ◽  
Sulfur Isotope Fractionation ◽  
High Concentrations ◽  
Salmonella Heidelberg

During growth on minimal salts – glucose media supplemented with high concentrations of Na2SO3 (10−3 and 10−2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at both SO32− concentrations were accompanied by large normal and inverse isotope effects. Consistent with earlier findings, this organism was found capable of rapidly metabolizing both the sulfane and sulfonate sulfur of thiosulfate. Therefore, the isotope effects do not appear to be caused by extracellular chemical thiosulfate formation.

Sulfur isotope fractionation during reduction by different clostridial species

1984 ◽  
Vol 30 (6) ◽  
pp. 841-844 ◽  
Author(s):  
E. J. Laishley ◽  
M. G. Tyler ◽  
H. R. Krouse
Keyword(s):  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Isotope Composition ◽  
Additional Species ◽  
Type Formula ◽  
Sulfur Isotope Fractionation ◽  
Sulfur Isotope Composition ◽  
Clostridial Species

Sulfur isotope composition patterns for sulfide evolved from cultures supplemented with 1 mM Na2SO3, suggest that an inducible dissimilatory type [Formula: see text] reduction pathway, as previously found in C. pasteurianum, probably exists in many clostridial species. Data are presented for five additional species which include pathogens and nonpathogens.

Sulfur isotope fractionation by strains of Alteromonas putrefaciens isolated from oil field fluids

1987 ◽  
Vol 33 (5) ◽  
pp. 372-376 ◽  
Author(s):  
K. M. Semple ◽  
D. W. S. Westlake ◽  
H. R. Krouse
Keyword(s):  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Isotope Composition ◽  
Oil Field ◽  
North Central ◽  
Sulfur Isotope Fractionation ◽  
Sulfur Isotope Composition

Strains of Alteromonas putrefaciens which reduce sulfite and thiosulfate to H2S are readily isolated from oil field fluids in north central Alberta. Data are presented on H2S production during anaerobic sulfite reduction by four strains of A. putrefaciens. Analysis of the sulfur isotope composition (δ34S) of the evolved sulfide shows normal and "inverse" isotope fractionation patterns which are diagnostic of dissimilatory sulfite reduction.

Stable isotope fractionation by Clostridium pasteurianum. 5. Effect of SeO42− on the physiology and associated sulfur isotope fractionation during SO32− and SO42− reductions

1982 ◽  
Vol 28 (3) ◽  
pp. 325-333 ◽  
Author(s):  
G. I. Harrison ◽  
E. J. Laishley ◽  
H. R. Krouse
Keyword(s):  
Stationary Phase ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Reductase Activity ◽  
Clostridium Pasteurianum ◽  
Elemental Selenium ◽  
Whole Cells ◽  
Stable Isotope Fractionation ◽  
Sulfur Isotope Fractionation ◽  
Inverse Isotope Effect

The addition of 1 mM SeO42− significantly affected the physiology and metabolism of Clostridium pasteurianum growing on SO42− in the following ways: (1) the generation time was increased, essentially producing a biphasic growth curve, (2) cells became elongated and chains formed, (3) no H2S was liberated during the stationary phase, (4) assimilatory SO32− reductase activity was decreased, (5) ferredoxin levels decreased by a factor of 4. The effects of 1 mM SeO42− on Clostridium pasteurianum growing on SO32− were comparatively minor.H2S evolution in the stationary phase decreased by a factor of 2 and the δ34S maximum in the inverse isotope effect pattern occurred at a slightly lower percent H2S evolution. The deleterious effects of SeO42− addition were less pronounced than those associated with SeO32− addition. SeO32− but not SeO42− was reduced to elemental selenium by both whole cells and crude extracts.

Sulfur Isotope Fractionation by Sulfate-Reducing Microbes Can Reflect Past Physiology

2018 ◽  
Vol 52 (7) ◽  
pp. 4013-4022 ◽  
Author(s):  
André Pellerin ◽  
Christine B. Wenk ◽  
Itay Halevy ◽  
Boswell A. Wing
Keyword(s):  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Sulfate Reducing ◽  
Sulfur Isotope Fractionation
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