A Model System for Gene Transfer in Conifers: European Larch and Agrobacterium

1988 ◽  
pp. 55-63 ◽  
Author(s):  
D. F. Karnosky ◽  
A. M. Diner ◽  
W. M. Barnes
Keyword(s):  
Gene Transfer ◽  
Model System ◽  
European Larch

Transgenic larch expressing genes for herbicide and insect resistance

1994 ◽  
Vol 24 (10) ◽  
pp. 2059-2067 ◽  
Author(s):  
Dong-Ill Shin ◽  
Gopi K. Podila ◽  
Yinghua Huang ◽  
David F. Karnosky
Keyword(s):  
Gene Transfer ◽  
Binary Vector ◽  
Value Added ◽  
Adventitious Shoot ◽  
Direct Organogenesis ◽  
Toxin Gene ◽  
European Larch ◽  
Adventitious Shoot Buds ◽  
Selection Medium ◽  
Aroa Gene

Transgenic European larch (Larixdecidua Mill.) plants expressing a Bacillusthuringiensis Berliner (B.t.) toxin gene or the glyphosate tolerance (aroA) gene have been produced using Agrobacteriumrhizogenes mediated gene transfer. This procedure relies on direct organogenesis on wounded hypocotyls following A. rhizogenes infection. Hypocotyls of seven-day-old larch seedlings were inoculated with A. rhizogenes strain 11325, harboring the oncogenic nopaline-type pRi11325 and either binary vector pCGN1133 containing 35S NPTII and 35S ssu/aroA or pWB139 containing 35S NPTII–B.t. gene. Adventitious shoot buds were induced 4 weeks after infection. Shoots were excised, elongated, and rooted on selection medium containing kanamycin. Needles from greenhouse-grown plants were confirmed to have and to express the B.t. or aroA gene through Southern, Northern, and Western blot analyses and bioassays. This is the first report of regeneration of transgenic conifer plants expressing value-added genes using Agrobacterium-mediated gene transfer.

scholarly journals Gene transfer to carcinoma of the breast with fiber-modified adenoviral vectors in a tissue slice model system

2005 ◽  
Vol 4 (11) ◽  
pp. 1203-1210 ◽  
Author(s):  
Mariam A. Stoff-Khalili ◽  
Alexander Stoff ◽  
Angel A Rivera ◽  
J. Michael Mathis ◽  
Maaike Everts ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Adenoviral Vectors ◽  
Model System ◽  
Tissue Slice ◽  
Carcinoma Of The Breast

A model system for in vivo gene transfer into the central nervous system using an adenoviral vector

1993 ◽  
Vol 3 (3) ◽  
pp. 219-223 ◽  
Author(s):  
Beverly L. Davidson ◽  
Edward D. Allen ◽  
Karen F. Kozarsky ◽  
James M. Wilson ◽  
Blake J. Roessler
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Gene Transfer ◽  
Adenoviral Vector ◽  
Model System ◽  
The Central Nervous System ◽  
In Vivo Gene Transfer

scholarly journals A Novel Biofilm Model System to Visualise Conjugal Transfer of Vancomycin Resistance by Environmental Enterococci

2021 ◽  
Vol 9 (4) ◽  
pp. 789
Author(s):  
Michael Conwell ◽  
James S. G. Dooley ◽  
Patrick J. Naughton
Keyword(s):  
Antibiotic Resistance ◽  
Gene Transfer ◽  
Antibiotic Resistance Gene ◽  
In Situ Hybridisation ◽  
Model System ◽  
Vancomycin Resistance ◽  
Conjugal Transfer ◽  
Clinical Settings ◽  
Resistance Transfer ◽  
Non Destructive

Enterococci and biofilm-associated infections are a growing problem worldwide, given the rise in antibiotic resistance in environmental and clinical settings. The increasing incidence of antibiotic resistance and its propagation potential within enterococcal biofilm is a concern. This requires a deeper understanding of how enterococcal biofilm develops, and how antibiotic resistance transfer takes place in these biofilms. Enterococcal biofilm assays, incorporating the study of antibiotic resistance transfer, require a system which can accommodate non-destructive, real-time experimentation. We adapted a Gene Frame® combined with fluorescence microscopy as a novel non-destructive platform to study the conjugal transfer of vancomycin resistance in an established enterococcal biofilm.A multi-purpose fluorescent in situ hybridisation (FISH) probe, in a novel application, allowed the identification of low copy number mobile elements in the biofilm. Furthermore, a Hoechst stain and ENU 1470 FISH probe identified Enterococcus faecium transconjugants by excluding Enterococcus faecalis MF06036 donors. Biofilm created with a rifampicin resistant E. faecalis (MW01105Rif) recipient had a transfer efficiency of 2.01 × 10−3; double that of the biofilm primarily created by the donor (E. faecalis MF06036). Conjugation in the mixed enterococcal biofilm was triple the efficiency of donor biofilm. Double antibiotic treatment plus lysozyme combined with live/dead imaging provided fluorescent micrographs identifying de novo enterococcal vancomycin resistant transconjugants inside the biofilm. This is a model system for the further study of antibiotic resistance transfer events in enterococci. Biofilms promote the survival of enterococci and reduce the effectiveness of drug treatment in clinical settings, hence giving enterococci an advantage. Enterococci growing in biofilms exchange traits by means of horizontal gene transfer, but currently available models make study difficult. This work goes some way to providing a non-destructive, molecular imaging-based model system for the detection of antibiotic resistance gene transfer in enterococci.

A Protozoan Model for Golgi-Associated Calcification

1971 ◽  
Vol 29 ◽  
pp. 332-333
Author(s):  
D. C. Williams ◽  
D. E. Outka
Keyword(s):  
Golgi Apparatus ◽  
Organic Matrix ◽  
Model System ◽  
Sequential Formation

Many studies have shown that the Golgi apparatus is involved in a variety of synthetic activities, and probably no Golgi product is more elaborate than the scales produced by various kinds of phytoflagellates. The formation of calcified scales (coccoliths, Fig. 1,2) of the coccolithophorid phytoflagellates provides a particularly interesting model system for the study of biological mineralization, and the sequential formation of Golgi products.The coccoliths of Hymenomonas carterae consist of a scale-like base (Fig. 2 and 4, b) with a highly structured calcified (CaCO3) rim composed of two distinct elements which alternate about the base periphery (Fig. 1 and 3, A, B). Each element is enveloped by a sheath-like organic matrix (Fig. 3; Fig. 4, m).

Existence of DNA in yeast microbodies

1978 ◽  
Vol 36 (2) ◽  
pp. 232-233
Author(s):  
Masako Osumi ◽  
Misuzu Nagano ◽  
Hiroko Kazama
Keyword(s):  
Catalase Activity ◽  
Buoyant Density ◽  
Model System ◽  
Contour Length ◽  
Native State ◽  
Normal Alkane ◽  
Remarkable Increase ◽  
Dna Molecule ◽  
Mitochondrial Dnas

We have found that microbodies appeared profusely together with a remarkable increase in catalase activity in normal alkane-grown cells of hydrocarbon-utilizing Candida yeasts, and that the microbodies multiplied by division in these cells. These features of Candida yeasts seem to provide a useful model system for studies on the biogenesis of the microbody. Subsequently, we have succeeded in isolation of Candida microbodies in an apparently native state, as judged biochemically and morphologically. The presence of DNA in the purified microbody fraction thus obtained was proved by the diphenylamine method. DNA molecule of about 15 urn in contour length was released from an isolated microbody. The physicochemical analyses of the microbody DNA revealed that its buoyant density differed from nuclear and mitochondrial DNAs. All these results lead us to the possibility that there is a novel type of DNA in microbodies.

The coprecipitation of Cr3P and Cr in Cu

1988 ◽  
Vol 46 ◽  
pp. 764-765
Author(s):  
M.J. Witcomb ◽  
U. Dahmen ◽  
K.H. Westmacott
Keyword(s):  
Orientation Relationship ◽  
Interface Structure ◽  
Model System ◽  
Relative Orientation ◽  
Age Hardening ◽  
Precipitation Behavior ◽  
Solution Treated ◽  
Small Needle ◽  
Diffraction Patterns ◽  
Interface Structures

Cu-Cr age-hardening alloys are of interest as a model system for the investigation of fcc/bcc interface structures. Several past studies have investigated the morphology and interface structure of Cr precipitates in a Cu matrix (1-3) and good success has been achieved in understanding the crystallography and strain contrast of small needle-shaped precipitates. The present study investigates the effect of small amounts of phosphorous on the precipitation behavior of Cu-Cr alloys.The same Cu-0.3% Cr alloy as was used in earlier work was rolled to a thickness of 150 μm, solution treated in vacuum at 1050°C for 1h followed by quenching and annealing for various times at 820 and 863°C.Two laths and their corresponding diffraction patterns in an alloy aged 2h at 820°C are shown in correct relative orientation in Fig. 1. To within the limit of accuracy of the diffraction patterns the orientation relationship was that of Kurdjumov-Sachs (KS), i.e. parallel close-packed planes and directions.

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