The Further Purification of Motilin, a Gastric Motor Activity Stimulating Polypeptide from the Mucosa of the Small Intestine of Hogs

1971 ◽  
Vol 49 (5) ◽  
pp. 399-405 ◽  
Author(s):  
John C. Brown ◽  
Victor Mutt ◽  
Jill R. Dryburgh
Keyword(s):  
Small Intestine ◽  
Motor Activity ◽  
Glutamic Acid ◽  
Duodenal Mucosa ◽  
The Other ◽  
Fundic Gland ◽  
Terminal Residue ◽  
Gastric Motor Activity ◽  
Gland Area ◽  
Fundic Gland Area

A polypeptide has been isolated from the duodenal mucosa of hogs and has been named motilin. Motilin stimulates motor activity in both antral and fundic gland area pouches of the stomach of dogs. It will stimulate pepsin output, with no change in H+ secretion, from fundic gland area pouches. Motilin differs from the other characterized gastrointestinal polypeptides both chemically and in its physiological actions. In particular, the presence of phenylalanine as N-terminal residue, the absence of histidine, and presence of large amounts of glutamic acid and glutamine distinguish it from cholecystokinin–pancreozymin and secretin.

Gastric ulcer and carcinoma in fundic gland area

1975 ◽  
Vol 10 (4) ◽  
pp. 290-299
Author(s):  
Hiroshi Yamagiwa ◽  
Akinori Ishihara ◽  
Minoru Hamazaki
Keyword(s):  
Gastric Ulcer ◽  
Fundic Gland ◽  
Gland Area ◽  
Fundic Gland Area

TRH stimulation andl-glutamic acid inhibition of proximal gastric motor activity in the rat dorsal vagal complex

1989 ◽  
Vol 495 (2) ◽  
pp. 319-328 ◽  
Author(s):  
H.E. Raybould ◽  
L.J. Jakobsen ◽  
D. Novin ◽  
Y. Taché
Keyword(s):  
Motor Activity ◽  
Glutamic Acid ◽  
Acid Inhibition ◽  
Dorsal Vagal Complex ◽  
Gastric Motor Activity ◽  
Trh Stimulation

Intestinal metaplasia of the stomach confined to the fundic gland area. Report of two cases

1987 ◽  
Vol 17 (4) ◽  
pp. 276-280
Author(s):  
Masashi Hirono ◽  
Koichi Mandai ◽  
Tetsuya Toge ◽  
Minoru Niimoto ◽  
Takao Hattori ◽  
...  
Keyword(s):  
Intestinal Metaplasia ◽  
Fundic Gland ◽  
Gland Area ◽  
Fundic Gland Area

Clinical and histochemical studies on early and early simulating advanced carcinoma located in the fundic gland area of the stomach.

1989 ◽  
Vol 22 (10) ◽  
pp. 2338-2343
Author(s):  
Michio SOWA ◽  
Yasuyuki KATO ◽  
Masanori NISHIMURA ◽  
Toshiaki KUBO ◽  
Hitoshi MAEKAWA ◽  
...  
Keyword(s):  
Fundic Gland ◽  
Advanced Carcinoma ◽  
Gland Area ◽  
Fundic Gland Area

Experimental gastric ulcers in the fundic gland area following transplantation of the gastric-wall pedicles

1976 ◽  
Vol 6 (3) ◽  
pp. 103-108
Author(s):  
Yuhiko Kurata ◽  
Motomu Kodama ◽  
Haruo Ezaki
Keyword(s):  
Gastric Wall ◽  
Gastric Ulcers ◽  
Fundic Gland ◽  
Gland Area ◽  
Fundic Gland Area

scholarly journals Gastric Ulcers in the Fundic Gland Area

1976 ◽  
Vol 71 (1) ◽  
pp. 16-18 ◽  
Author(s):  
Masaharu Tatsuta ◽  
Shigeru Okuda
Keyword(s):  
Gastric Ulcers ◽  
Fundic Gland ◽  
Gland Area ◽  
Fundic Gland Area

Neural isolation of the entire canine stomach in vivo: effects on motility

1989 ◽  
Vol 257 (1) ◽  
pp. G30-G40 ◽  
Author(s):  
J. A. Van Lier Ribbink ◽  
M. G. Sarr ◽  
M. Tanaka
Keyword(s):  
Small Intestine ◽  
Motor Activity ◽  
Motor Pattern ◽  
Phase Iii ◽  
Myoelectric Activity ◽  
Motor Patterns ◽  
Gastric Motor Activity ◽  
Plasma Motilin ◽  
In Vivo Effects

This study was designed to determine the effects of transection of all extrinsic and enteric neural continuity to the entire stomach on motility patterns of the stomach and small intestine. Five dogs were subjected to a model of orthotopic autotransplantation of the stomach to achieve an in vivo, "neurally isolated" stomach. Manometric catheters and serosal electrodes were implanted. A cyclic motor pattern occurred during fasting and was closely coordinated temporally with the migrating motor complex (MMC) in the small bowel. The period of the cyclic gastric motor activity did not differ from the period of the MMC in the small intestine [121 +/- 8 vs 124 +/- 10 (means +/- SE) min, P = 0.4], but the periods of both were greater than in control dogs (93 +/- 5 min, P less than 0.05). Tachygastria accounted for 36 +/- 13% of fasting myoelectric activity in the neurally isolated dogs and for less than 1% in control dogs. Plasma concentration of motilin was greatest during the phase III-like gastric motor activity; exogenous motilin induced premature phase III-like activity in the stomach and small intestine. Feeding abolished the cyclic motor activity in the stomach and decreased plasma motilin concentration. These data suggest that hormonal factors, and not extrinsic or intrinsic neural continuity to the stomach, may control both the initiation of a cyclic interdigestive gastric motor pattern and its temporal coordination with motor patterns in the small intestine.

Secretion of Acid and Pepsin in Response to Distention of Vagally Innervated Fundic Gland Area in Dogs

1962 ◽  
Vol 42 (6) ◽  
pp. 718-721 ◽  
Author(s):  
Morton I. Grossman
Keyword(s):  
Fundic Gland ◽  
Gland Area ◽  
Fundic Gland Area

scholarly journals Type of IIc Early Gastric Differentiated Carcinoma in Fundic Gland Area, Report of a Case

1995 ◽  
Vol 47 (0) ◽  
pp. 174-175
Author(s):  
Masayuki Inada ◽  
Kouichi Tokushige ◽  
Yasuo Okura ◽  
Gorou Yamaki ◽  
Kazuo Nomoto ◽  
...  
Keyword(s):  
Fundic Gland ◽  
Gland Area ◽  
Fundic Gland Area

scholarly journals A Novel Methodology For Isolation and Primary Culture of Swine Gastric Epithelial Cells.

2020 ◽  
Author(s):  
Henry Bautista-Amorocho ◽  
Jorge Alexander Silva-Sayago ◽  
Diego A. Goyeneche-Patino ◽  
Tania Liseth Pérez-Cala ◽  
Fabio Macías-Gómez ◽  
...  
Keyword(s):  
Growth Factors ◽  
Epithelial Cells ◽  
Protease Inhibitors ◽  
Swine Model ◽  
Type I ◽  
Fundic Gland ◽  
Gastric Epithelial Cells ◽  
Gland Area ◽  
Fundic Gland Area ◽  
Functional Phenotype

Abstract Background: Culture of primary epithelial cells has a great advantage over tumor-derived or immortal cells lines since functional phenotype and genetic makeup are preserved. Swine model has proved to be helpful and reliable as a surrogate model in human diseases. Several porcine cell lines have been established from a variety of tissues and shown to extensively contribute to the current understanding of several pathologies, including cancer. However, few protocols for the isolation and culture swine gastric epithelial cells with phenotype preservation have been described. Therefore, the objective of this research was to develop a new methodology for establishing a primary cell culture from the fundic gland area of the porcine stomach.Results: Enzymatic disaggregation of gastric tissue by using a combination of collagenase type I and dispase II, protease inhibitors (soybean trypsin inhibitor and bovine serum albumin), and antioxidants (Dithiothreitol) allowed the isolation of gastric epithelial cells from the fundic gland area with viability > 90% during the incubation period. Gastric epithelial cells cultured in RPMI 1640, DMEM HG, and DMEM/F12 media did not lead to cell adhesion, cluster formation and cell proliferation. By contrast, Williams’ medium supplemented with growth factors supports the confluence and proliferation of a pure epithelial cell monolayer after 10 days of incubation at 37oC in a 5% CO2 incubator. Mucin-producing cell phenotype of primary isolates was confirmed by PAS staining as well as the expression of MUC1 and MUC20 genes by RT-PCR and DNAc sequencing. Swine Gastric epithelial cells also showed origin-specific markers such as epithelial membrane antigen (EMA), cytokeratin cocktail (AE1/AE3) and cytokeratin 18 (CK-18) detected by immunohistochemistry and immunofluorescence, respectively. Conclusions: A new methodology was successfully established for the isolation of primary gastric epithelial cells from the fundic gland area in a swine model, based on a combination of tissue specific proteases, protease inhibitors, and antioxidants. The formulation of Williams’ medium with growth factors for epithelial cells supports the adherence and maintains the functional phenotype of primary cells, which was confirmed by mucin production and expression of typical epithelial markers in the long term.

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