Restriction fragment profiles of genome segment A of infectious bursal disease virus correlate with serotype and geographical origin of avibirnaviruses

1996 ◽  
Vol 42 (1) ◽  
pp. 93-97 ◽  
Author(s):  
B. Qian ◽  
F. S. B. Kibenge
Keyword(s):  
Disease Virus ◽  
Restriction Fragment ◽  
Infectious Bursal Disease Virus ◽  
Geographical Origin ◽  
Genome Segment ◽  
Amino Acid Sequences ◽  
Infectious Bursal Disease ◽  
Genomic Segment ◽  
Bursal Disease ◽  
Serotype 1

Previous analyses of the two serotypes of infectious bursal disease virus (IBDV) have demonstrated the correlation between antigenicity and similarities of nucleotide and amino acid sequences of the VP2 coding region in genome segment A. Restriction fragment profiles of genomic segment A cDNA of five IBDV isolates (QC-2 and QT-1 of serotype 1, SK9, and Nos. 39 and 52 of serotype 2) were determined in order to establish the genetic relationship of these viruses to other avibirnaviruses. The restriction fragment profiles using three of seven restriction enzymes (Sad which cuts in the VP2 region, DraIII which cuts in the VP3 region, and EcoRI which cuts in the VP4 region) were used to place QC-2, QT-1, SK9, No. 39, and No. 52 within the phylogenetic tree among seven other avibirnaviruses of known sequence. The two IBDV serotypes corresponded to two genotypes on the basis of the presence or absence of the SacI restriction site. The serotype 1 cluster of strains was further differentiated into five minor clusters on the basis of the PstI, EcoRI, BamHI, HindIII, DraIII, and Bsu36I restriction sites, which emphasized the geographical origins of the strains. It is concluded that restriction analysis of cDNA of the whole viral genomic segment A allows differentiation of IBDV isolates on the basis of their antigenicity and geographical origin.Key words: phylogeny, avibirnavirus, geographical origin.

scholarly journals Genomic sequence of infectious bursal disease virus from Zambia suggests evidence for genome re-assortment in nature

2012 ◽  
Vol 79 (2) ◽  
Author(s):  
Christopher J. Kasanga ◽  
T. Yamaguchi ◽  
H.M. Munang’andu ◽  
P.N. Wambura ◽  
K. Ohya ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Genomic Sequence ◽  
Rna Virus ◽  
Virulent Strain ◽  
Amino Acid Sequences ◽  
Infectious Bursal Disease ◽  
Nucleotide Homology ◽  
Reassortant Strain ◽  
Bursal Disease

Infectious bursal disease virus (IBDV) is a bi-segmented RNA virus, which belongs to the genus Avibirnavirus of the family Birnaviridae. Two serotypes, 1 and 2, exist in IBDV. The serotype 1 IBDVs are the causative agents of infectious bursal disease (IBD) in chickens worldwide and lead to immunosuppression in young birds. Genome re-assortment has been speculated to occur and contribute to the emergence of new IBDV strains. However, evidence was lacking until recently when two re-assortant viruses were detected in China. In this study, we determined the complete nucleotide sequence of an IBDV, designated KZC-104, from a confirmed natural IBD outbreak in Lusaka, Zambia in 2004. The genome consisted of 3074 and 2651 nucleotides in the coding regions of segments A and B, respectively. Alignment of both nucleotide and deduced amino acid sequences, and phylogenetic analysis revealed that the genome segment A of KZC-104 was derived from a very virulent strain, whereas its segment B was derived from a classical attenuated strain. On BLAST search, the full-length segments A and B sequences showed 98% closest nucleotide homology to the very virulent strain D6948 and 99.8% closest nucleotide homology to the classical attenuated strain D78, respectively. This is a unique IBDV reassortant strain, which has emerged in nature involving segment B of a live attenuated vaccine. This observation provides direct evidence for the involvement of vaccine strains in the emergence of reassortant IBDV in the field. Taken together, these findings suggest an additional risk of using live IBDV vaccines, which may act as genetic donors for genome re-assortment. Further studies are required to investigate the epidemiology and biological characteristics of reassortant strains so that the appropriate and safe IBDV vaccines can be recommended.

scholarly journals Chemiluminescent Detection of Infectious Bursal Disease Virus with a PCR-Generated Nonradiolabeled Probe

1993 ◽  
Vol 5 (2) ◽  
pp. 166-173 ◽  
Author(s):  
A. Akin ◽  
C. C. Wu ◽  
T. L. Lin ◽  
R. W. Keirs
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Salmonella Enteritidis ◽  
Blot Hybridization ◽  
Infectious Bursal Disease ◽  
Slot Blot ◽  
Slot Blot Hybridization ◽  
Bursal Disease ◽  
Serotype 1 ◽  
Labeled Probe

A polymerase chain reaction (PCR)-generated digoxigenin-labeled nonradioactive oligonucleotide probe was developed and utilized in slot-blot hybridization coupled with chemiluminescence for the detection of infectious bursal disease virus (IBDV). The probe was prepared from the RNA of the standard challenge strain (STC) of IBDV serotype 1 by reverse transcription followed by 2 PCR amplifications with 2 separate sets of primers. RNA of STC viruses prepared from bursae infected with STC viruses was subjected to the first PCR with the outer primers V8 and V9 that amplified a 607-base pair (bp) segment. The PCR product from the first PCR was eluted following agarose gel electrophoresis and subjected to the second PCR with the nested primers V6 and V7 that flanked a 351-bp segment. In the second PCR, dTTP was substituted by digoxigenin-11-dUTP in the PCR reaction mixture so that the amplified 351-bp DNA products were labeled with digoxigenin. The specificity of the PCR-generated digoxigenin-labeled probe was tested on different strains of IBDV, several unrelated avian viruses, and bacteria by slot-blot hybridization assay. Hybridization was detected by chemiluminescence. The sensitivity of the probe was assayed using lo-fold serial dilutions of purified RNA from the STC strain of IBDV. The PCR-generated digoxigenin-labeled probe hybridized with genomic RNA of STC and variant strains A, D, E, G, and GLS-5 of IBDV serotype 1 but not OH strain of IBDV serotype 2. The probe did not react with avian reovirus, infectious bronchitis virus, Salmonella enteritidis, Escherichia coli, or Staphylococcus aureus. The probe was very sensitive, and as little as 72 fg of RNA from the STC strain of IBDV could be detected. The results indicate that this PCR-generated digoxigenin-labeled nonisotopic probe is specific for IBDV and may be utilized in a diagnostic assay for all IBDV serotype 1 strains.

scholarly journals Phylogenetic Analysis Reveals a Correlation between the Expansion of Very Virulent Infectious Bursal Disease Virus and Reassortment of Its Genome Segment B

2006 ◽  
Vol 80 (17) ◽  
pp. 8503-8509 ◽  
Author(s):  
Chung-Chau Hon ◽  
Tsan-Yuk Lam ◽  
Alexei Drummond ◽  
Andrew Rambaut ◽  
Yiu-Fai Lee ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Population Dynamics ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Genome Segment ◽  
Infectious Bursal Disease ◽  
Reassortment Event ◽  
The Past ◽  
Bursal Disease ◽  
Past Population

ABSTRACT Infectious bursal disease virus (IBDV) is a birnavirus causing immunosuppressive disease in chickens. Emergence of the very virulent form of IBDV (vvIBDV) in the late 1980s dramatically changed the epidemiology of the disease. In this study, we investigated the phylogenetic origins of its genome segments and estimated the time of emergence of their most recent common ancestors. Moreover, with recently developed coalescence techniques, we reconstructed the past population dynamics of vvIBDV and timed the onset of its expansion to the late 1980s. Our analysis suggests that genome segment A of vvIBDV emerged at least 20 years before its expansion, which argues against the hypothesis that mutation of genome segment A is the major contributing factor in the emergence and expansion of vvIBDV. Alternatively, the phylogeny of genome segment B suggests a possible reassortment event estimated to have taken place around the mid-1980s, which seems to coincide with its expansion within approximately 5 years. We therefore hypothesize that the reassortment of genome segment B initiated vvIBDV expansion in the late 1980s, possibly by enhancing the virulence of the virus synergistically with its existing genome segment A. This report reveals the possible mechanisms leading to the emergence and expansion of vvIBDV, which would certainly provide insights into the scope of surveillance and prevention efforts regarding the disease.

Generation of serotype 1/serotype 2 reassortant viruses of the infectious bursal disease virus and their investigation in vitro and in vivo

2004 ◽  
Vol 105 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Kati Zierenberg ◽  
Rüdiger Raue ◽  
Hermann Nieper ◽  
Md.Rafiqul Islam ◽  
Nicolas Eterradossi ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Serotype 1 ◽  
Reassortant Viruses
Sign in / Sign up

Export Citation Format

Share Document