ENHANCED RESOLUTION IN CONFOCAL MICROSCOPY BY USING CALCULATED THREE DIMENSIONAL POINT SPREAD FUNCTIONS

2000 ◽  
Author(s):  
MARTIN T. A. BOS ◽  
JOOST H. J. VAN OPHEUSDEN
Keyword(s):  
Confocal Microscopy ◽  
Three Dimensional ◽  
Point Spread Functions ◽  
Point Spread ◽  
Enhanced Resolution

scholarly journals Analyzing engineered point spread functions using phasor-based single-molecule localization microscopy

2020 ◽  
Author(s):  
Koen J.A. Martens ◽  
Abbas Jabermoradi ◽  
Suyeon Yang ◽  
Johannes Hohlbein
Keyword(s):  
Saddle Point ◽  
Single Molecule ◽  
Software Package ◽  
Double Helix ◽  
Three Dimensional ◽  
Fourier Plane ◽  
Point Spread Functions ◽  
Localization Microscopy ◽  
Point Spread ◽  
Single Molecule Localization Microscopy

The point spread function (PSF) of single molecule emitters can be engineered in the Fourier plane to encode three-dimensional localization information, creating double-helix, saddle-point or tetra-pod PSFs. Here, we describe and assess adaptations of the phasor-based single-molecule localization microscopy (pSMLM) algorithm to localize single molecules using these PSFs with sub-pixel accuracy. For double-helix, pSMLM identifies the two individual lobes and uses their relative rotation for obtaining z-resolved localizations, while for saddle-point or tetra-pod, a novel phasor-based deconvolution approach is used. The pSMLM software package delivers similar precision and recall rates to the best-in-class software package (SMAP) at signal-to-noise ratios typical for organic fluorophores. pSMLM substantially improves the localization rate by a factor of 2 - 4x on a standard CPU, with 1-1.5·104 (double-helix) or 2.5·105 (saddle-point/tetra-pod) localizations/second.

scholarly journals Three-dimensional hologram calculations using blocked radial and windmill point spread functions

2021 ◽  
Author(s):  
Daiki Yasuki ◽  
Tomoyoshi Shimobaba ◽  
Michal Makowski ◽  
David Blinder ◽  
Jaroslaw Suszek ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Point Spread Functions ◽  
Point Spread

scholarly journals Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions

Nano Letters ◽  
2015 ◽  
Vol 15 (6) ◽  
pp. 4194-4199 ◽  
Author(s):  
Yoav Shechtman ◽  
Lucien E. Weiss ◽  
Adam S. Backer ◽  
Steffen J. Sahl ◽  
W. E. Moerner
Keyword(s):  
Particle Tracking ◽  
Three Dimensional ◽  
Point Spread Functions ◽  
Point Spread ◽  
Multiple Particle Tracking ◽  
Multiple Particle

Three-Dimensional Digital Confocal Raman Microscopy

1993 ◽  
Vol 47 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Anurag Govil ◽  
David M. Pallister ◽  
Michael D. Morris
Keyword(s):  
Confocal Microscopy ◽  
Image Restoration ◽  
Point Spread Function ◽  
Three Dimensional ◽  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
Point Spread ◽  
Restoration Technique ◽  
Spread Function ◽  
Confocal Raman

We describe an iterative image restoration technique which functions as digital confocal microscopy for Raman images. We deconvolute the lateral and axial components of the microscope point spread function from a series of optical sections, to generate a stack of well-resolved Raman images which describe the three-dimensional topology of a sample. The technique provides an alternative to confocal microscopy for three-dimensional microscopic Raman imaging.

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