Actions antibiotique, mycolytique et parasitaire de deux actinomycètes envers Fusarium oxysporum f.sp. albedinis et autres formae speciales

1983 ◽  
Vol 29 (2) ◽  
pp. 194-199 ◽  
Author(s):  
Nasserdine Sabaou ◽  
Nicole Bounaga ◽  
Djilali Bounaga

Two actinomycètes (X9 and X10) isolated on a Fusarium oxysporum f.sp. albedinis strain showed antibiotic, mycolytic, and parasitism actions against nine "formae speciales" of Fusarium oxysporum. Strain X10 is included in genus Streptomyces and strain X9 in Nocardiopsis dassonvillei species (Lechevalier and Lechevalier) Meyer. The antibiotic action of Streptomyces strain is stronger towards F. o. dianthi, F. o. albedinis, and F. o. cyclaminis. It seems that the mycolytic power of N. dassonvillei is more specific to F. o. eleaidis, whereas the parasitism action varies depending on the "formae speciales" tested and the quantity of fungal mycelium produced. As a response to this parasitism, all the fungi produce resistance organs that can either be polymorphous thallospores or chlamydospores. The thallospores studied on a F. o. albedinis strain germinate faster than the microconidies of the same nonparasited fungus. However, many strains obtained from these thallospores are as susceptible as the mother strain to the antagonistic action of both actinomycetes




2008 ◽  
Vol 24 ◽  
pp. 1481 ◽  
Author(s):  
Eiko Eurya Kuramae ◽  
Nilton Luiz De Souza

Diferenciação de formae speciales e raças de Fusarium oxysporum através de características morfológicas e culturais é bastante difícil e muitas vezes duvidosa. Marcadores moleculares RAPD e sequenciamento das regiões ITS1 e ITS2 (internal transcribed spacer) e o gene 5.8S rDNA foram utilizados para estimar a variabilidade genética existente entre quatro formae speciales de Fusarium oxysporum (F. o. cubense, F. o. lycopersici, F. o. phaseoli and F. o. vasinfectum) e entre 2 raças de F. o. lycopersici. A variabilidade genética em nível de DNA genômico utilizando-se marcadores moleculares RAPD foi de até 50% entre as formae speciales e, entre as raças 1 e 2 de F. o. lycopersici foi de 7%. Houve similaridade maior que 97,2% entre as diferentes formae speciales e raças fisiológicas de F. o lycopersici na regiões correspondentes a ITS1 e ITS2, enquanto que o gene 5.8S rDNA apresentou 100% de homologia entre os isolados





2015 ◽  
Vol 78 ◽  
pp. 263-269 ◽  
Author(s):  
Miguel A. Gómez-Tenorio ◽  
María J. Zanón ◽  
Miguel de Cara ◽  
Beatriz Lupión ◽  
Julio C. Tello


2000 ◽  
Vol 90 (8) ◽  
pp. 891-900 ◽  
Author(s):  
Robert P. Baayen ◽  
Kerry O'Donnell ◽  
Peter J. M. Bonants ◽  
Elizabeth Cigelnik ◽  
Laurens P. N. M. Kroon ◽  
...  

The monophyletic origin of host-specific taxa in the plant-pathogenic Fusarium oxysporum complex was tested by constructing nuclear and mitochondrial gene genealogies and amplified fragment length polymorphism (AFLP)-based phylogenies for 89 strains representing the known genetic and pathogenic diversity in 8 formae speciales associated with wilt diseases and root and bulb rot. We included strains from clonal lineages of F. oxysporum f. spp. asparagi, dianthi, gladioli, lilii, lini, opuntiarum, spinaciae, and tulipae. Putatively nonpathogenic strains from carnation and lily were included and a reference strain from each of the three main clades identified previously in the F. oxysporum complex; sequences from related species were used as outgroups. DNA sequences from the nuclear translation elongation factor 1α and the mitochondrial small subunit (mtSSU) ribosomal RNA genes were combined for phylogenetic analysis. Strains in vegetative compatibility groups (VCGs) shared identical sequences and AFLP profiles, supporting the monophyly of the two single-VCG formae speciales, lilii and tulipae. Identical genotypes were also found for the three VCGs in F. oxysporum f. sp. spinaciae. In contrast, multiple evolutionary origins were apparent for F. oxysporum f. spp. asparagi, dianthi, gladioli, lini, and opuntiarum, although different VCGs within each of these formae speciales often clustered close together or shared identical EF-1α and mtSSU rDNA haplotypes. Kishino-Hasegawa analyses of constraints forcing the monophyly of these formae speciales supported the exclusive origin of F. oxysporum f. sp. opuntiarum but not the monophyly of F. oxysporum f. spp. asparagi, dianthi, gladioli, and lini. Most of the putatively nonpathogenic strains from carnation and lily, representing unique VCGs, were unrelated to F. oxysporum f. spp. dianthi and lilii, respectively. Putatively nonpathogenic or rot-inducing strains did not form exclusive groups within the molecular phylogeny. Parsimony analyses of AFLP fingerprint data supported the gene genealogy-based phylogram; however, AFLP-based phylogenies were considerably more homoplasious than the gene genealogies. The predictive value of the forma specialis naming system within the F. oxysporum complex is questioned.





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