Transgenic larch expressing genes for herbicide and insect resistance

1994 ◽  
Vol 24 (10) ◽  
pp. 2059-2067 ◽  
Author(s):  
Dong-Ill Shin ◽  
Gopi K. Podila ◽  
Yinghua Huang ◽  
David F. Karnosky
Keyword(s):  
Gene Transfer ◽  
Binary Vector ◽  
Value Added ◽  
Adventitious Shoot ◽  
Direct Organogenesis ◽  
Toxin Gene ◽  
European Larch ◽  
Adventitious Shoot Buds ◽  
Selection Medium ◽  
Aroa Gene

Transgenic European larch (Larixdecidua Mill.) plants expressing a Bacillusthuringiensis Berliner (B.t.) toxin gene or the glyphosate tolerance (aroA) gene have been produced using Agrobacteriumrhizogenes mediated gene transfer. This procedure relies on direct organogenesis on wounded hypocotyls following A. rhizogenes infection. Hypocotyls of seven-day-old larch seedlings were inoculated with A. rhizogenes strain 11325, harboring the oncogenic nopaline-type pRi11325 and either binary vector pCGN1133 containing 35S NPTII and 35S ssu/aroA or pWB139 containing 35S NPTII–B.t. gene. Adventitious shoot buds were induced 4 weeks after infection. Shoots were excised, elongated, and rooted on selection medium containing kanamycin. Needles from greenhouse-grown plants were confirmed to have and to express the B.t. or aroA gene through Southern, Northern, and Western blot analyses and bioassays. This is the first report of regeneration of transgenic conifer plants expressing value-added genes using Agrobacterium-mediated gene transfer.

scholarly journals Development of an in vitro Callus Induction Protocol and Shoot Proliferation for Selected Jatropha (Jatropha curcas) Accessions

2020 ◽  
Vol 30 (1) ◽  
pp. 131-141
Author(s):  
Hundessa Fufa ◽  
Jiregna Daksa
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Jatropha Curcas ◽  
Plant Tissue ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Regeneration Medium ◽  
Adventitious Shoot Buds

The present study was undertaken to establish a protocol for in vitro callusing of three Jatropha accessions, namely Metema, Adami Tulu and Shewa Robit from leaf explants. The medium supplemented with combination of 4.44 μM BAP and 4.52 μM 2,4-D resulted in maximum percentage of callus (100%) formed for all accessions. The maximum shoot regeneration (66.67%) from callus with 10.13 number of shoot was obtained from Shewa Robit in MS medum fortified with TDZ (2.27 μM ) and IBA (0.49 μM ). The presence of TDZ in the shoot regeneration medium has greater influence on the induction of adventitious shoot buds, whereas MS supplemented with BAP alone and combination with IBA did not induce shoot regeneration from callus culture. The results obtained in the present study would facilitate the high callus induction and regeneration responses in Jatropha for its improvement using biotechnological tools. Plant Tissue Cult. & Biotech. 30(1): 131-141, 2020 (June)

Improved efficiency of uidA gene transfer in stone pine (Pinus pinea) cotyledons using a modified binary vector

1999 ◽  
Vol 29 (10) ◽  
pp. 1627-1632 ◽  
Author(s):  
Jaime M. Humara ◽  
M. Soledad Marín ◽  
Francisco Parra ◽  
Ricardo J. Ordás
Keyword(s):  
Gene Transfer ◽  
Binary Vector ◽  
Pinus Pinea ◽  
Stone Pine ◽  
I Gene

scholarly journals Horizontal gene transfer of a bacterial insect toxin gene into the Epichloë fungal symbionts of grasses

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Karen V. Ambrose ◽  
Albrecht M. Koppenhöfer ◽  
Faith C. Belanger
Keyword(s):  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Toxin Gene ◽  
Insect Toxin

Agrobacterium-Mediated gene transfer using binary vector in lettuce (Lactuca sativa L.)

2018 ◽  
Vol 55 (3) ◽  
pp. 443 ◽  
Author(s):  
Ishani shaunak ◽  
Pankaj Kumar ◽  
Ayesh Gaur ◽  
Shikha Sharma ◽  
Dinesh K Srivastava
Keyword(s):  
Gene Transfer ◽  
Lactuca Sativa ◽  
Binary Vector ◽  
Lactuca Sativa L

scholarly journals Repression of YdaS Toxin is Mediated by Transcriptional Repressor RacR in the cryptic rac prophage of Escherichia coli-K12

2017 ◽  
Author(s):  
Revathy Krishnamurthi ◽  
Swagatha Ghosh ◽  
Supriya Khedkar ◽  
Aswin Sai Narain Seshasayee
Keyword(s):  
Escherichia Coli ◽  
Transcription Factor ◽  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Driving Force ◽  
Transcriptional Repressor ◽  
Genomic Diversity ◽  
Toxin Gene ◽  
Escherichia Coli K12 ◽  
Putative Transcription Factor

AbstractHorizontal gene transfer is a major driving force behind the genomic diversity seen in prokaryotes. Theracprophage inE.coliK12 encodes a putative transcription factor RacR, whose deletion is lethal. We have shown that the essentiality ofracRinE.coliK12 is attributed to its role in transcriptionally repressing a toxin gene calledydaS, which is coded adjacent and divergently toracR.

scholarly journals Agrobacterium-mediated Transformation of Cotyledon Explants of Bottle Gourd (Lagenaria siceraria S.)

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 821E-822
Author(s):  
Jeung-Sul Han* ◽  
Chang Kil Kim
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Binary Vector ◽  
Bottle Gourd ◽  
Infection Frequency ◽  
Ms Medium ◽  
Lagenaria Siceraria ◽  
Cultivation Medium ◽  
Glufosinate Ammonium ◽  
Cotyledon Explants ◽  
Selection Medium

A procedure for producing transgenic bottle gourd plants by inoculating cotyledon explants with Agrobacterium tumefaciens strain AGL1 carrying a binary vector pCAMBIA3301, which contains glufosinate ammonium-resistant (bar) and the reporter (gus) genes, is describe. Infection was the most effective (highest infection frequency and index) when explants were co-cultivated with Agrobacterium for 6-8 days on co-cultivation medium supplemented with 0.001-0.1 mg/L L-a-(2-aminoethoxyvinyl) glycine (AVG). Transgenic plants were obtained with frequencies of about 0.2% when the explants were cultured on selection medium (MS medium supplemented with 3.0 mg/L BAP, 0.5 mg/L AgNO3, 500 mg/L cefotaxime, 2.0 mg/L DL-phosphinothricin, 0.3% sucrose and 0.8% Plant Agar. A histochemical gus assay, PCR and Southern blot analyses confirmed that transformation had occurred. Genetic analysis of T1 progenies showed that the transgenes were inherited in a Mendelian fashion. To our knowlege, this study represents the first report for Agrobacterium-mediated transformation in bottle gourd, rootstock for watermelon and other cucurbit crops in many countries.

scholarly journals ANATOMICAL STUDIES OF IN VITRO ORGANOGENESIS INDUCED IN LEAF-DERIVED EXPLANTS OF PASSIONFRUIT

1999 ◽  
Vol 34 (11) ◽  
pp. 2007-2013 ◽  
Author(s):  
BEATRIZ APPEZZATO DA GLORIA ◽  
MARIA LUCIA CARNEIRO VIEIRA ◽  
MARCELO CARNIER DORNELAS
Keyword(s):  
De Novo ◽  
Adventitious Shoot ◽  
Direct Shoot Regeneration ◽  
Continuous Darkness ◽  
Passiflora Edulis ◽  
Anatomical Studies ◽  
Adventitious Shoot Buds ◽  
Organogenesis In Vitro ◽  
Direct Shoot

With the aim of studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of parenchymal origin.

REGENERATION OF PLANTLETS FROM HYPOCOTYL DERIVED CALLUS OF MORUS ALBA

1995 ◽  
Vol 43 (3) ◽  
pp. 259-262 ◽  
Author(s):  
K. Kathiravan ◽  
A. Shajahan ◽  
A. Ganapathi
Keyword(s):  
Indoleacetic Acid ◽  
Adventitious Shoot ◽  
Morus Alba ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Shoot Buds ◽  
Adventitious Shoot Buds ◽  
Hypocotyl Segments

Plantlets were regenerated from hypocotyl callus of Morus alba cv. MR2. Calli were established from hypocotyl segments on Murashige and Skoog (MS) medium supplemented with indoleacetic acid (0.5 mg/1) and benzyladenine (BA) (0.5 mg/1). They were transferred to MS medium with different concentrations of naphthaleneacetic acid NAA and BA for four weeks. Adventitious shoot buds were observed by transferring callus onto fresh Linsmaier and Skoog (LS) medium containing NAA (0.5 mg/1) and BA (0.75 mg/1). Shoots produced in vitro were rooted on MS medium with indolebutyric acid (0.75 mg/1).

scholarly journals In Vitro Regeneration through Direct Organogenesis from Protocorms of Oncidium tigrinum Llave & Lex. (Orchidaceae), an Endemic and Threatened Mexican Species

HortScience ◽  
2011 ◽  
Vol 46 (8) ◽  
pp. 1132-1135 ◽  
Author(s):  
Martín Mata-Rosas ◽  
Rosario Julieta Baltazar-García ◽  
Victor Manuel Chávez-Avila
Keyword(s):  
Culture Media ◽  
In Vitro Regeneration ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Average Height ◽  
Ms Medium ◽  
Adventitious Shoot Formation

A protocol for in vitro propagation from protocorms of Oncidium tigrinum Llave & Lex., a threatened species distributed in Mexico and highly appreciated as an ornamental, was developed. Two different explants, entire protocorms and longitudinal halves of protocorms, were used. In addition, the effect of two different culture media, Murashige and Skoog (MS) and modified Knudson (KCm), supplemented with N6-benzyladenine (BA) (0, 0.5, 1, 2, 3, and 5 mg·L−1) and/or α-naphthaleneacetic acid (NAA) at 0, 0.1, and 0.5 mg·L−1 was investigated. Adventitious shoot formation by direct organogenesis was obtained in all treatments; in some cases, the formation of protocorm-like bodies was induced. Shoot formation was greater for entire protocorms; the best treatment was MS medium containing at BA 1 to 2 mg·L−1 in combination with at NAA 0.1 mg·L−1. The average height of shoots was three times greater in MS medium than in KCm medium. Subculturing individual shoots in MS medium without plant growth regulators, but with 1 g·L−1 activated charcoal, allowed further development and rooting. An ex vitro survival rate of almost 100% was achieved. This study represents a comprehensive application for propagation, conservation, and sustainable use of this valuable natural resource.

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